Gene description for TCEB1
Gene name transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
Gene symbol TCEB1
Other names/aliases SIII
eloC
Species Homo sapiens
 Database cross references - TCEB1
ExoCarta ExoCarta_6921
Entrez Gene 6921
HGNC 11617
MIM 600788
UniProt Q15369  
 TCEB1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for TCEB1
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin-protein transferase activity GO:0004842 IBA
Biological Process
    regulation of transcription from RNA polymerase II promoter in response to hypoxia GO:0061418 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    cellular response to hypoxia GO:0071456 TAS
    positive regulation of transcription elongation from RNA polymerase II promoter GO:0032968 IBA
    viral process GO:0016032 TAS
    gene expression GO:0010467 TAS
    positive regulation of viral transcription GO:0050434 TAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    elongin complex GO:0070449 IBA
 Experiment description of studies that identified TCEB1 in exosomes
1
Experiment ID 20
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
CD63|CD81|LAMP1
EV Membrane markers
✔
GOLGA2|cytochrome c
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for TCEB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SOCS1  
Affinity Capture-Western Homo sapiens
2 TCEB2 6923
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
3 TCEB3 6924
Invitro Homo sapiens
4 SOCS6  
Invitro Homo sapiens
5 ASB8  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
6 LRRC41  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
7 EXT2 2132
Affinity Capture-MS Homo sapiens
8 GC20  
Affinity Capture-MS Homo sapiens
9 VHL  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
10 USP33  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
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