Gene description for Rpl7
Gene name ribosomal protein L7
Gene symbol Rpl7
Other names/aliases Rpl7a
Surf-3
Species Mus musculus
 Database cross references - Rpl7
ExoCarta ExoCarta_19989
Entrez Gene 19989
UniProt P14148  
 Rpl7 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for Rpl7
Molecular Function
    protein homodimerization activity GO:0042803 ISO
    poly(A) RNA binding GO:0044822 ISO
    DNA binding GO:0003677 ISO
    RNA binding GO:0003723 IEA
    structural constituent of ribosome GO:0003735 IC
    mRNA binding GO:0003729 ISO
Biological Process
    translation GO:0006412 IC
    ribosomal large subunit biogenesis GO:0042273 ISO
    rRNA processing GO:0006364 ISO
Subcellular Localization
    polysome GO:0005844 IDA
    cytoplasm GO:0005737 ISO
    nucleolus GO:0005730 ISO
    nucleus GO:0005634 ISO
    extracellular exosome GO:0070062 ISO
    ribosome GO:0005840 IEA
    membrane GO:0016020 ISO
    cytosolic large ribosomal subunit GO:0022625 ISO
    focal adhesion GO:0005925 ISO
    ribonucleoprotein complex GO:0030529 ISO
 Experiment description of studies that identified Rpl7 in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rpl7
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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