Gene description for ARIH2
Gene name ariadne RBR E3 ubiquitin protein ligase 2
Gene symbol ARIH2
Other names/aliases ARI2
TRIAD1
Species Homo sapiens
 Database cross references - ARIH2
ExoCarta ExoCarta_10425
Vesiclepedia VP_10425
Entrez Gene 10425
HGNC 690
MIM 605615
UniProt O95376  
 ARIH2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ARIH2
Molecular Function
    ligase activity GO:0016874 IEA
    zinc ion binding GO:0008270 IEA
    protein binding GO:0005515 IPI
    nucleic acid binding GO:0003676 IEA
    ubiquitin-protein transferase activity GO:0004842 IDA
Biological Process
    hematopoietic stem cell proliferation GO:0071425 IDA
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IDA
    positive regulation of protein targeting to mitochondrion GO:1903955 IMP
    multicellular organismal development GO:0007275 TAS
    protein K48-linked ubiquitination GO:0070936 IDA
    protein K63-linked ubiquitination GO:0070534 IDA
    developmental cell growth GO:0048588 IDA
    protein polyubiquitination GO:0000209 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified ARIH2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ARIH2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTN  
Two-hybrid Homo sapiens
2 SGCE 8910
Two-hybrid Homo sapiens
3 EEF1A1 1915
Two-hybrid Homo sapiens
4 UGP2 7360
Two-hybrid Homo sapiens
5 TP53 7157
Two-hybrid Homo sapiens
6 BUB1  
Two-hybrid Homo sapiens
7 UBE2L3 7332
Two-hybrid Homo sapiens
8 WDR91 29062
Two-hybrid Homo sapiens
9 ENSA 2029
Two-hybrid Homo sapiens
10 UTP14A 10813
Two-hybrid Homo sapiens
11 EML4 27436
Two-hybrid Homo sapiens
12 LOC147791  
Two-hybrid Homo sapiens
13 KIAA1377  
Two-hybrid Homo sapiens
14 SLC1A6  
Two-hybrid Homo sapiens
15 IL4R  
Two-hybrid Homo sapiens
16 HTATIP  
Two-hybrid Homo sapiens
17 CBR3 874
Two-hybrid Homo sapiens
18 PPP2R1A 5518
Two-hybrid Homo sapiens
19 SCAMP2 10066
Two-hybrid Homo sapiens
20 DLST 1743
Two-hybrid Homo sapiens
21 KCNQ2  
Two-hybrid Homo sapiens
22 RPL8 6132
Two-hybrid Homo sapiens
23 ARAP1 116985
Two-hybrid Homo sapiens
24 EEF1G 1937
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ARIH2 is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome





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