Gene description for VAV1
Gene name vav 1 guanine nucleotide exchange factor
Gene symbol VAV1
Other names/aliases VAV
Species Homo sapiens
 Database cross references - VAV1
ExoCarta ExoCarta_7409
Entrez Gene 7409
HGNC 12657
MIM 164875
UniProt P15498  
 VAV1 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for VAV1
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    sequence-specific DNA binding transcription factor activity GO:0003700 TAS
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    Rac guanyl-nucleotide exchange factor activity GO:0030676 IEA
Biological Process
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    reactive oxygen species metabolic process GO:0072593 IEA
    G-protein coupled receptor signaling pathway GO:0007186 IEA
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    platelet activation GO:0030168 TAS
    integrin-mediated signaling pathway GO:0007229 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    innate immune response GO:0045087 TAS
    blood coagulation GO:0007596 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    positive regulation of GTPase activity GO:0043547 TAS
    apoptotic signaling pathway GO:0097190 TAS
    T cell activation GO:0042110 IEA
    neutrophil chemotaxis GO:0030593 IEA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    regulation of transcription, DNA-templated GO:0006355 TAS
    T cell costimulation GO:0031295 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    cell-cell junction GO:0005911 IEA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified VAV1 in exosomes
1
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VAV1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BLNK  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
2 BTK 695
Affinity Capture-Western Homo sapiens
3 LAT 27040
Reconstituted Complex Homo sapiens
4 EMD  
Two-hybrid Homo sapiens
5 PTPRC 5788
Invivo Homo sapiens
Invitro Homo sapiens
6 PAG1  
Reconstituted Complex Homo sapiens
7 CD19 930
Invivo Homo sapiens
8 SIAH2 6478
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
9 SLA  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 ARHGDIB 397
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
11 PTPN6 5777
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
12 MAPK1 5594
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
13 ZYX 7791
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
14 ABL1 25
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 PTK2B 2185
Invivo Homo sapiens
Invitro Homo sapiens
16 JAK2 3717
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
17 SOCS1  
Affinity Capture-Western Homo sapiens
18 INSR 3643
Invivo Homo sapiens
Invitro Homo sapiens
19 EGFR 1956
Affinity Capture-Western Homo sapiens
20 FCER1A  
Affinity Capture-Western Homo sapiens
21 CBLB  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
22 PRKCQ 5588
Affinity Capture-Western Homo sapiens
Phenotypic Enhancement Homo sapiens
Affinity Capture-Western Homo sapiens
23 MAP2K1 5604
Affinity Capture-Western Homo sapiens
24 HNRNPK 3190
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
25 RACGAP1 29127
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
26 CDC42 998
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
27 TEC 7006
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
28 JUN 3725
Biochemical Activity Homo sapiens
29 IL6ST 3572
Invivo Homo sapiens
30 EZH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
31 FYN 2534
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
32 CBL 867
Invivo Homo sapiens
Invitro Homo sapiens
33 TYK2 7297
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
34 PDGFRB 5159
Affinity Capture-Western Homo sapiens
35 LCP2 3937
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
36 SHB  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
37 ZAP70 7535
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
38 XRCC6 2547
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
39 RAF1 5894
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
40 PRLR  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
41 DOCK2 1794
Invitro Homo sapiens
42 PIK3R1 5295
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
43 SYK 6850
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
44 RAC1 5879
Affinity Capture-Western Homo sapiens
45 SH3BP2  
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
46 S100B  
Invivo Homo sapiens
Invitro Homo sapiens
47 PLCG1 5335
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
48 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
49 XRCC5 7520
Affinity Capture-Western Homo sapiens
50 EPOR  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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