Gene description for DOT1L
Gene name DOT1-like histone H3K79 methyltransferase
Gene symbol DOT1L
Other names/aliases DOT1
KMT4
Species Homo sapiens
 Database cross references - DOT1L
ExoCarta ExoCarta_84444
Entrez Gene 84444
HGNC 24948
MIM 607375
UniProt Q8TEK3  
 DOT1L identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for DOT1L
Molecular Function
    histone methyltransferase activity (H3-K79 specific) GO:0031151 IEA
    transcription factor binding GO:0008134 IPI
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    histone-lysine N-methyltransferase activity GO:0018024 IDA
Biological Process
    regulation of transcription regulatory region DNA binding GO:2000677 IMP
    histone H3-K79 methylation GO:0034729 IEA
    chromatin silencing GO:0006342 IEA
    chromatin organization GO:0006325 TAS
    regulation of JAK-STAT cascade GO:0046425 IDA
    regulation of cell cycle GO:0051726 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified DOT1L in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for DOT1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DOT1L is involved
PathwayEvidenceSource
PKMTs methylate histone lysines TAS Reactome





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