Gene description for PPARG
Gene name peroxisome proliferator-activated receptor gamma
Gene symbol PPARG
Other names/aliases CIMT1
GLM1
NR1C3
PPARG1
PPARG2
PPARgamma
Species Homo sapiens
 Database cross references - PPARG
ExoCarta ExoCarta_5468
Vesiclepedia VP_5468
Entrez Gene 5468
HGNC 9236
MIM 601487
UniProt P37231  
 PPARG identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Plasma 19028452    
 Gene ontology annotations for PPARG
Molecular Function
    estrogen receptor binding GO:0030331 IEA
    protein phosphatase binding GO:0019903 IEA
    drug binding GO:0008144 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 ISS
    transcription regulatory region DNA binding GO:0044212 ISS
    DNA binding GO:0003677 ISS
    ligand-dependent nuclear receptor transcription coactivator activity GO:0030374 IDA
    protein binding GO:0005515 IPI
    retinoid X receptor binding GO:0046965 IDA
    prostaglandin receptor activity GO:0004955 TAS
    arachidonic acid binding GO:0050544 ISS
    enzyme binding GO:0019899 IPI
    steroid hormone receptor activity GO:0003707 IEA
    core promoter sequence-specific DNA binding GO:0001046 ISS
    sequence-specific DNA binding GO:0043565 IDA
    activating transcription factor binding GO:0033613 IDA
    ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity GO:0004879 IDA
    RNA polymerase II regulatory region DNA binding GO:0001012 IEA
    RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001228 IEA
    chromatin binding GO:0003682 ISS
    zinc ion binding GO:0008270 IEA
    alpha-actinin binding GO:0051393 IPI
Biological Process
    response to starvation GO:0042594 IEA
    negative regulation of collagen biosynthetic process GO:0032966 IEA
    innate immune response GO:0045087 TAS
    negative regulation of acute inflammatory response GO:0002674 IEA
    negative regulation of cell growth GO:0030308 IEA
    heart development GO:0007507 IEA
    long-chain fatty acid transport GO:0015909 ISS
    response to lipid GO:0033993 ISS
    glucose homeostasis GO:0042593 IMP
    response to metformin GO:1901558 IEA
    response to low-density lipoprotein particle GO:0055098 IDA
    epithelial cell differentiation GO:0030855 ISS
    peroxisome proliferator activated receptor signaling pathway GO:0035357 IMP
    cellular response to retinoic acid GO:0071300 IEA
    negative regulation of telomerase activity GO:0051974 IEA
    regulation of cholesterol transporter activity GO:0060694 IC
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IDA
    response to diuretic GO:0036270 IEA
    rhythmic process GO:0048511 IEA
    negative regulation of cholesterol storage GO:0010887 IDA
    positive regulation of phagocytosis, engulfment GO:0060100 IEA
    monocyte differentiation GO:0030224 IDA
    response to cold GO:0009409 IEA
    response to retinoic acid GO:0032526 IDA
    cell maturation GO:0048469 IDA
    negative regulation of transcription, DNA-templated GO:0045892 ISS
    positive regulation of transcription, DNA-templated GO:0045893 ISS
    fatty acid oxidation GO:0019395 IEA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    positive regulation of fatty acid oxidation GO:0046321 IEA
    negative regulation of interferon-gamma-mediated signaling pathway GO:0060336 IMP
    response to immobilization stress GO:0035902 IEA
    white fat cell differentiation GO:0050872 TAS
    positive regulation of oligodendrocyte differentiation GO:0048714 IEA
    cellular response to vitamin E GO:0071306 IEA
    organ regeneration GO:0031100 IEA
    response to caffeine GO:0031000 IEA
    gene expression GO:0010467 TAS
    regulation of blood pressure GO:0008217 IMP
    cellular response to lithium ion GO:0071285 IEA
    negative regulation of sequestering of triglyceride GO:0010891 IDA
    positive regulation of fat cell differentiation GO:0045600 ISS
    lipoprotein transport GO:0042953 IDA
    placenta development GO:0001890 ISS
    lipid metabolic process GO:0006629 TAS
    regulation of circadian rhythm GO:0042752 ISS
    response to mechanical stimulus GO:0009612 IEA
    steroid hormone mediated signaling pathway GO:0043401 IEA
    response to nutrient GO:0007584 TAS
    cellular response to hyperoxia GO:0071455 IEA
    cell fate commitment GO:0045165 ISS
    negative regulation of pancreatic stellate cell proliferation GO:2000230 IEA
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 IDA
    negative regulation of smooth muscle cell proliferation GO:0048662 IDA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISS
    negative regulation of receptor biosynthetic process GO:0010871 IDA
    response to vitamin A GO:0033189 IEA
    brown fat cell differentiation GO:0050873 IEA
    lipid homeostasis GO:0055088 TAS
    G-protein coupled receptor signaling pathway GO:0007186 TAS
    cellular response to insulin stimulus GO:0032869 IMP
    negative regulation of macrophage derived foam cell differentiation GO:0010745 IDA
    cellular response to prostaglandin E stimulus GO:0071380 IEA
    response to estrogen GO:0043627 IEA
    low-density lipoprotein particle receptor biosynthetic process GO:0045713 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    signal transduction GO:0007165 IDA
    regulation of transcription involved in cell fate commitment GO:0060850 ISS
Subcellular Localization
    RNA polymerase II transcription factor complex GO:0090575 IDA
    cytosol GO:0005829 ISS
    perinuclear region of cytoplasm GO:0048471 IEA
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 IDA
 Experiment description of studies that identified PPARG in exosomes
1
Experiment ID 235
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 44
MISEV standards
IEM
EV Biophysical techniques
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Immunoelectron Microscopy
Mass spectrometry
Western blotting
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for PPARG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NCOA6  
Two-hybrid Homo sapiens
Invitro Homo sapiens
2 HDAC4  
Reconstituted Complex Homo sapiens
3 Gadd45b  
Reconstituted Complex Mus musculus
4 GADD45B  
Invitro Homo sapiens
5 SVIL 6840
Two-hybrid Homo sapiens
6 MED1 5469
Invivo Homo sapiens
Invitro Homo sapiens
7 GADD45G  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
8 RB1 5925
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 PPARG 5468
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
10 BRD8 10902
Affinity Capture-MS Homo sapiens
11 NCOA2 10499
Invitro Homo sapiens
Two-hybrid Homo sapiens
12 POU1F1  
Reconstituted Complex Homo sapiens
13 FABP1 2168
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
14 CREBBP  
Reconstituted Complex Homo sapiens
15 DUT 1854
Affinity Capture-MS Homo sapiens
16 NR0B2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 HDAC3 8841
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
18 MED24 9862
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
19 RXRA 6256
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
20 RXRG  
Affinity Capture-MS Homo sapiens
21 NFE2L2 4780
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
22 NRIP1 8204
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
23 EDF1 8721
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
24 PPARGC1A  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
25 EP300 2033
Reconstituted Complex Homo sapiens
26 NCOA4 8031
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
27 ERBP  
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
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