Gene description for TRRAP
Gene name
transformation/transcription domain-associated protein
Gene symbol
TRRAP
Other names/aliases
PAF350/400 PAF400 STAF40 TR-AP Tra1
Species
Homo sapiens
Database cross references - TRRAP
ExoCarta
ExoCarta_8295
Entrez Gene
8295
HGNC
12347
MIM
603015
UniProt
Q9Y4A5
TRRAP identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells
26054723
Thymus
23844026
Gene ontology annotations for TRRAP
Experiment description of studies that identified TRRAP in exosomes
1
Experiment ID
235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
2
Experiment ID
217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for TRRAP
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
TAL1
Affinity Capture-MS
Homo sapiens
2
ESR1
Affinity Capture-MS
Homo sapiens
3
E2F1
1869
Affinity Capture-Western
Homo sapiens
4
GCN5L2
Affinity Capture-Western
Homo sapiens
5
Myc
Affinity Capture-Western
Mus musculus
6
VDR
Affinity Capture-MS
Homo sapiens
7
MYC
Affinity Capture-Western
Homo sapiens
Co-purification
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
8
ING1
Affinity Capture-Western
Homo sapiens
9
RUVBL1
8607
Affinity Capture-Western
Homo sapiens
10
PCAF
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
11
ACTL6A
86
Co-fractionation
Homo sapiens
Affinity Capture-Western
Homo sapiens
12
MAX
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
13
EP400
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
View the network
image/svg+xml
Pathways in which TRRAP is involved