Gene description for SIAH2
Gene name siah E3 ubiquitin protein ligase 2
Gene symbol SIAH2
Other names/aliases hSiah2
Species Homo sapiens
 Database cross references - SIAH2
ExoCarta ExoCarta_6478
Entrez Gene 6478
HGNC 10858
MIM 602213
UniProt O43255  
 SIAH2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for SIAH2
Molecular Function
    ubiquitin conjugating enzyme binding GO:0031624 IEA
    protein binding GO:0005515 IPI
    transcription corepressor activity GO:0003714 TAS
    ligase activity GO:0016874 IEA
    ubiquitin-protein transferase activity GO:0004842 IEA
    zinc ion binding GO:0008270 IEA
Biological Process
    protein ubiquitination GO:0016567 IEA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    axon guidance GO:0007411 TAS
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IMP
    small GTPase mediated signal transduction GO:0007264 TAS
    negative regulation of canonical Wnt signaling pathway GO:0090090 IGI
    cellular protein catabolic process GO:0044257 IGI
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    regulation of protein ubiquitination GO:0031396 IEA
    negative regulation of nucleic acid-templated transcription GO:1903507 TAS
    cell cycle GO:0007049 IEA
Subcellular Localization
    neuron projection GO:0043005 IEA
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    early endosome GO:0005769 IEA
    cytoplasm GO:0005737 TAS
    neuronal cell body GO:0043025 IEA
 Experiment description of studies that identified SIAH2 in exosomes
Experiment ID 237
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for SIAH2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DCC  
Invitro Homo sapiens
2 PEG3 5178
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
3 POU2AF1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 SYP  
Invivo Homo sapiens
Invitro Homo sapiens
5 VAV1 7409
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
6 TRAF2  
Reconstituted Complex Homo sapiens
7 PEG10 23089
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
8 UBE2L6  
Invivo Homo sapiens
9 RBBP8  
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which SIAH2 is involved
Netrin-1 signaling TAS Reactome

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