Gene description for UBE2L6
Gene name ubiquitin-conjugating enzyme E2L 6
Gene symbol UBE2L6
Other names/aliases RIG-B
UBCH8
Species Homo sapiens
 Database cross references - UBE2L6
ExoCarta ExoCarta_9246
Vesiclepedia VP_9246
Entrez Gene 9246
HGNC 12490
MIM 603890
UniProt O14933  
 UBE2L6 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for UBE2L6
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 TAS
    protein binding GO:0005515 IPI
    ISG15 transferase activity GO:0042296 IBA
    ISG15 transferase activity GO:0042296 IDA
    ubiquitin binding GO:0043130 EXP
    ubiquitin conjugating enzyme activity GO:0061631 IBA
Biological Process
    protein polyubiquitination GO:0000209 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ISG15-protein conjugation GO:0032020 IBA
    ISG15-protein conjugation GO:0032020 IDA
    protein modification process GO:0036211 TAS
    innate immune response GO:0045087 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IC
    cytosol GO:0005829 TAS
 Experiment description of studies that identified UBE2L6 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for UBE2L6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Reconstituted Complex Homo sapiens
2 SMC3 9126
Affinity Capture-MS Homo sapiens
3 ISG15 9636
Affinity Capture-MS Homo sapiens
4 GNPDA2 132789
Affinity Capture-MS Homo sapiens
5 XIAP  
Affinity Capture-Western Homo sapiens
6 RNF122  
Two-hybrid Homo sapiens
7 EEF1G 1937
Affinity Capture-MS Homo sapiens
8 SIAH2 6478
Affinity Capture-Western Homo sapiens
9 TARDBP 23435
Affinity Capture-MS Homo sapiens
10 RNF25 64320
Two-hybrid Homo sapiens
11 UBA1 7317
Biochemical Activity Homo sapiens
12 FLT3  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
14 TRIM62  
Two-hybrid Homo sapiens
15 MID1 4281
Two-hybrid Homo sapiens
16 CFL1 1072
Affinity Capture-MS Homo sapiens
17 RNF216  
Affinity Capture-Western Homo sapiens
18 DCTN3 11258
Affinity Capture-MS Homo sapiens
19 ARHGDIA 396
Two-hybrid Homo sapiens
20 SEPT9 10801
Affinity Capture-MS Homo sapiens
21 RUVBL1 8607
Affinity Capture-MS Homo sapiens
22 TRIM28 10155
Reconstituted Complex Homo sapiens
23 DTX3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
24 TIMM8A 1678
Affinity Capture-Western Homo sapiens
25 MCM2 4171
Affinity Capture-MS Homo sapiens
26 HERC5 51191
Reconstituted Complex Homo sapiens
27 HDAC2 3066
Affinity Capture-MS Homo sapiens
28 IQGAP1 8826
Affinity Capture-MS Homo sapiens
29 ACTR1A 10121
Affinity Capture-MS Homo sapiens
30 YWHAG 7532
Affinity Capture-MS Homo sapiens
31 RPS9 6203
Affinity Capture-MS Homo sapiens
32 ARIH2 10425
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
33 park  
Affinity Capture-Western Drosophila melanogaster
34 HSPA8 3312
Affinity Capture-MS Homo sapiens
35 TRIM8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
36 RUNX1  
Reconstituted Complex Homo sapiens
37 BAG5 9529
Affinity Capture-MS Homo sapiens
38 LONRF1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 RNF144A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
40 RNF4 6047
Two-hybrid Homo sapiens
41 RBX1 9978
Reconstituted Complex Homo sapiens
42 PARK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
43 UBAC2 337867
Affinity Capture-MS Homo sapiens
44 ABCF2 10061
Affinity Capture-MS Homo sapiens
45 PABPC1 26986
Affinity Capture-MS Homo sapiens
46 HERC6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PPA1 5464
Affinity Capture-MS Homo sapiens
48 TYK2 7297
Affinity Capture-Western Homo sapiens
49 DCP1A 55802
Affinity Capture-MS Homo sapiens
50 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
51 YWHAQ 10971
Affinity Capture-MS Homo sapiens
52 KPNB1 3837
Affinity Capture-MS Homo sapiens
53 DNM2 1785
Affinity Capture-MS Homo sapiens
54 RNF125  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
55 ITCH 83737
Affinity Capture-MS Homo sapiens
56 RPS15A 6210
Affinity Capture-MS Homo sapiens
57 RAD21 5885
Affinity Capture-Western Homo sapiens
58 OXA1L  
Affinity Capture-MS Homo sapiens
59 BIRC2  
Affinity Capture-Western Homo sapiens
60 GPIHBP1  
Affinity Capture-MS Homo sapiens
61 RNF40 9810
Affinity Capture-Western Homo sapiens
62 RNF144B  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
63 HADHB 3032
Affinity Capture-MS Homo sapiens
64 UBE3A 7337
FRET Homo sapiens
Biochemical Activity Homo sapiens
65 BAG2 9532
Affinity Capture-MS Homo sapiens
66 TCF7L2  
Affinity Capture-MS Homo sapiens
67 CTNNB1 1499
Affinity Capture-MS Homo sapiens
68 UBE2L6 9246
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
69 RNF150  
Two-hybrid Homo sapiens
70 RNF19A  
Affinity Capture-Western Homo sapiens
71 YWHAZ 7534
Affinity Capture-MS Homo sapiens
72 RPL3 6122
Affinity Capture-MS Homo sapiens
73 MAP3K1 4214
Two-hybrid Homo sapiens
74 PCBP1 5093
Affinity Capture-MS Homo sapiens
75 SPEN 23013
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
76 TRIM27  
Two-hybrid Homo sapiens
77 RPL12 6136
Affinity Capture-MS Homo sapiens
78 LMNA 4000
Affinity Capture-MS Homo sapiens
79 HUWE1 10075
Affinity Capture-MS Homo sapiens
80 PHGDH 26227
Affinity Capture-MS Homo sapiens
81 CBLC  
Two-hybrid Homo sapiens
82 IMMT 10989
Affinity Capture-MS Homo sapiens
83 TRAF7  
Two-hybrid Homo sapiens
84 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
85 MUL1  
Two-hybrid Homo sapiens
86 TRIM13  
Reconstituted Complex Homo sapiens
87 Rbck1  
Two-hybrid Mus musculus
88 Arih2  
Two-hybrid Mus musculus
89 RNF19B  
Affinity Capture-Western Homo sapiens
90 RUVBL2 10856
Affinity Capture-MS Homo sapiens
91 DNAJA2 10294
Affinity Capture-MS Homo sapiens
92 UBA7 7318
Biochemical Activity Homo sapiens
93 MEN1 4221
Affinity Capture-MS Homo sapiens
94 PTBP1 5725
Affinity Capture-MS Homo sapiens
95 DCTN2 10540
Affinity Capture-MS Homo sapiens
96 PJA2  
Two-hybrid Homo sapiens
97 TRIM25 7706
Affinity Capture-Western Homo sapiens
98 TRIP12 9320
Affinity Capture-MS Homo sapiens
99 WWP2 11060
Affinity Capture-MS Homo sapiens
100 CDK9 1025
Affinity Capture-MS Homo sapiens
101 ATP1A1 476
Affinity Capture-MS Homo sapiens
102 RPL27 6155
Affinity Capture-MS Homo sapiens
103 TOPORS  
Two-hybrid Homo sapiens
104 KRAS 3845
Synthetic Lethality Homo sapiens
105 GNB2L1 10399
Affinity Capture-MS Homo sapiens
106 WFS1 7466
Affinity Capture-MS Homo sapiens
107 UBA6 55236
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
108 RBCK1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
109 RANBP2 5903
Affinity Capture-MS Homo sapiens
110 ARIH1 25820
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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