Gene description for CDC34
Gene name cell division cycle 34
Gene symbol CDC34
Other names/aliases E2-CDC34
UBC3
UBCH3
UBE2R1
Species Homo sapiens
 Database cross references - CDC34
ExoCarta ExoCarta_997
Vesiclepedia VP_997
Entrez Gene 997
HGNC 1734
MIM 116948
UniProt P49427  
 CDC34 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CDC34
Molecular Function
    ligase activity GO:0016874 IEA
    ubiquitin conjugating enzyme activity GO:0061631 IDA
    ubiquitin protein ligase binding GO:0031625 IBA
    ubiquitin-protein transferase activity GO:0004842 NAS
    protein binding GO:0005515 IPI
    ubiquitin protein ligase activity GO:0061630 IBA
    ATP binding GO:0005524 IEA
Biological Process
    positive regulation of neuron apoptotic process GO:0043525 IEA
    negative regulation of cAMP-mediated signaling GO:0043951 IDA
    G1/S transition of mitotic cell cycle GO:0000082 NAS
    DNA replication initiation GO:0006270 NAS
    response to growth factor GO:0070848 IEA
    protein K48-linked ubiquitination GO:0070936 IDA
    protein polyubiquitination GO:0000209 IDA
    positive regulation of inclusion body assembly GO:0090261 IEA
    cellular protein modification process GO:0006464 NAS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    protein ubiquitination GO:0016567 NAS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 NAS
 Experiment description of studies that identified CDC34 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CDC34
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MYBL2 4605
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
2 BTRC 8945
Reconstituted Complex Homo sapiens
3 SKP2  
Affinity Capture-MS Homo sapiens
4 CSNK2B 1460
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
5 RBX1 9978
Reconstituted Complex Homo sapiens
6 CREM  
Affinity Capture-MS Homo sapiens
7 CDK9 1025
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which CDC34 is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome
CLEC7A (Dectin-1) signaling TAS Reactome





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