Gene description for GRWD1
Gene name glutamate-rich WD repeat containing 1
Gene symbol GRWD1
Other names/aliases CDW4
GRWD
RRB1
WDR28
Species Homo sapiens
 Database cross references - GRWD1
ExoCarta ExoCarta_83743
Vesiclepedia VP_83743
Entrez Gene 83743
HGNC 21270
MIM 610597
UniProt Q9BQ67  
 GRWD1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for GRWD1
Molecular Function
    chromatin binding GO:0003682 IDA
    DNA replication origin binding GO:0003688 IDA
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    histone binding GO:0042393 IDA
Biological Process
    DNA replication GO:0006260 IMP
    nucleosome assembly GO:0006334 IDA
    nucleosome assembly GO:0006334 IMP
    nucleosome disassembly GO:0006337 IMP
    ribosome biogenesis GO:0042254 IBA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    chromosome GO:0005694 IEA
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 IDA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified GRWD1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
13
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GRWD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 Mdm2  
Affinity Capture-MS Mus musculus
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 COIL  
Proximity Label-MS Homo sapiens
5 IPO7 10527
Affinity Capture-MS Homo sapiens
6 SPTLC1 10558
Affinity Capture-MS Homo sapiens
7 UBC 7316
Affinity Capture-MS Homo sapiens
8 VHL  
Affinity Capture-MS Homo sapiens
9 CAND1 55832
Affinity Capture-MS Homo sapiens
10 KMT2A  
Affinity Capture-Western Homo sapiens
11 WDR36 134430
Co-fractionation Homo sapiens
12 USP36  
Affinity Capture-MS Homo sapiens
13 DIMT1 27292
Co-fractionation Homo sapiens
14 KIF23 9493
Affinity Capture-MS Homo sapiens
15 DDX24 57062
Co-fractionation Homo sapiens
16 COPS5 10987
Affinity Capture-MS Homo sapiens
17 NPM1 4869
Affinity Capture-MS Homo sapiens
18 KMT2D 8085
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 POLR1E  
Co-fractionation Homo sapiens
20 CDT1  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
21 DDX18 8886
Co-fractionation Homo sapiens
22 ATG16L1 55054
Affinity Capture-MS Homo sapiens
23 CEP57  
Affinity Capture-MS Homo sapiens
24 METTL18  
Affinity Capture-MS Homo sapiens
25 CUL1 8454
Affinity Capture-MS Homo sapiens
26 ARIH2 10425
Affinity Capture-MS Homo sapiens
27 WDR5 11091
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 KIF14 9928
Affinity Capture-MS Homo sapiens
29 FAS 355
Proximity Label-MS Homo sapiens
30 CHMP4C 92421
Affinity Capture-MS Homo sapiens
31 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
32 USP11 8237
Affinity Capture-MS Homo sapiens
33 CUL4B 8450
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
34 PARK2  
Affinity Capture-MS Homo sapiens
35 DOT1L 84444
Affinity Capture-MS Homo sapiens
36 CCNB1 891
Affinity Capture-MS Homo sapiens
37 NOC2L 26155
Co-fractionation Homo sapiens
38 CHMP4B 128866
Affinity Capture-MS Homo sapiens
39 VCP 7415
Affinity Capture-MS Homo sapiens
40 OGT 8473
Reconstituted Complex Homo sapiens
41 IFI16 3428
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 NCL 4691
Affinity Capture-MS Homo sapiens
43 TRIM11  
Affinity Capture-MS Homo sapiens
44 HECTD1 25831
Affinity Capture-MS Homo sapiens
45 CEP76  
Affinity Capture-MS Homo sapiens
46 PPM1G 5496
Affinity Capture-MS Homo sapiens
47 CEBPA  
Protein-peptide Homo sapiens
48 GTPBP4 23560
Co-fractionation Homo sapiens
49 RPL11 6135
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
50 ANLN 54443
Affinity Capture-MS Homo sapiens
51 ZBTB2 57621
Affinity Capture-MS Homo sapiens
52 HIST1H3A 8350
Affinity Capture-Western Homo sapiens
53 NR4A1  
Affinity Capture-MS Homo sapiens
54 FBXL7  
Affinity Capture-MS Homo sapiens
55 ATL3 25923
Affinity Capture-MS Homo sapiens
56 XPO1 7514
Affinity Capture-MS Homo sapiens
57 RC3H2  
Affinity Capture-MS Homo sapiens
58 SEC13 6396
Affinity Capture-MS Homo sapiens
59 DNAJC2 27000
Affinity Capture-MS Homo sapiens
60 KMT2E  
Affinity Capture-MS Homo sapiens
61 PRKCZ 5590
Affinity Capture-MS Homo sapiens
62 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 DCAF13 25879
Affinity Capture-MS Homo sapiens
64 RPL3 6122
Affinity Capture-MS Homo sapiens
65 NXF1 10482
Affinity Capture-RNA Homo sapiens
66 SETD1A 9739
Affinity Capture-Western Homo sapiens
67 TOP1 7150
Protein-peptide Homo sapiens
68 DDB1 1642
Affinity Capture-Western Homo sapiens
69 DDRGK1 65992
Affinity Capture-MS Homo sapiens
70 HUWE1 10075
Affinity Capture-MS Homo sapiens
71 GRK5 2869
Affinity Capture-MS Homo sapiens
72 C8orf33 65265
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
74 RBM19 9904
Co-fractionation Homo sapiens
75 HDGF 3068
Affinity Capture-MS Homo sapiens
76 TP53 7157
Affinity Capture-MS Homo sapiens
77 NOL10  
Co-fractionation Homo sapiens
78 MNDA 4332
Affinity Capture-MS Homo sapiens
79 MEN1 4221
Affinity Capture-MS Homo sapiens
80 ESR1  
Reconstituted Complex Homo sapiens
81 UFL1 23376
Affinity Capture-MS Homo sapiens
82 E2F4  
Affinity Capture-MS Homo sapiens
83 MOV10 4343
Affinity Capture-RNA Homo sapiens
84 C16orf72 29035
Affinity Capture-MS Homo sapiens
85 TRIM31  
Affinity Capture-MS Homo sapiens
86 BCCIP 56647
Co-fractionation Homo sapiens
87 SERBP1 26135
Affinity Capture-MS Homo sapiens
88 KRAS 3845
Proximity Label-MS Homo sapiens
89 POLR1D 51082
Co-fractionation Homo sapiens
90 RC3H1 149041
Affinity Capture-MS Homo sapiens
91 PRMT1 3276
Affinity Capture-MS Homo sapiens
92 DDX56  
Co-fractionation Homo sapiens
93 CUL4A 8451
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
94 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GRWD1 is involved
No pathways found





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