Gene description for Rps8
Gene name ribosomal protein S8
Gene symbol Rps8
Other names/aliases -
Species Mus musculus
 Database cross references - Rps8
ExoCarta ExoCarta_20116
Vesiclepedia VP_20116
Entrez Gene 20116
UniProt P62242  
 Rps8 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rps8
Molecular Function
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
Biological Process
    maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000462 IBA
    cytoplasmic translation GO:0002181 NAS
    ribosomal small subunit biogenesis GO:0042274 ISO
    ribosomal small subunit biogenesis GO:0042274 ISS
Subcellular Localization
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 NAS
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    membrane GO:0016020 IEA
    cytosolic ribosome GO:0022626 ISO
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 ISO
    cytosolic small ribosomal subunit GO:0022627 NAS
    small-subunit processome GO:0032040 ISO
    small-subunit processome GO:0032040 ISS
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Rps8 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rps8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rps7 20115
Co-fractionation Mus musculus
3 Rpl11 67025
Co-fractionation Mus musculus
4 Psmd8 57296
Co-fractionation Mus musculus
5 Rps18 20084
Co-fractionation Mus musculus
6 Rps3 27050
Co-fractionation Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
8 Rps10 67097
Co-fractionation Mus musculus
9 Rpl13a 22121
Co-fractionation Mus musculus
10 Atxn2 20239
Co-fractionation Mus musculus
11 Rps15 20054
Co-fractionation Mus musculus
12 Stau1  
Co-fractionation Mus musculus
13 Rpl15 66480
Co-fractionation Mus musculus
14 Rplp2 67186
Co-fractionation Mus musculus
15 Actg1 11465
Co-fractionation Mus musculus
16 Rps4x 20102
Co-fractionation Mus musculus
17 Rpl14 67115
Co-fractionation Mus musculus
18 Rps2 16898
Co-fractionation Mus musculus
19 Trove2 20822
Co-fractionation Mus musculus
20 Gapdh 14433
Co-fractionation Mus musculus
21 Psmd13 23997
Co-fractionation Mus musculus
22 Rps23 66475
Co-fractionation Mus musculus
23 Rpl7a 27176
Co-fractionation Mus musculus
24 Ybx1 22608
Co-fractionation Mus musculus
25 Rpl32 19951
Co-fractionation Mus musculus
26 Wibg  
Co-fractionation Mus musculus
27 Rpl6 19988
Co-fractionation Mus musculus
28 Kctd13  
Affinity Capture-MS Mus musculus
29 Rps19 20085
Co-fractionation Mus musculus
30 Rps3a1 20091
Co-fractionation Mus musculus
31 Tfe3  
Affinity Capture-MS Mus musculus
32 Mrpl41  
Two-hybrid Mus musculus
33 Tpt1 22070
Co-fractionation Mus musculus
34 Rps11 27207
Co-fractionation Mus musculus
35 Ncl 17975
Co-fractionation Mus musculus
36 Rps24 20088
Co-fractionation Mus musculus
37 Actb 11461
Co-fractionation Mus musculus
38 Rpl19 19921
Co-fractionation Mus musculus
39 Gnb2l1 14694
Co-fractionation Mus musculus
40 Rpl13 270106
Co-fractionation Mus musculus
41 Rpl35a 57808
Co-fractionation Mus musculus
42 Rpl22 19934
Co-fractionation Mus musculus
43 Mettl21e  
Affinity Capture-MS Mus musculus
44 Rpl4 67891
Co-fractionation Mus musculus
45 Tmem173  
Proximity Label-MS Mus musculus
46 Fzr1  
Affinity Capture-MS Mus musculus
47 Pabpc1 18458
Co-fractionation Mus musculus
48 Rps6 20104
Co-fractionation Mus musculus
49 Rps13 68052
Co-fractionation Mus musculus
50 Rps5 20103
Co-fractionation Mus musculus
51 Rps9 76846
Co-fractionation Mus musculus
52 Hnrnpab 15384
Co-fractionation Mus musculus
53 Rps21 66481
Co-fractionation Mus musculus
54 Rpl23 65019
Co-fractionation Mus musculus
55 Rps15a 267019
Co-fractionation Mus musculus
56 Rps28 54127
Co-fractionation Mus musculus
57 Rplp0 11837
Co-fractionation Mus musculus
58 Syncrip 56403
Co-fractionation Mus musculus
59 Rpl35 66489
Co-fractionation Mus musculus
60 Psmd3 22123
Co-fractionation Mus musculus
61 Rpl18a 76808
Co-fractionation Mus musculus
62 Rpl26 19941
Co-fractionation Mus musculus
63 Rps20 67427
Co-fractionation Mus musculus
64 Dhx9 13211
Co-fractionation Mus musculus
65 Rpl23a 268449
Co-fractionation Mus musculus
66 Rps14 20044
Co-fractionation Mus musculus
67 Eed  
Affinity Capture-MS Mus musculus
68 Rpsa 16785
Co-fractionation Mus musculus
69 Eef1b2 55949
Co-fractionation Mus musculus
70 Larp1  
Co-fractionation Mus musculus
71 Rps16 20055
Co-fractionation Mus musculus
72 Rpl39 67248
Co-fractionation Mus musculus
73 Hba 15121
Co-fractionation Mus musculus
74 Rpl8 26961
Co-fractionation Mus musculus
75 Rps12 20042
Co-fractionation Mus musculus
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