Gene description for ASB1
Gene name ankyrin repeat and SOCS box containing 1
Gene symbol ASB1
Other names/aliases ASB-1
Species Homo sapiens
 Database cross references - ASB1
ExoCarta ExoCarta_51665
Vesiclepedia VP_51665
Entrez Gene 51665
HGNC 16011
MIM 605758
UniProt Q9Y576  
 ASB1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for ASB1
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin protein ligase activity GO:0061630 IDA
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 IBA
Biological Process
    negative regulation of cytokine production GO:0001818 NAS
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 IEA
    male genitalia development GO:0030539 IEA
    intracellular signal transduction GO:0035556 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IBA
    ubiquitin ligase complex GO:0000151 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ASB1 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ASB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SSR4 6748
Affinity Capture-MS Homo sapiens
2 PPP1R12B 4660
Affinity Capture-MS Homo sapiens
3 ASB5  
Affinity Capture-MS Homo sapiens
4 ARL1 400
Affinity Capture-MS Homo sapiens
5 TSHB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TCEB1 6921
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 CPS1 1373
Affinity Capture-MS Homo sapiens
8 HMGB1 3146
Affinity Capture-MS Homo sapiens
9 TRMT10C 54931
Affinity Capture-MS Homo sapiens
10 PFN1 5216
Affinity Capture-MS Homo sapiens
11 BSG 682
Affinity Capture-MS Homo sapiens
12 CUL5 8065
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 HSD17B10 3028
Affinity Capture-MS Homo sapiens
14 CAND1 55832
Affinity Capture-MS Homo sapiens
15 ATP2A2 488
Affinity Capture-MS Homo sapiens
16 SSBP1 6742
Affinity Capture-MS Homo sapiens
17 RNF7  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
18 CABP2  
Affinity Capture-MS Homo sapiens
19 APEX1 328
Affinity Capture-RNA Homo sapiens
20 EFTUD2 9343
Affinity Capture-MS Homo sapiens
21 SNRPD1 6632
Affinity Capture-MS Homo sapiens
22 TCEB2 6923
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PTS  
Affinity Capture-MS Homo sapiens
24 CASQ2 845
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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