Gene description for TRRAP
Gene name transformation/transcription domain-associated protein
Gene symbol TRRAP
Other names/aliases PAF350/400
PAF400
STAF40
TR-AP
Tra1
Species Homo sapiens
 Database cross references - TRRAP
ExoCarta ExoCarta_8295
Entrez Gene 8295
HGNC 12347
MIM 603015
UniProt Q9Y4A5  
 TRRAP identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
 Gene ontology annotations for TRRAP
Molecular Function
    protein binding GO:0005515 IPI
    transcription coactivator activity GO:0003713 IEA
    transcription cofactor activity GO:0003712 IDA
    phosphotransferase activity, alcohol group as acceptor GO:0016773 IEA
    kinase activity GO:0016301 IBA
Biological Process
    chromatin organization GO:0006325 TAS
    transcription, DNA-templated GO:0006351 IEA
    histone H2A acetylation GO:0043968 IDA
    DNA repair GO:0006281 IBA
    histone H4 acetylation GO:0043967 IDA
    histone deubiquitination GO:0016578 IDA
    phosphorylation GO:0016310 IBA
    histone acetylation GO:0016573 NAS
    mitotic cell cycle checkpoint GO:0007093 IEA
    regulation of transcription, DNA-templated GO:0006355 IEA
Subcellular Localization
    STAGA complex GO:0030914 IDA
    nucleoplasm GO:0005654 TAS
    Swr1 complex GO:0000812 IDA
    NuA4 histone acetyltransferase complex GO:0035267 IDA
    transcription factor TFTC complex GO:0033276 IDA
    PCAF complex GO:0000125 NAS
    nucleus GO:0005634 IDA
    Golgi apparatus GO:0005794 IDA
 Experiment description of studies that identified TRRAP in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRRAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TAL1  
Affinity Capture-MS Homo sapiens
2 ESR1  
Affinity Capture-MS Homo sapiens
3 E2F1 1869
Affinity Capture-Western Homo sapiens
4 GCN5L2  
Affinity Capture-Western Homo sapiens
5 Myc  
Affinity Capture-Western Mus musculus
6 VDR  
Affinity Capture-MS Homo sapiens
7 MYC  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 ING1  
Affinity Capture-Western Homo sapiens
9 RUVBL1 8607
Affinity Capture-Western Homo sapiens
10 PCAF  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 ACTL6A 86
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
12 MAX  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 EP400  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which TRRAP is involved
PathwayEvidenceSource
formation of the beta-catenin:TCF transactivating complex TAS Reactome
HATs acetylate histones TAS Reactome





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