Gene description for ARIH2
Gene name ariadne RBR E3 ubiquitin protein ligase 2
Gene symbol ARIH2
Other names/aliases ARI2
TRIAD1
Species Homo sapiens
 Database cross references - ARIH2
ExoCarta ExoCarta_10425
Vesiclepedia VP_10425
Entrez Gene 10425
HGNC 690
MIM 605615
UniProt O95376  
 ARIH2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ARIH2
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    ubiquitin conjugating enzyme binding GO:0031624 IBA
    ubiquitin conjugating enzyme binding GO:0031624 IPI
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
Biological Process
    protein polyubiquitination GO:0000209 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    protein ubiquitination GO:0016567 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    developmental cell growth GO:0048588 IDA
    protein K63-linked ubiquitination GO:0070534 IDA
    protein K48-linked ubiquitination GO:0070936 IDA
    hematopoietic stem cell proliferation GO:0071425 IDA
    positive regulation of protein targeting to mitochondrion GO:1903955 HMP
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    Cul5-RING ubiquitin ligase complex GO:0031466 IDA
 Experiment description of studies that identified ARIH2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ARIH2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
2 RPL14 9045
Affinity Capture-MS Homo sapiens
3 WDR91 29062
Two-hybrid Homo sapiens
4 RPSA 3921
Affinity Capture-MS Homo sapiens
5 SGCE 8910
Two-hybrid Homo sapiens
6 EXOSC7 23016
Affinity Capture-MS Homo sapiens
7 RPS28 6234
Affinity Capture-MS Homo sapiens
8 TWF2 11344
Co-fractionation Homo sapiens
9 TCEB1 6921
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
10 KRT1 3848
Affinity Capture-MS Homo sapiens
11 EEF1G 1937
Two-hybrid Homo sapiens
12 RPS3A 6189
Affinity Capture-MS Homo sapiens
13 BUB1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 RPL30 6156
Affinity Capture-MS Homo sapiens
15 RPS17 6218
Affinity Capture-MS Homo sapiens
16 CIRBP 1153
Affinity Capture-MS Homo sapiens
17 DYNC1I2 1781
Affinity Capture-MS Homo sapiens
18 REL 5966
Two-hybrid Homo sapiens
19 HNRNPDL 9987
Affinity Capture-MS Homo sapiens
20 ALB 213
Affinity Capture-MS Homo sapiens
21 RAB40C 57799
Affinity Capture-MS Homo sapiens
22 PACSIN2 11252
Co-fractionation Homo sapiens
23 HSPA1L 3305
Affinity Capture-MS Homo sapiens
24 ARL6IP4  
Affinity Capture-MS Homo sapiens
25 SPSB4  
Affinity Capture-MS Homo sapiens
26 RPL15 6138
Affinity Capture-MS Homo sapiens
27 UBC 7316
Biochemical Activity Homo sapiens
28 SSB 6741
Affinity Capture-MS Homo sapiens
29 XRN2 22803
Affinity Capture-MS Homo sapiens
30 HNRNPCL1 343069
Affinity Capture-MS Homo sapiens
31 HNRNPR 10236
Affinity Capture-MS Homo sapiens
32 SRSF2 6427
Affinity Capture-MS Homo sapiens
33 SLC1A6  
Two-hybrid Homo sapiens
34 GFI1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
35 SNRPD2 6633
Affinity Capture-MS Homo sapiens
36 HSPA5 3309
Affinity Capture-MS Homo sapiens
37 ILF2 3608
Affinity Capture-MS Homo sapiens
38 APEX1 328
Co-fractionation Homo sapiens
39 RPS18 6222
Affinity Capture-MS Homo sapiens
40 TNRC6C  
Affinity Capture-MS Homo sapiens
41 RPL4 6124
Affinity Capture-MS Homo sapiens
42 RHEB 6009
Two-hybrid Homo sapiens
43 HNRNPAB 3182
Affinity Capture-MS Homo sapiens
44 NKRF 55922
Affinity Capture-MS Homo sapiens
45 TRIM28 10155
Affinity Capture-MS Homo sapiens
46 HIST1H2BK 85236
Affinity Capture-MS Homo sapiens
47 TNRC6A 27327
Affinity Capture-MS Homo sapiens
48 TCEB2 6923
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
49 UBE2E2  
Two-hybrid Homo sapiens
50 RBMXL2  
Affinity Capture-MS Homo sapiens
51 H1FX 8971
Affinity Capture-MS Homo sapiens
52 DAZAP1 26528
Affinity Capture-MS Homo sapiens
53 KCNQ2  
Two-hybrid Homo sapiens
54 NUDT1  
Affinity Capture-MS Homo sapiens
55 RCN2 5955
Affinity Capture-MS Homo sapiens
56 AFAP1L2 84632
Co-fractionation Homo sapiens
57 RPL24 6152
Affinity Capture-MS Homo sapiens
58 PCMTD2  
Affinity Capture-MS Homo sapiens
59 NPM1 4869
Affinity Capture-MS Homo sapiens
60 SNRPC 6631
Affinity Capture-MS Homo sapiens
61 RPS8 6202
Affinity Capture-MS Homo sapiens
62 TRAP1 10131
Affinity Capture-MS Homo sapiens
63 ASB9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 TFIP11  
Co-fractionation Homo sapiens
65 EWSR1 2130
Affinity Capture-MS Homo sapiens
66 SRSF1 6426
Affinity Capture-MS Homo sapiens
67 SRSF3 6428
Affinity Capture-MS Homo sapiens
68 RPL10 6134
Affinity Capture-MS Homo sapiens
69 TCEANC2  
Affinity Capture-MS Homo sapiens
70 GRWD1 83743
Affinity Capture-MS Homo sapiens
71 TULP3 7289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 SNRPD3 6634
Affinity Capture-MS Homo sapiens
73 KRT72 140807
Affinity Capture-MS Homo sapiens
74 TUBB 203068
Affinity Capture-MS Homo sapiens
75 PHF7  
Two-hybrid Homo sapiens
76 EXOSC6 118460
Affinity Capture-MS Homo sapiens
77 ARIH2 10425
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
78 RPL36 25873
Affinity Capture-MS Homo sapiens
79 PDLIM1 9124
Co-fractionation Homo sapiens
80 EHD1 10938
Co-fractionation Homo sapiens
81 RPS26 6231
Affinity Capture-MS Homo sapiens
82 HSPA8 3312
Affinity Capture-MS Homo sapiens
83 EXOSC9 5393
Affinity Capture-MS Homo sapiens
84 UBE2V1 7335
Two-hybrid Homo sapiens
85 BRIX1 55299
Affinity Capture-MS Homo sapiens
86 XPA  
Affinity Capture-MS Homo sapiens
87 KRT8 3856
Affinity Capture-MS Homo sapiens
88 UBE2E3 10477
Two-hybrid Homo sapiens
89 PWP1 11137
Affinity Capture-MS Homo sapiens
90 NSUN2 54888
Affinity Capture-MS Homo sapiens
91 YBX1 4904
Affinity Capture-MS Homo sapiens
92 ENSA 2029
Two-hybrid Homo sapiens
93 UTP14A 10813
Two-hybrid Homo sapiens
94 EML4 27436
Two-hybrid Homo sapiens
95 DDX21 9188
Affinity Capture-MS Homo sapiens
96 UBE2T  
Two-hybrid Homo sapiens
97 RPL9 6133
Affinity Capture-MS Homo sapiens
98 PARK2  
Affinity Capture-MS Homo sapiens
99 UBE2D4  
Two-hybrid Homo sapiens
100 SPSB2  
Affinity Capture-MS Homo sapiens
101 CCDC33 80125
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
102 RPL19 6143
Affinity Capture-MS Homo sapiens
103 STAU1 6780
Affinity Capture-MS Homo sapiens
104 NOP2 4839
Affinity Capture-MS Homo sapiens
105 SOCS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 C12orf49  
Negative Genetic Homo sapiens
107 ACACA 31
Positive Genetic Homo sapiens
108 RPL5 6125
Affinity Capture-MS Homo sapiens
109 CBR3 874
Two-hybrid Homo sapiens
110 SMARCA4 6597
Affinity Capture-MS Homo sapiens
111 PABPC1 26986
Affinity Capture-MS Homo sapiens
112 SNRPD1 6632
Affinity Capture-MS Homo sapiens
113 ZFP41  
Affinity Capture-MS Homo sapiens
114 VCP 7415
Affinity Capture-MS Homo sapiens
115 TRIM21 6737
Affinity Capture-MS Homo sapiens
116 OGT 8473
Reconstituted Complex Homo sapiens
117 SCAMP2 10066
Two-hybrid Homo sapiens
118 ACTL6A 86
Affinity Capture-MS Homo sapiens
119 KRT9 3857
Affinity Capture-MS Homo sapiens
120 U2AF2 11338
Affinity Capture-MS Homo sapiens
121 PARP6  
Co-fractionation Homo sapiens
122 LOC147791  
Two-hybrid Homo sapiens
123 RPL13A 23521
Affinity Capture-MS Homo sapiens
124 HIST2H4A 8370
Affinity Capture-MS Homo sapiens
125 KRT14 3861
Affinity Capture-MS Homo sapiens
126 MEOX2  
Two-hybrid Homo sapiens
127 APOBEC3G  
Biochemical Activity Homo sapiens
128 RPLP1 6176
Affinity Capture-MS Homo sapiens
129 TUBA1B 10376
Affinity Capture-MS Homo sapiens
130 PML 5371
Affinity Capture-Western Homo sapiens
131 PABPN1 8106
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
132 UBE2D3 7323
Two-hybrid Homo sapiens
133 DDX17 10521
Affinity Capture-MS Homo sapiens
134 RPLP0 6175
Affinity Capture-MS Homo sapiens
135 RPS16 6217
Affinity Capture-MS Homo sapiens
136 BMX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 HNRNPA3 220988
Affinity Capture-MS Homo sapiens
138 RPS15A 6210
Affinity Capture-MS Homo sapiens
139 SNRPE 6635
Affinity Capture-MS Homo sapiens
140 PAPSS2 9060
Co-fractionation Homo sapiens
141 RBX1 9978
Co-fractionation Homo sapiens
142 RPL11 6135
Affinity Capture-MS Homo sapiens
143 CBFB 865
Affinity Capture-MS Homo sapiens
144 TUBB4B 10383
Affinity Capture-MS Homo sapiens
145 RTCB 51493
Affinity Capture-MS Homo sapiens
146 PTN  
Two-hybrid Homo sapiens
147 DISC1 27185
Two-hybrid Homo sapiens
148 HNRNPD 3184
Affinity Capture-MS Homo sapiens
149 UBE2N 7334
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
150 NTRK1 4914
Affinity Capture-MS Homo sapiens
151 RBM39 9584
Affinity Capture-MS Homo sapiens
152 RPS19 6223
Affinity Capture-MS Homo sapiens
153 TAF15 8148
Affinity Capture-MS Homo sapiens
154 RPLP2 6181
Affinity Capture-MS Homo sapiens
155 RAB40B  
Affinity Capture-MS Homo sapiens
156 RPL23 9349
Affinity Capture-MS Homo sapiens
157 RPS2 6187
Affinity Capture-MS Homo sapiens
158 DHX15 1665
Affinity Capture-MS Homo sapiens
159 RPS20 6224
Affinity Capture-MS Homo sapiens
160 SNRPB 6628
Affinity Capture-MS Homo sapiens
161 UBE2L3 7332
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
162 EEF2 1938
Affinity Capture-MS Homo sapiens
163 POLR2E 5434
Affinity Capture-MS Homo sapiens
164 UBE2L6 9246
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
165 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
166 ASB6 140459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
167 QPCT 25797
Affinity Capture-MS Homo sapiens
168 CUL3 8452
Affinity Capture-MS Homo sapiens
169 RPS6 6194
Affinity Capture-MS Homo sapiens
170 SRSF5 6430
Affinity Capture-MS Homo sapiens
171 RPL3 6122
Affinity Capture-MS Homo sapiens
172 UBE2E1  
Two-hybrid Homo sapiens
173 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
174 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
175 KIAA1377  
Two-hybrid Homo sapiens
176 HNRNPF 3185
Affinity Capture-MS Homo sapiens
177 HNRNPUL1 11100
Affinity Capture-MS Homo sapiens
178 AGL 178
Affinity Capture-MS Homo sapiens
179 TNRC6B  
Affinity Capture-MS Homo sapiens
180 UBE2R2 54926
Two-hybrid Homo sapiens
181 TFG 10342
Affinity Capture-MS Homo sapiens
182 CRX  
Two-hybrid Homo sapiens
183 UBB 7314
Affinity Capture-MS Homo sapiens
184 PRMT5 10419
Affinity Capture-MS Homo sapiens
185 UBE2D2 7322
Two-hybrid Homo sapiens
186 LUC7L2 51631
Affinity Capture-MS Homo sapiens
187 APOBEC3F 200316
Biochemical Activity Homo sapiens
188 PPP2R1A 5518
Two-hybrid Homo sapiens
189 RPS13 6207
Affinity Capture-MS Homo sapiens
190 FUS 2521
Affinity Capture-MS Homo sapiens
191 DLST 1743
Two-hybrid Homo sapiens
192 RPS14 6208
Affinity Capture-MS Homo sapiens
193 TRIM27  
Two-hybrid Homo sapiens
194 RPL12 6136
Affinity Capture-MS Homo sapiens
195 KAT5  
Two-hybrid Homo sapiens
196 RPL22 6146
Affinity Capture-MS Homo sapiens
197 SART3 9733
Affinity Capture-MS Homo sapiens
198 NUBP1 4682
Co-fractionation Homo sapiens
199 DDB1 1642
Affinity Capture-MS Homo sapiens
200 WSB2 55884
Affinity Capture-MS Homo sapiens
201 RPL18 6141
Affinity Capture-MS Homo sapiens
202 SNRPG 6637
Affinity Capture-MS Homo sapiens
203 PARK7 11315
Affinity Capture-MS Homo sapiens
204 CLTC 1213
Affinity Capture-MS Homo sapiens
205 RPL21 6144
Affinity Capture-MS Homo sapiens
206 RPL7 6129
Affinity Capture-MS Homo sapiens
207 HSPA9 3313
Affinity Capture-MS Homo sapiens
208 RPS10 6204
Affinity Capture-MS Homo sapiens
209 RPL26 6154
Affinity Capture-MS Homo sapiens
210 RPS23 6228
Affinity Capture-MS Homo sapiens
211 RPL35A 6165
Affinity Capture-MS Homo sapiens
212 KRT2 3849
Affinity Capture-MS Homo sapiens
213 MDM2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
214 BAG4  
Affinity Capture-MS Homo sapiens
215 RBBP4 5928
Affinity Capture-MS Homo sapiens
216 ARAP1 116985
Two-hybrid Homo sapiens
217 DHX9 1660
Affinity Capture-MS Homo sapiens
218 UBE2D1 7321
Two-hybrid Homo sapiens
219 EEF1A1 1915
Two-hybrid Homo sapiens
220 HK2 3099
Co-fractionation Homo sapiens
221 RPL13 6137
Affinity Capture-MS Homo sapiens
222 UGP2 7360
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
223 ASB3 51130
Affinity Capture-MS Homo sapiens
224 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
225 RPS25 6230
Affinity Capture-MS Homo sapiens
226 TIA1 7072