Experiment description of studies that identified Msn in exosomes

1 Experiment ID 907 MISEV standards ✔ EM EV Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a EV Enriched markers ✔ HSPA5 EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S. Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 73 MISEV standards ✔ EM EV Biophysical techniques ✔ HSP70|HSP90 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 18494037     Organism Mus musculus Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes. Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ Journal name ELEC Publication year 2008 Sample Embryonic fibroblasts Sample name NIH3T3 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LCQ DECA] Western blotting

3 Experiment ID 210 MISEV standards ✔ EM EV Biophysical techniques ✔ CD81|FLOT1 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23260141     Organism Mus musculus Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL. Journal name Cell Publication year 2012 Sample Fibroblasts Sample name Normal-Fibroblasts (L cells) Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 214 MISEV standards ✔ EM EV Biophysical techniques ✘ EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23658846     Organism Mus musculus Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. Authors Hassani K, Olivier M. Journal name PLoS Negl Trop Dis Publication year 2013 Sample Macrophages Sample name Leishmania-infected-Macrophage (J774A.1) Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 215 MISEV standards ✔ EM EV Biophysical techniques ✘ EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23658846     Organism Mus musculus Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. Authors Hassani K, Olivier M. Journal name PLoS Negl Trop Dis Publication year 2013 Sample Macrophages Sample name LPS-treated-Macrophage (J774A.1) Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 216 MISEV standards ✔ EM EV Biophysical techniques ✘ EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23658846     Organism Mus musculus Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. Authors Hassani K, Olivier M. Journal name PLoS Negl Trop Dis Publication year 2013 Sample Macrophages Sample name Normal-Macrophage (J774A.1) Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 15 MISEV standards ✔ EM EV Biophysical techniques ✔ CD63 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

8 Experiment ID 14 MISEV standards ✔ EM EV Biophysical techniques ✔ RAB7|RAB11|CD9|CD63|LAMP1|LAMP2 EV Enriched markers ✔ DNM EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 16081791     Organism Mus musculus Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L Journal name JIMMU Publication year 2005 Sample Microglia Sample name N9 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LCQ DECA XP] Western blotting

9 Experiment ID 39 MISEV standards ✔ IEM EV Biophysical techniques ✔ TSG101|HSC70|FLOT1 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 15210972     Organism Mus musculus Experiment description Cells release prions in association with exosomes. Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G Journal name PNAS Publication year 2004 Sample Mov neuroglial cells Sample name Mov neuroglial cell Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy

10 Experiment ID 263 MISEV standards ✘ EV Biophysical techniques ✔ CD63|CD9 EV Enriched markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25242146     Organism Mus musculus Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino Journal name Molecular Cell Publication year 2014 Sample Neural stem cells Sample name NPCs - Th1 treated Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.20 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

11 Experiment ID 908 MISEV standards ✔ EM EV Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a EV Enriched markers ✔ HSPA5 EV Negative markers ✔ NTA EV Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S. Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Msn

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Tsc1   Two-hybrid Mus musculus View the network

Pathways in which Msn is involved

No pathways found