Gene description for Ncl
Gene name nucleolin
Gene symbol Ncl
Other names/aliases B530004O11Rik
C23
D0Nds28
D1Nds28
Nucl
Species Mus musculus
 Database cross references - Ncl
ExoCarta ExoCarta_17975
Vesiclepedia VP_17975
Entrez Gene 17975
UniProt P09405  
 Ncl identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ncl
Molecular Function
    single-stranded DNA binding GO:0003697 ISO
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IDA
    RNA binding GO:0003723 ISO
    RNA binding GO:0003723 ISS
    signaling receptor binding GO:0005102 ISO
    calcium ion binding GO:0005509 ISO
    protein binding GO:0005515 IPI
    selenocysteine insertion sequence binding GO:0035368 ISO
    rRNA primary transcript binding GO:0042134 ISO
    telomeric DNA binding GO:0042162 ISO
    telomeric DNA binding GO:0042162 ISS
    histone binding GO:0042393 ISO
    PH domain binding GO:0042731 IPI
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    laminin binding GO:0043236 ISO
    insulin receptor substrate binding GO:0043560 IPI
    sequence-specific DNA binding GO:0043565 ISO
    DNA topoisomerase binding GO:0044547 IEA
    DNA topoisomerase binding GO:0044547 ISO
    mRNA 5'-UTR binding GO:0048027 IEA
    mRNA 5'-UTR binding GO:0048027 ISO
    ErbB-4 class receptor binding GO:1990631 ISO
Biological Process
    angiogenesis GO:0001525 IEA
    angiogenesis GO:0001525 ISO
    endocytosis GO:0006897 ISO
    negative regulation of translation GO:0017148 IEA
    negative regulation of translation GO:0017148 ISO
    positive regulation of interleukin-6 production GO:0032755 ISO
    positive regulation of tumor necrosis factor production GO:0032760 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    negative regulation of insulin receptor signaling pathway GO:0046627 IDA
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IBA
    regulation of peptidyl-tyrosine phosphorylation GO:0050730 IDA
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 IDA
    positive regulation of transcription of nucleolar large rRNA by RNA polymerase I GO:1901838 IEA
    positive regulation of transcription of nucleolar large rRNA by RNA polymerase I GO:1901838 ISO
    cellular response to leukemia inhibitory factor GO:1990830 IEP
    regulation of rRNA processing GO:2000232 ISO
Subcellular Localization
    cornified envelope GO:0001533 IDA
    fibrillar center GO:0001650 ISO
    dense fibrillar component GO:0001651 ISO
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 IBA
    chromosome GO:0005694 IEA
    chromosome GO:0005694 ISO
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 ISO
    cell cortex GO:0005938 IEA
    cell cortex GO:0005938 ISO
    cell surface GO:0009986 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Ncl in exosomes
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
EM
Biophysical techniques
CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
7
Experiment ID 188
MISEV standards
EM
Biophysical techniques
GAPDH|UCHL1|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ncl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Rpl28 19943
Co-fractionation Mus musculus
3 Eprs 107508
Co-fractionation Mus musculus
4 Rplp0 11837
Co-fractionation Mus musculus
5 Ncbp1 433702
Co-fractionation Mus musculus
6 Eif3i 54709
Affinity Capture-Western Mus musculus
7 Ina  
Co-fractionation Mus musculus
8 Pidd1  
Two-hybrid Mus musculus
9 Rpl13a 22121
Co-fractionation Mus musculus
10 Sarnp 66118
Co-fractionation Mus musculus
11 Stau1  
Co-fractionation Mus musculus
12 Pou5f1  
Affinity Capture-Luminescence Mus musculus
13 Ilf2 67781
Co-fractionation Mus musculus
14 Rps4x 20102
Co-fractionation Mus musculus
15 Rps8 20116
Co-fractionation Mus musculus
16 Mapt  
Affinity Capture-MS Mus musculus
17 Atxn1  
Proximity Label-MS Mus musculus
18 Rpl6 19988
Co-fractionation Mus musculus
19 Kctd13  
Affinity Capture-MS Mus musculus
20 Ddx5 13207
Co-fractionation Mus musculus
21 Iqgap1 29875
Co-fractionation Mus musculus
22 Rps3a1 20091
Co-fractionation Mus musculus
23 Tfe3  
Affinity Capture-MS Mus musculus
24 Cul3  
Affinity Capture-MS Mus musculus
25 Tpt1 22070
Affinity Capture-Western Mus musculus
Affinity Capture-Luminescence Mus musculus
26 Rps6 20104
Co-fractionation Mus musculus
27 Ddx6 13209
Co-fractionation Mus musculus
28 Ddx17 67040
Co-fractionation Mus musculus
29 Ywhae 22627
Affinity Capture-MS Mus musculus
30 Serbp1 66870
Co-fractionation Mus musculus
31 Mettl21e  
Affinity Capture-MS Mus musculus
32 Tmem173  
Proximity Label-MS Mus musculus
33 Fzr1  
Affinity Capture-MS Mus musculus
34 Ybx1 22608
Co-fractionation Mus musculus
35 Poldip3  
Co-fractionation Mus musculus
36 Rock2  
Co-fractionation Mus musculus
37 Ago2 239528
Co-fractionation Mus musculus
38 Hnrnpab 15384
Co-fractionation Mus musculus
39 Actn4 60595
Co-fractionation Mus musculus
40 Srsf2 20382
Co-fractionation Mus musculus
41 Unk  
Affinity Capture-RNA Mus musculus
42 Rhoa 11848
Co-fractionation Mus musculus
43 Syncrip 56403
Co-fractionation Mus musculus
44 TCF3  
Reconstituted Complex Homo sapiens
45 Npm1 18148
Affinity Capture-MS Mus musculus
46 Rpl26 19941
Co-fractionation Mus musculus
47 Ctsh  
Co-fractionation Mus musculus
48 Dhx9 13211
Co-fractionation Mus musculus
49 Tubb5 22154
Co-fractionation Mus musculus
50 Ilf3 16201
Co-fractionation Mus musculus
51 Eed  
Affinity Capture-MS Mus musculus
52 Fmr1  
Affinity Capture-MS Mus musculus
53 Atg16l1  
Affinity Capture-MS Mus musculus
54 Pcbp1 23983
Co-fractionation Mus musculus
55 Tcf3  
Affinity Capture-MS Mus musculus
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