Gene description for MED1
Gene name mediator complex subunit 1
Gene symbol MED1
Other names/aliases CRSP1
CRSP200
DRIP205
DRIP230
PBP
PPARBP
PPARGBP
RB18A
TRAP220
TRIP2
Species Homo sapiens
 Database cross references - MED1
ExoCarta ExoCarta_5469
Vesiclepedia VP_5469
Entrez Gene 5469
HGNC 9234
MIM 604311
UniProt Q15648  
 MED1 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MED1
Molecular Function
    sequence-specific DNA binding RNA polymerase II transcription factor activity GO:0000981 ISS
    RNA polymerase II transcription cofactor activity GO:0001104 IDA
    thyroid hormone receptor binding GO:0046966 IPI
    vitamin D receptor binding GO:0042809 TAS
    ligand-dependent nuclear receptor binding GO:0016922 IPI
    chromatin DNA binding GO:0031490 IEA
    core promoter binding GO:0001047 IDA
    ligand-dependent nuclear receptor transcription coactivator activity GO:0030374 NAS
    receptor activity GO:0004872 IDA
    LBD domain binding GO:0050693 IPI
    protein binding GO:0005515 IPI
    transcription factor binding GO:0008134 IPI
    transcription coactivator activity GO:0003713 IMP
    mediator complex binding GO:0036033 IDA
    chromatin binding GO:0003682 IMP
    retinoic acid receptor binding GO:0042974 IPI
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IEA
    nuclear hormone receptor binding GO:0035257 IPI
    estrogen receptor binding GO:0030331 IPI
    peroxisome proliferator activated receptor binding GO:0042975 IPI
    transcription cofactor activity GO:0003712 IDA
    thyroid hormone receptor coactivator activity GO:0030375 IMP
Biological Process
    peroxisome proliferator activated receptor signaling pathway GO:0035357 IEA
    cellular response to hepatocyte growth factor stimulus GO:0035729 IEA
    erythrocyte development GO:0048821 ISS
    ERK1 and ERK2 cascade GO:0070371 IDA
    cellular lipid metabolic process GO:0044255 TAS
    mammary gland branching involved in thelarche GO:0060744 IEA
    lactation GO:0007595 IEA
    mRNA transcription from RNA polymerase II promoter GO:0042789 ISS
    androgen receptor signaling pathway GO:0030521 IDA
    positive regulation of keratinocyte differentiation GO:0045618 IMP
    intracellular steroid hormone receptor signaling pathway GO:0030518 IDA
    negative regulation of apoptotic process GO:0043066 ISS
    androgen biosynthetic process GO:0006702 IMP
    ventricular trabecula myocardium morphogenesis GO:0003222 IEA
    positive regulation of hepatocyte proliferation GO:2000347 IEA
    organ regeneration GO:0031100 IEA
    positive regulation of G0 to G1 transition GO:0070318 IEA
    embryonic placenta development GO:0001892 IEA
    retinal pigment epithelium development GO:0003406 IEA
    small molecule metabolic process GO:0044281 TAS
    liver development GO:0001889 IEA
    positive regulation of protein import into nucleus, translocation GO:0033160 IEA
    regulation of vitamin D receptor signaling pathway GO:0070562 IEA
    gene expression GO:0010467 TAS
    positive regulation of mammary gland epithelial cell proliferation GO:0033601 IEA
    regulation of cell cycle GO:0051726 NAS
    epithelial cell proliferation involved in mammary gland duct elongation GO:0060750 IEA
    positive regulation of receptor activity GO:2000273 IMP
    regulation of transcription from RNA polymerase I promoter GO:0006356 IDA
    embryonic heart tube development GO:0035050 IEA
    lens development in camera-type eye GO:0002088 ISS
    enucleate erythrocyte development GO:0048822 IEA
    angiogenesis GO:0001525 ISS
    thyroid hormone generation GO:0006590 IEA
    negative regulation of keratinocyte proliferation GO:0010839 IMP
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    negative regulation of neuron differentiation GO:0045665 ISS
    embryonic hemopoiesis GO:0035162 IEA
    positive regulation of intracellular estrogen receptor signaling pathway GO:0033148 IEA
    thyroid hormone mediated signaling pathway GO:0002154 IMP
    cellular response to steroid hormone stimulus GO:0071383 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    fat cell differentiation GO:0045444 IDA
    brain development GO:0007420 IEA
    embryonic hindlimb morphogenesis GO:0035116 IEA
    positive regulation of gene expression GO:0010628 IMP
    monocyte differentiation GO:0030224 IEA
    megakaryocyte development GO:0035855 ISS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISS
    keratinocyte differentiation GO:0030216 IMP
    mammary gland branching involved in pregnancy GO:0060745 IEA
    positive regulation of interferon-gamma-mediated signaling pathway GO:0060335 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    cell morphogenesis GO:0000902 IMP
    cellular response to thyroid hormone stimulus GO:0097067 IDA
    regulation of RNA biosynthetic process GO:2001141 IMP
    cellular response to epidermal growth factor stimulus GO:0071364 IDA
Subcellular Localization
    mediator complex GO:0016592 IMP
    nucleolus GO:0005730 IDA
    chromatin GO:0000785 IEA
    nucleus GO:0005634 IDA
    membrane GO:0016020 IDA
    protein-DNA complex GO:0032993 IEA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified MED1 in exosomes
1
Experiment ID 497
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MED1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MED19  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 VDR  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 RARA 5914
Invivo Homo sapiens
Invitro Homo sapiens
4 IXL  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 CDK8 1024
Affinity Capture-MS Homo sapiens
7 THRB 7068
Invivo Homo sapiens
Invitro Homo sapiens
8 MED1 5469
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
9 PPARG 5468
Invivo Homo sapiens
Invitro Homo sapiens
10 MED9  
Affinity Capture-MS Homo sapiens
11 TRG20  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 NR3C1 2908
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
13 BRCA1 672
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
14 ESR1  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 HNF4A  
Invivo Homo sapiens
Invitro Homo sapiens
16 ESR2  
Invivo Homo sapiens
Invitro Homo sapiens
17 PPARA  
Invivo Homo sapiens
Invitro Homo sapiens
18 RXRA 6256
Invivo Homo sapiens
Invitro Homo sapiens
19 CRSP7  
Affinity Capture-MS Homo sapiens
20 NCOA6IP  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 YWHAQ 10971
Reconstituted Complex Homo sapiens
22 THRA  
Invitro Homo sapiens
Invivo Homo sapiens
23 MED25  
Affinity Capture-Western Homo sapiens
24 AR 367
Invivo Homo sapiens
Invitro Homo sapiens
25 PPARGC1A  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
26 Thrb  
Two-hybrid Rattus norvegicus
27 MED28  
Affinity Capture-MS Homo sapiens
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