Gene description for PPP1CB
Gene name protein phosphatase 1, catalytic subunit, beta isozyme
Gene symbol PPP1CB
Other names/aliases HEL-S-80p
PP-1B
PP1B
PP1beta
PPP1CD
Species Homo sapiens
 Database cross references - PPP1CB
ExoCarta ExoCarta_5500
Entrez Gene 5500
HGNC 9282
MIM 600590
UniProt P62140  
 PPP1CB identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Saliva 19199708    
Thymus 23844026    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for PPP1CB
Molecular Function
    myosin phosphatase activity GO:0017018 ISS
    metal ion binding GO:0046872 IEA
    phosphatase activity GO:0016791 ISS
    protein binding GO:0005515 IPI
    myosin-light-chain-phosphatase activity GO:0050115 IDA
    protein kinase binding GO:0019901 IPI
Biological Process
    triglyceride catabolic process GO:0019433 TAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    entrainment of circadian clock by photoperiod GO:0043153 ISS
    regulation of glycogen biosynthetic process GO:0005979 IEA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 TAS
    glycogen metabolic process GO:0005977 IEA
    mitotic cell cycle GO:0000278 TAS
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
    regulation of cell adhesion GO:0030155 IDA
    small molecule metabolic process GO:0044281 TAS
    protein dephosphorylation GO:0006470 ISS
    circadian regulation of gene expression GO:0032922 ISS
    small GTPase mediated signal transduction GO:0007264 TAS
    regulation of circadian rhythm GO:0042752 IMP
    regulation of glycogen catabolic process GO:0005981 IEA
    cell division GO:0051301 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    PTW/PP1 phosphatase complex GO:0072357 IDA
    protein phosphatase type 1 complex GO:0000164 IEA
    extracellular exosome GO:0070062 IDA
    nucleolus GO:0005730 IEA
    focal adhesion GO:0005925 IDA
    cytosol GO:0005829 TAS
    MLL5-L complex GO:0070688 IDA
    glycogen granule GO:0042587 IEA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified PPP1CB in exosomes
1
Experiment ID 76
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|GAPDH|HSP90
EV Cytosolic markers
✔
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
4
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
8
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPP1CB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMARCB1 6598
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Phenotypic Enhancement Homo sapiens
2 PPP1R12B 4660
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 RYR2 6262
Co-fractionation Homo sapiens
4 AKAP11 11215
Affinity Capture-Western Homo sapiens
5 PPP1R12A  
Affinity Capture-MS Homo sapiens
6 PPP1R7 5510
Affinity Capture-MS Homo sapiens
7 PPP1R9B  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 SH2D4A  
Affinity Capture-MS Homo sapiens
9 PPP1R12C  
Affinity Capture-MS Homo sapiens
10 PPP1R2  
Affinity Capture-MS Homo sapiens
11 NCL 4691
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 ZFYVE9  
Affinity Capture-MS Homo sapiens
13 TMEM33 55161
Affinity Capture-MS Homo sapiens
14 PPP1R11  
Affinity Capture-MS Homo sapiens
15 PPP1R8 5511
Affinity Capture-MS Homo sapiens
16 PTK2 5747
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
17 PPP1R15A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Phenotypic Suppression Homo sapiens
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