Gene description for Iqgap1
Gene name IQ motif containing GTPase activating protein 1
Gene symbol Iqgap1
Other names/aliases AA682088
D7Ertd237e
D7Ertd257e
mKIAA0051
Species Mus musculus
 Database cross references - Iqgap1
ExoCarta ExoCarta_29875
Vesiclepedia VP_29875
Entrez Gene 29875
UniProt Q9JKF1  
 Iqgap1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Microglia 16081791    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Iqgap1
Molecular Function
    protein serine/threonine kinase activator activity GO:0043539 ISO
    protein binding GO:0005515 IPI
    Rac GTPase binding GO:0048365 IPI
    protein complex binding GO:0032403 ISO
    Rho GTPase binding GO:0017048 IPI
    calmodulin binding GO:0005516 ISO
    GTPase activator activity GO:0005096 IEA
    protein kinase binding GO:0019901 ISO
    protein phosphatase binding GO:0019903 ISO
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISO
Biological Process
    cellular response to epidermal growth factor stimulus GO:0071364 ISO
    cellular response to fibroblast growth factor stimulus GO:0044344 IMP
    fibroblast growth factor receptor signaling pathway GO:0008543 IMP
    platelet-derived growth factor receptor signaling pathway GO:0048008 IMP
    cellular response to calcium ion GO:0071277 ISO
    negative regulation of dephosphorylation GO:0035305 IGI
    small GTPase mediated signal transduction GO:0007264 IEA
    regulation of GTPase activity GO:0043087 IEA
    cellular response to platelet-derived growth factor stimulus GO:0036120 IMP
    positive regulation of protein kinase activity GO:0045860 ISO
    signal transduction GO:0007165 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 ISO
    neuron projection extension GO:1990138 ISO
    regulation of cytokine production GO:0001817 IMP
    positive regulation of protein serine/threonine kinase activity GO:0071902 ISO
Subcellular Localization
    axon GO:0030424 ISO
    cytoplasm GO:0005737 ISO
    growth cone GO:0030426 ISO
    neuron projection GO:0043005 ISO
    cell leading edge GO:0031252 IGI
    extracellular exosome GO:0070062 ISO
    microtubule cytoskeleton GO:0015630 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    cell junction GO:0030054 ISO
    membrane GO:0016020 IDA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 ISO
    lateral plasma membrane GO:0016328 IDA
    ribonucleoprotein complex GO:0030529 IGI
    nucleoplasm GO:0005654 ISO
    midbody GO:0030496 ISO
    protein complex GO:0043234 ISO
    actin cytoskeleton GO:0015629 ISO
    plasma membrane GO:0005886 ISO
    focal adhesion GO:0005925 ISO
    cell-cell junction GO:0005911 IDA
    microtubule GO:0005874 ISO
 Experiment description of studies that identified Iqgap1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 14
MISEV standards
✔
EM
EV Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
EV Enriched markers
✔
DNM
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
7
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Iqgap1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Iqgap1 is involved
PathwayEvidenceSource
Nephrin interactions IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome





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