Gene description for Npm1
Gene name nucleophosmin 1
Gene symbol Npm1
Other names/aliases B23
NO38
Npm
Species Mus musculus
 Database cross references - Npm1
ExoCarta ExoCarta_18148
Vesiclepedia VP_18148
Entrez Gene 18148
UniProt Q61937  
 Npm1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Npm1
Molecular Function
    core promoter sequence-specific DNA binding GO:0001046 ISO
    p53 binding GO:0002039 ISO
    DNA binding GO:0003677 IEA
    DNA binding GO:0003677 ISO
    chromatin binding GO:0003682 IBA
    transcription coactivator activity GO:0003713 ISO
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IDA
    RNA binding GO:0003723 ISO
    protein kinase inhibitor activity GO:0004860 ISO
    protein kinase inhibitor activity GO:0004860 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 ISO
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISO
    rRNA binding GO:0019843 IDA
    rRNA binding GO:0019843 ISO
    enzyme binding GO:0019899 ISO
    protein kinase binding GO:0019901 ISO
    Tat protein binding GO:0030957 ISO
    histone binding GO:0042393 IBA
    histone binding GO:0042393 ISO
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    ribosomal large subunit binding GO:0043023 ISO
    ribosomal small subunit binding GO:0043024 ISO
    protein kinase B binding GO:0043422 ISO
    NF-kappaB binding GO:0051059 ISO
    NF-kappaB binding GO:0051059 ISS
    unfolded protein binding GO:0051082 ISO
    unfolded protein binding GO:0051082 ISS
    DNA-binding transcription factor binding GO:0140297 ISO
Biological Process
    ribosomal large subunit export from nucleus GO:0000055 IBA
    ribosomal large subunit export from nucleus GO:0000055 IMP
    ribosomal small subunit export from nucleus GO:0000056 IBA
    ribosomal small subunit export from nucleus GO:0000056 IDA
    cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000448 ISO
    regulation of cell growth GO:0001558 IDA
    DNA repair GO:0006281 ISO
    DNA repair GO:0006281 ISS
    nucleosome assembly GO:0006334 ISO
    chromatin remodeling GO:0006338 IBA
    cell volume homeostasis GO:0006884 IDA
    cell volume homeostasis GO:0006884 IMP
    nucleocytoplasmic transport GO:0006913 IDA
    nucleocytoplasmic transport GO:0006913 ISO
    centrosome cycle GO:0007098 ISO
    centrosome cycle GO:0007098 ISS
    protein localization GO:0008104 IMP
    protein localization GO:0008104 ISO
    positive regulation of cell population proliferation GO:0008284 IDA
    positive regulation of cell population proliferation GO:0008284 IMP
    positive regulation of cell population proliferation GO:0008284 ISO
    negative regulation of cell population proliferation GO:0008285 ISO
    negative regulation of cell population proliferation GO:0008285 ISS
    rRNA transcription GO:0009303 ISO
    positive regulation of biosynthetic process GO:0009891 IDA
    positive regulation of biosynthetic process GO:0009891 IMP
    post-transcriptional regulation of gene expression GO:0010608 IMP
    negative regulation of gene expression GO:0010629 IGI
    negative regulation of cardiac muscle cell apoptotic process GO:0010667 ISO
    regulation of centrosome duplication GO:0010824 IBA
    regulation of centrosome duplication GO:0010824 IMP
    positive regulation of centrosome duplication GO:0010825 IGI
    negative regulation of centrosome duplication GO:0010826 ISO
    positive regulation of protein ubiquitination GO:0031398 IMP
    regulation of protein stability GO:0031647 IMP
    protein destabilization GO:0031648 IMP
    regulation of endodeoxyribonuclease activity GO:0032071 ISO
    regulation of endodeoxyribonuclease activity GO:0032071 ISS
    cellular response to UV GO:0034644 ISO
    ribosomal large subunit biogenesis GO:0042273 IBA
    ribosomal large subunit biogenesis GO:0042273 IDA
    ribosomal large subunit biogenesis GO:0042273 IMP
    ribosomal small subunit biogenesis GO:0042274 IBA
    ribosomal small subunit biogenesis GO:0042274 IDA
    ribosomal small subunit biogenesis GO:0042274 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of apoptotic process GO:0043066 ISS
    regulation of DNA damage response, signal transduction by p53 class mediator GO:0043516 IGI
    regulation of neuron apoptotic process GO:0043523 ISO
    negative regulation of neuron apoptotic process GO:0043524 ISO
    negative regulation of protein kinase activity by regulation of protein phosphorylation GO:0044387 ISO
    negative regulation of protein kinase activity by regulation of protein phosphorylation GO:0044387 ISS
    positive regulation of translation GO:0045727 ISO
    positive regulation of translation GO:0045727 ISS
    positive regulation of DNA replication GO:0045740 ISO
    positive regulation of protein kinase activity GO:0045860 IDA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of DNA-templated transcription GO:0045893 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    regulation of centriole replication GO:0046599 ISO
    regulation of centriole replication GO:0046599 ISS
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IDA
    negative regulation of epithelial cell proliferation GO:0050680 ISO
    protein stabilization GO:0050821 IGI
    protein stabilization GO:0050821 IMP
    protein stabilization GO:0050821 IMP
    positive regulation of DNA metabolic process GO:0051054 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    regulation of cell cycle GO:0051726 IMP
    regulation of endoribonuclease activity GO:0060699 ISO
    regulation of endoribonuclease activity GO:0060699 ISS
    regulation of eIF2 alpha phosphorylation by dsRNA GO:0060735 ISO
    regulation of eIF2 alpha phosphorylation by dsRNA GO:0060735 ISS
    cellular senescence GO:0090398 ISS
    regulation of mRNA stability involved in cellular response to UV GO:1902629 ISO
    positive regulation of cell cycle G2/M phase transition GO:1902751 ISO
    positive regulation of cell cycle G2/M phase transition GO:1902751 ISS
    positive regulation of protein localization to nucleolus GO:1904751 IDA
    positive regulation of protein localization to nucleolus GO:1904751 IMP
Subcellular Localization
    granular component GO:0001652 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    cytosol GO:0005829 IDA
    large ribosomal subunit GO:0015934 IDA
    small ribosomal subunit GO:0015935 IDA
    nuclear matrix GO:0016363 ISO
    nuclear speck GO:0016607 IDA
    spindle pole centrosome GO:0031616 ISO
    protein-containing complex GO:0032991 ISO
    protein-DNA complex GO:0032993 ISO
    ribonucleoprotein complex GO:1990904 IBA
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Npm1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Npm1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
2 Hnrnph2 56258
Affinity Capture-MS Mus musculus
3 Fxr1 14359
Affinity Capture-MS Mus musculus
4 Cpsf6  
Affinity Capture-MS Mus musculus
5 Rpl22 19934
Affinity Capture-MS Mus musculus
6 Rpl13a 22121
Affinity Capture-MS Mus musculus
7 PARP1 142
Affinity Capture-Western Homo sapiens
8 Vim 22352
Affinity Capture-MS Mus musculus
9 Ebpl  
Affinity Capture-MS Mus musculus
10 Cdk2  
Biochemical Activity Mus musculus
11 Trp53  
Affinity Capture-Western Mus musculus
12 Rpl27 19942
Affinity Capture-MS Mus musculus
13 Atxn1  
Proximity Label-MS Mus musculus
14 Kcna3  
Affinity Capture-MS Mus musculus
15 Kctd13  
Affinity Capture-MS Mus musculus
16 Ddx5 13207
Affinity Capture-MS Mus musculus
17 Tfe3  
Affinity Capture-MS Mus musculus
18 Chaf1a  
Affinity Capture-MS Mus musculus
19 Rps6 20104
Affinity Capture-MS Mus musculus
20 Ncl 17975
Affinity Capture-MS Mus musculus
21 Mecp2  
Affinity Capture-MS Mus musculus
22 Rps7 20115
Affinity Capture-MS Mus musculus
23 PARP2  
Affinity Capture-Western Homo sapiens
24 Mettl21e  
Affinity Capture-MS Mus musculus
25 Rpl4 67891
Affinity Capture-MS Mus musculus
26 Tmem173  
Proximity Label-MS Mus musculus
27 Fzr1  
Affinity Capture-MS Mus musculus
28 Hnrnpa1 15382
Affinity Capture-MS Mus musculus
29 Eef2 13629
Affinity Capture-MS Mus musculus
30 Rpl7 19989
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
31 Rock2  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
32 Nup62  
Affinity Capture-MS Mus musculus
33 Eif2ak2 19106
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
34 Nup50  
Affinity Capture-MS Mus musculus
35 MAPT  
Reconstituted Complex Homo sapiens
36 Rps5 20103
Affinity Capture-MS Mus musculus
37 Rps3a1 20091
Affinity Capture-MS Mus musculus
38 HACE1 57531
Affinity Capture-MS Homo sapiens
39 Ddx1 104721
Affinity Capture-MS Mus musculus
40 Dhx9 13211
Affinity Capture-MS Mus musculus
41 Brca1  
Affinity Capture-MS Mus musculus
42 Eed  
Affinity Capture-MS Mus musculus
43 Parp2  
Affinity Capture-Western Mus musculus
44 Fbxw7  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
45 Rpl5 100503670
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
46 Fancd2  
Affinity Capture-MS Mus musculus
47 Tcf3  
Affinity Capture-MS Mus musculus
48 Srsf2 20382
Co-localization Mus musculus
View the network image/svg+xml



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