Gene description for Srsf2
Gene name serine/arginine-rich splicing factor 2
Gene symbol Srsf2
Other names/aliases D11Wsu175e
MRF-1
Pr264
SC35
Sfrs10
Sfrs2
Species Mus musculus
 Database cross references - Srsf2
ExoCarta ExoCarta_20382
Vesiclepedia VP_20382
Entrez Gene 20382
UniProt Q62093  
 Srsf2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Srsf2
Molecular Function
    pre-mRNA binding GO:0036002 IDA
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 ISO
    nucleic acid binding GO:0003676 IEA
    RNA binding GO:0003723 IEA
Biological Process
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IDA
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
Subcellular Localization
    cytoplasm GO:0005737 ISO
    nucleoplasm GO:0005654 ISO
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 ISO
    nuclear speck GO:0016607 ISO
    spliceosomal complex GO:0005681 IDA
 Experiment description of studies that identified Srsf2 in exosomes
1
Experiment ID 907
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
EM
EV Biophysical techniques
CD63
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Srsf2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fus  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Srsf2 is involved
No pathways found





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