Gene description for Rpl35a
Gene name ribosomal protein L35A
Gene symbol Rpl35a
Other names/aliases 2810431L15Rik
Rpl35
Species Mus musculus
 Database cross references - Rpl35a
ExoCarta ExoCarta_57808
Vesiclepedia VP_57808
Entrez Gene 57808
UniProt O55142  
 Rpl35a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl35a
Molecular Function
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
Biological Process
    cytoplasmic translation GO:0002181 IBA
    cytoplasmic translation GO:0002181 NAS
    rRNA processing GO:0006364 IEA
    rRNA processing GO:0006364 ISO
    ribosomal large subunit biogenesis GO:0042273 IBA
    ribosomal large subunit biogenesis GO:0042273 IEA
    ribosomal large subunit biogenesis GO:0042273 ISO
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rpl35a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl35a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Sf3a3 75062
Co-fractionation Mus musculus
3 Dhx29  
Co-fractionation Mus musculus
4 Rpl13a 22121
Co-fractionation Mus musculus
5 Rps4x 20102
Co-fractionation Mus musculus
6 Rps8 20116
Co-fractionation Mus musculus
7 Scyl2  
Co-fractionation Mus musculus
8 Rpl6 19988
Co-fractionation Mus musculus
9 Kctd13  
Affinity Capture-MS Mus musculus
10 Tmem165  
Co-fractionation Mus musculus
11 Tfe3  
Affinity Capture-MS Mus musculus
12 Rps24 20088
Co-fractionation Mus musculus
13 Lrrc59  
Co-fractionation Mus musculus
14 Rpl22 19934
Co-fractionation Mus musculus
15 Tmem173  
Proximity Label-MS Mus musculus
16 Fzr1  
Affinity Capture-MS Mus musculus
17 Rps6 20104
Co-fractionation Mus musculus
18 Gtf2f1  
Co-fractionation Mus musculus
19 Rpl7 19989
Co-fractionation Mus musculus
20 Plod3 26433
Co-fractionation Mus musculus
21 Dicer1  
Co-fractionation Mus musculus
22 AI314180 230249
Co-fractionation Mus musculus
23 Rps3a1 20091
Co-fractionation Mus musculus
24 Ppwd1  
Co-fractionation Mus musculus
25 Rpl13 270106
Co-fractionation Mus musculus
26 Eed  
Affinity Capture-MS Mus musculus
27 Rpl15 66480
Co-fractionation Mus musculus
28 Top1 21969
Co-fractionation Mus musculus
29 Abce1 24015
Co-fractionation Mus musculus
30 Fancd2  
Affinity Capture-MS Mus musculus
31 Usp46  
Co-fractionation Mus musculus
32 Tcf25  
Co-fractionation Mus musculus
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