Gene description for PPP1R12B
Gene name protein phosphatase 1, regulatory subunit 12B
Gene symbol PPP1R12B
Other names/aliases MYPT2
PP1bp55
Species Homo sapiens
 Database cross references - PPP1R12B
ExoCarta ExoCarta_4660
Vesiclepedia VP_4660
Entrez Gene 4660
HGNC 7619
MIM 603768
UniProt O60237  
 PPP1R12B identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25844599    
 Gene ontology annotations for PPP1R12B
Molecular Function
    enzyme activator activity GO:0008047 TAS
    phosphatase regulator activity GO:0019208 IEA
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of catalytic activity GO:0043085 TAS
    regulation of muscle contraction GO:0006937 TAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    signal transduction GO:0007165 TAS
    mitotic cell cycle GO:0000278 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified PPP1R12B in exosomes
1
Experiment ID 274
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
EV Enriched markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for PPP1R12B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSPA8 3312
Affinity Capture-Western Homo sapiens
2 IL16  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 PPP1CB 5500
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 PRKG1 5592
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PPP1R12B is involved
PathwayEvidenceSource
RHO GTPases activate PAKs TAS Reactome
RHO GTPases activate PKNs TAS Reactome
RHO GTPases Activate ROCKs TAS Reactome





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