Gene description for Npm1
Gene name nucleophosmin 1
Gene symbol Npm1
Other names/aliases B23
NO38
Npm
Species Mus musculus
 Database cross references - Npm1
ExoCarta ExoCarta_18148
Vesiclepedia VP_18148
Entrez Gene 18148
UniProt Q61937  
 Npm1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Npm1
Molecular Function
    protein N-terminus binding GO:0047485 IPI
    ribosomal small subunit binding GO:0043024 ISO
    protein kinase binding GO:0019901 ISO
    protein homodimerization activity GO:0042803 ISO
    RNA binding GO:0003723 ISO
    protein heterodimerization activity GO:0046982 ISO
    protein binding GO:0005515 IPI
    NF-kappaB binding GO:0051059 ISO
    histone binding GO:0042393 ISO
    Tat protein binding GO:0030957 ISO
    enzyme binding GO:0019899 ISO
    nucleic acid binding GO:0003676 IEA
    poly(A) RNA binding GO:0044822 ISO
    unfolded protein binding GO:0051082 ISO
    ribosomal large subunit binding GO:0043023 ISO
    transcription coactivator activity GO:0003713 ISO
    rRNA binding GO:0019843 IDA
    DNA binding GO:0003677 ISO
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISO
    protein kinase inhibitor activity GO:0004860 ISO
Biological Process
    negative regulation of cell proliferation GO:0008285 ISO
    regulation of endoribonuclease activity GO:0060699 ISO
    positive regulation of DNA metabolic process GO:0051054 ISO
    regulation of DNA damage response, signal transduction by p53 class mediator GO:0043516 IGI
    response to stress GO:0006950 ISO
    cell growth GO:0016049 IDA
    DNA repair GO:0006281 ISO
    nucleocytoplasmic transport GO:0006913 ISO
    negative regulation of protein kinase activity by regulation of protein phosphorylation GO:0044387 ISO
    positive regulation of cellular biosynthetic process GO:0031328 IMP
    positive regulation of DNA replication GO:0045740 ISO
    ribosomal large subunit export from nucleus GO:0000055 IMP
    regulation of endodeoxyribonuclease activity GO:0032071 ISO
    cell volume homeostasis GO:0006884 IMP
    regulation of centriole replication GO:0046599 ISO
    ribosomal small subunit biogenesis GO:0042274 IMP
    negative regulation of centrosome duplication GO:0010826 ISO
    positive regulation of catalytic activity GO:0043085 ISO
    cell aging GO:0007569 ISO
    protein oligomerization GO:0051259 ISO
    centrosome cycle GO:0007098 ISO
    rRNA export from nucleus GO:0006407 IMP
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IDA
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of protein kinase activity GO:0045860 IDA
    protein localization GO:0008104 ISO
    ribosomal large subunit biogenesis GO:0042273 IMP
    regulation of centrosome duplication GO:0010824 IMP
    positive regulation of centrosome duplication GO:0010825 IGI
    protein destabilization GO:0031648 IMP
    ribosomal small subunit export from nucleus GO:0000056 IDA
    positive regulation of cell proliferation GO:0008284 IMP
    protein homooligomerization GO:0051260 ISO
    regulation of eIF2 alpha phosphorylation by dsRNA GO:0060735 ISO
    nucleosome assembly GO:0006334 ISO
    positive regulation of translation GO:0045727 ISO
    regulation of cell cycle GO:0051726 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    regulation of neuron apoptotic process GO:0043523 ISO
Subcellular Localization
    centrosome GO:0005813 ISO
    nucleoplasm GO:0005654 ISO
    granular component GO:0001652 IDA
    nucleus GO:0005634 ISO
    focal adhesion GO:0005925 ISO
    cell GO:0005623 IMP
    cytosol GO:0005829 IDA
    intracellular GO:0005622 IMP
    nucleolus GO:0005730 ISO
    large ribosomal subunit GO:0015934 IDA
    membrane GO:0016020 ISO
    cytoskeleton GO:0005856 IEA
    small ribosomal subunit GO:0015935 IDA
    ribonucleoprotein complex GO:0030529 ISO
    cytoplasm GO:0005737 ISO
    nuclear speck GO:0016607 IDA
    spindle pole centrosome GO:0031616 ISO
 Experiment description of studies that identified Npm1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Npm1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Npm1 is involved
PathwayEvidenceSource
Deposition of new CENPA-containing nucleosomes at the centromere IEA Reactome





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