Gene description for Ncl
Gene name nucleolin
Gene symbol Ncl
Other names/aliases B530004O11Rik
C23
D0Nds28
D1Nds28
Nucl
Species Mus musculus
 Database cross references - Ncl
ExoCarta ExoCarta_17975
Entrez Gene 17975
UniProt P09405  
 Ncl identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for Ncl
Molecular Function
    nucleic acid binding GO:0003676 IEA
    protein binding GO:0005515 IPI
    sequence-specific DNA binding GO:0043565 ISO
    selenocysteine insertion sequence binding GO:0035368 ISO
    identical protein binding GO:0042802 ISO
    ErbB-4 class receptor binding GO:1990631 ISO
    DNA binding GO:0003677 IEA
    calcium ion binding GO:0005509 ISO
    poly(A) RNA binding GO:0044822 ISO
    telomeric DNA binding GO:0042162 ISO
    nucleotide binding GO:0000166 IEA
    laminin binding GO:0043236 ISO
    histone binding GO:0042393 ISO
    receptor binding GO:0005102 ISO
    single-stranded DNA binding GO:0003697 ISO
    protein C-terminus binding GO:0008022 ISO
    RNA binding GO:0003723 ISO
    rRNA primary transcript binding GO:0042134 ISO
Biological Process
    positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter GO:1901838 ISO
    cellular response to lipopolysaccharide GO:0071222 ISO
    positive regulation of tumor necrosis factor production GO:0032760 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IGI
    regulation of rRNA processing GO:2000232 ISO
    endocytosis GO:0006897 ISO
    positive regulation of interleukin-6 secretion GO:2000778 ISO
    angiogenesis GO:0001525 ISO
    negative regulation of apoptotic process GO:0043066 ISO
Subcellular Localization
    membrane GO:0016020 ISO
    nucleolus GO:0005730 ISO
    nucleoplasm GO:0005654 ISO
    dense fibrillar component GO:0001651 ISO
    cell surface GO:0009986 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    nucleus GO:0005634 ISO
    fibrillar center GO:0001650 ISO
    cell cortex GO:0005938 ISO
    cytoplasm GO:0005737 ISO
    extracellular exosome GO:0070062 ISO
    ribonucleoprotein complex GO:0030529 ISO
 Experiment description of studies that identified Ncl in exosomes
1
Experiment ID 210
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Ncl
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ncl is involved
No pathways found





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