Gene description for Rhoa
Gene name ras homolog gene family, member A
Gene symbol Rhoa
Other names/aliases Arha
Arha1
Arha2
Species Mus musculus
 Database cross references - Rhoa
ExoCarta ExoCarta_11848
Vesiclepedia VP_11848
Entrez Gene 11848
UniProt Q9QUI0  
 Rhoa identified in exosomes derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rhoa
Molecular Function
    protein domain specific binding GO:0019904 ISO
    GTP binding GO:0005525 ISO
    Rho GDP-dissociation inhibitor binding GO:0051022 ISO
    nucleotide binding GO:0000166 IEA
    GTPase activity GO:0003924 TAS
    protein binding GO:0005515 IPI
    GDP binding GO:0019003 ISO
    myosin binding GO:0017022 ISO
Biological Process
    apical junction assembly GO:0043297 ISO
    actin cytoskeleton organization GO:0030036 IDA
    negative regulation of neuron differentiation GO:0045665 ISO
    stress fiber assembly GO:0043149 IDA
    positive regulation of neuron differentiation GO:0045666 ISO
    regulation of neuron projection development GO:0010975 IDA
    forebrain radial glial cell differentiation GO:0021861 IMP
    regulation of cell migration GO:0030334 ISO
    positive regulation of neuron apoptotic process GO:0043525 ISO
    cell adhesion GO:0007155 IMP
    cell cycle GO:0007049 IEA
    trabecula morphogenesis GO:0061383 IMP
    regulation of osteoblast proliferation GO:0033688 IMP
    androgen receptor signaling pathway GO:0030521 IDA
    positive regulation of stress fiber assembly GO:0051496 ISO
    small GTPase mediated signal transduction GO:0007264 IEA
    cell division GO:0051301 IEA
    cell morphogenesis GO:0000902 IGI
    negative regulation of cell death GO:0060548 ISO
    positive regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043280 ISO
    positive regulation of cytokinesis GO:0032467 ISO
    Rho protein signal transduction GO:0007266 TAS
    cerebral cortex cell migration GO:0021795 IMP
    stress-activated protein kinase signaling cascade GO:0031098 ISO
    regulation of calcium ion transport GO:0051924 ISO
    GTP metabolic process GO:0046039 ISO
    cell differentiation GO:0030154 IDA
    negative regulation of neuron apoptotic process GO:0043524 IMP
    negative chemotaxis GO:0050919 ISO
    positive regulation of smooth muscle contraction GO:0045987 ISO
    cytoskeleton organization GO:0007010 IMP
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 ISO
    regulation of dendrite development GO:0050773 ISO
    positive regulation of actin filament polymerization GO:0030838 ISO
    mitotic spindle assembly GO:0090307 ISO
    skeletal muscle tissue development GO:0007519 IDA
    apolipoprotein A-I-mediated signaling pathway GO:0038027 ISO
    positive regulation of podosome assembly GO:0071803 IGI
    positive regulation of lipase activity GO:0060193 ISO
    regulation of transcription from RNA polymerase II promoter GO:0006357 IDA
    positive regulation of cell growth GO:0030307 ISO
    cleavage furrow formation GO:0036089 ISO
    response to drug GO:0042493 ISO
    negative regulation of intracellular steroid hormone receptor signaling pathway GO:0033144 IDA
    ossification involved in bone maturation GO:0043931 IMP
    cell-matrix adhesion GO:0007160 IDA
    regulation of neural precursor cell proliferation GO:2000177 IMP
    integrin-mediated signaling pathway GO:0007229 TAS
Subcellular Localization
    endosome GO:0005768 ISO
    plasma membrane GO:0005886 IDA
    nucleus GO:0005634 IDA
    lamellipodium GO:0030027 IDA
    axon GO:0030424 ISO
    cytoplasm GO:0005737 ISO
    extracellular exosome GO:0070062 ISO
    cytoskeleton GO:0005856 IEA
    vesicle GO:0031982 ISO
    ruffle membrane GO:0032587 IDA
    intracellular GO:0005622 IEA
    cytosol GO:0005829 TAS
    cell cortex GO:0005938 ISO
    apical junction complex GO:0043296 ISO
    membrane GO:0016020 ISO
    mitochondrion GO:0005739 IDA
    cell projection GO:0042995 IEA
    focal adhesion GO:0005925 ISO
 Experiment description of studies that identified Rhoa in exosomes
1
Experiment ID 89
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M.
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rhoa
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGFBR1 7046
Affinity Capture-Luminescence Homo sapiens
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