Gene description for BTK
Gene name Bruton agammaglobulinemia tyrosine kinase
Gene symbol BTK
Other names/aliases AGMX1
AT
ATK
BPK
IMD1
PSCTK1
XLA
Species Homo sapiens
 Database cross references - BTK
ExoCarta ExoCarta_695
Vesiclepedia VP_695
Entrez Gene 695
HGNC 1133
MIM 300300
UniProt Q06187  
 BTK identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for BTK
Molecular Function
    protein tyrosine kinase activity GO:0004713 EXP
    protein tyrosine kinase activity GO:0004713 IDA
    protein tyrosine kinase activity GO:0004713 TAS
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IBA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IDA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IGI
    non-membrane spanning protein tyrosine kinase activity GO:0004715 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 TAS
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    phospholipase activator activity GO:0016004 IDA
    histone H3Y41 kinase activity GO:0035401 IEA
    identical protein binding GO:0042802 IPI
    phospholipase binding GO:0043274 IPI
    metal ion binding GO:0046872 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    neutrophil homeostasis GO:0001780 IEA
    positive regulation of type III hypersensitivity GO:0001805 IEA
    positive regulation of type I hypersensitivity GO:0001812 IEA
    adaptive immune response GO:0002250 IBA
    adaptive immune response GO:0002250 TAS
    B cell affinity maturation GO:0002344 IEA
    histamine secretion by mast cell GO:0002553 IEA
    positive regulation of immunoglobulin production GO:0002639 IEA
    regulation of B cell cytokine production GO:0002721 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    regulation of B cell apoptotic process GO:0002902 TAS
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 TAS
    mesoderm development GO:0007498 TAS
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IGI
    calcium-mediated signaling GO:0019722 TAS
    proteoglycan catabolic process GO:0030167 IEA
    negative regulation of B cell proliferation GO:0030889 IEA
    positive regulation of B cell proliferation GO:0030890 IEA
    response to lipopolysaccharide GO:0032496 IEA
    negative regulation of interleukin-10 production GO:0032693 IEA
    positive regulation of interleukin-6 production GO:0032755 IEA
    positive regulation of tumor necrosis factor production GO:0032760 IEA
    cellular response to reactive oxygen species GO:0034614 IEA
    intracellular signal transduction GO:0035556 IDA
    intracellular signal transduction GO:0035556 IGI
    intracellular signal transduction GO:0035556 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    B cell activation GO:0042113 TAS
    innate immune response GO:0045087 TAS
    positive regulation of B cell differentiation GO:0045579 TAS
    cell maturation GO:0048469 IEA
    positive regulation of phagocytosis GO:0050766 IEA
    T cell receptor signaling pathway GO:0050852 IBA
    B cell receptor signaling pathway GO:0050853 IBA
    B cell receptor signaling pathway GO:0050853 IDA
    B cell receptor signaling pathway GO:0050853 TAS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
    monocyte proliferation GO:0061516 IEA
    cellular response to molecule of fungal origin GO:0071226 IEA
    apoptotic signaling pathway GO:0097190 TAS
    cellular response to interleukin-7 GO:0098761 IEA
    positive regulation of cGAS/STING signaling pathway GO:0141111 IDA
    positive regulation of interleukin-17A production GO:0150153 IEA
    positive regulation of NLRP3 inflammasome complex assembly GO:1900227 IDA
    positive regulation of synoviocyte proliferation GO:1901647 IEA
    eosinophil homeostasis GO:1990959 IEA
Subcellular Localization
    nucleus GO:0005634 TAS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cytoplasmic vesicle GO:0031410 IEA
    membrane raft GO:0045121 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified BTK in exosomes
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for BTK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AFG3L2 10939
Affinity Capture-MS Homo sapiens
2 NDUFS1 4719
Affinity Capture-MS Homo sapiens
3 STAT5A 6776
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
4 MYLK2 85366
Affinity Capture-MS Homo sapiens
5 DBN1 1627
Affinity Capture-MS Homo sapiens
6 IRAK1  
Affinity Capture-Western Homo sapiens
7 GNAQ 2776
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
9 RPS6KB2  
Biochemical Activity Homo sapiens
10 APP 351
Reconstituted Complex Homo sapiens
11 TLR8  
Two-hybrid Homo sapiens
12 RPS3 6188
Affinity Capture-MS Homo sapiens
13 EPHA8  
Affinity Capture-MS Homo sapiens
14 WAS 7454
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 SH3BP5 9467
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 PLCG2 5336
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 PLAU 5328
Two-hybrid Homo sapiens
18 Nedd4 25489
Biochemical Activity Rattus norvegicus
19 PRC1 9055
Affinity Capture-MS Homo sapiens
20 TLR6  
Two-hybrid Homo sapiens
21 KHDRBS1 10657
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
22 SYK 6850
Affinity Capture-Western Homo sapiens
23 EWSR1 2130
Affinity Capture-Western Homo sapiens
24 KRT5 3852
Affinity Capture-MS Homo sapiens
25 KATNB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 Mbp 17196
Protein-peptide Mus musculus
27 CBLB 868
Affinity Capture-Western Homo sapiens
28 POTEKP 440915
Affinity Capture-MS Homo sapiens
29 TWF1 5756
Affinity Capture-MS Homo sapiens
30 KIF14 9928
Affinity Capture-MS Homo sapiens
31 GTF2I 2969
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
32 AURKA 6790
Reconstituted Complex Homo sapiens
33 ATP5A1 498
Affinity Capture-MS Homo sapiens
34 FASLG 356
Protein-peptide Homo sapiens
35 VAV1 7409
Affinity Capture-Western Homo sapiens
36 COASY 80347
Biochemical Activity Homo sapiens
37 SPTA1  
Affinity Capture-MS Homo sapiens
38 BCL2L11  
Reconstituted Complex Homo sapiens
39 HCK 3055
Reconstituted Complex Homo sapiens
40 ERBB3 2065
Reconstituted Complex Homo sapiens
41 LYN 4067
Reconstituted Complex Homo sapiens
42 CBL 867
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
43 RPS6KB1 6198
Biochemical Activity Homo sapiens
44 FYN 2534
Reconstituted Complex Homo sapiens
45 MEOX2  
Two-hybrid Homo sapiens
46 TTC9C 283237
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 BTK 695
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
48 MPP7 143098
Co-fractionation Homo sapiens
49 ATP5B 506
Affinity Capture-MS Homo sapiens
50 TIRAP  
Affinity Capture-Western Homo sapiens
51 ARHGAP17 55114
Reconstituted Complex Homo sapiens
52 ACTN1 87
Affinity Capture-MS Homo sapiens
53 SFPQ 6421
Affinity Capture-MS Homo sapiens
54 HADHA 3030
Affinity Capture-MS Homo sapiens
55 HRNR 388697
Affinity Capture-MS Homo sapiens
56 TUBB4B 10383
Affinity Capture-MS Homo sapiens
57 PRKD1 5587
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
58 HSP90AB3P 3327
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 HSP90AA5P 730211
Affinity Capture-MS Homo sapiens
60 MYC  
Dosage Lethality Homo sapiens
61 CORO1C 23603
Affinity Capture-MS Homo sapiens
62 GAB1  
Reconstituted Complex Homo sapiens
63 TUBA4A 7277
Affinity Capture-MS Homo sapiens
64 ARID3A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
65 JAK1 3716
Affinity Capture-Western Homo sapiens
66 MAP3K4 4216
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 EEF1A2 1917
Affinity Capture-MS Homo sapiens
68 MET 4233
Reconstituted Complex Homo sapiens
69 TLR4  
Two-hybrid Homo sapiens
70 MDM2  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
71 FADD 8772
Negative Genetic Homo sapiens
72 GRB2 2885
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
73 TP53 7157
Affinity Capture-MS Homo sapiens
74 DDX3X 1654
Affinity Capture-MS Homo sapiens
75 CDC37 11140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 SMURF1 57154
Biochemical Activity Homo sapiens
77 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 GNB1 2782
Affinity Capture-Western Homo sapiens
79 DDX58 23586
Affinity Capture-RNA Homo sapiens
80 LPXN 9404
Affinity Capture-MS Homo sapiens
81 TLR9  
Two-hybrid Homo sapiens
82 ACTA1 58
Affinity Capture-MS Homo sapiens
83 GAPDH 2597
Affinity Capture-MS Homo sapiens
84 ARRB1 408
Two-hybrid Homo sapiens
85 IBTK 25998
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
86 FLNA 2316
Affinity Capture-MS Homo sapiens
87 MYD88 4615
Affinity Capture-Western Homo sapiens
88 STK11 6794
Negative Genetic Homo sapiens
89 AR 367
Reconstituted Complex Homo sapiens
90 BLNK  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
91 PRKCB 5579
Reconstituted Complex Homo sapiens
92 ANKRD54  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 CAV1 857
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
94 MED28  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which BTK is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Adaptive Immune System IEA Reactome
Antigen activates B Cell Receptor (BCR) leading to generation of second messengers TAS Reactome
Antigen activates B Cell Receptor (BCR) leading to generation of second messengers IEA Reactome
Antigen processing-Cross presentation TAS Reactome
Class I MHC mediated antigen processing & presentation TAS Reactome
DAP12 interactions TAS Reactome
DAP12 signaling TAS Reactome
Disease TAS Reactome
Diseases associated with the TLR signaling cascade TAS Reactome
Diseases of Immune System TAS Reactome
ER-Phagosome pathway TAS Reactome
Fc epsilon receptor (FCERI) signaling TAS Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated Ca+2 mobilization TAS Reactome
FCERI mediated Ca+2 mobilization IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis TAS Reactome
FCGR3A-mediated phagocytosis TAS Reactome
G alpha (12/13) signalling events TAS Reactome
G alpha (q) signalling events TAS Reactome
G beta:gamma signalling through BTK TAS Reactome
G-protein beta:gamma signalling TAS Reactome
GPCR downstream signalling TAS Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Infectious disease TAS Reactome
Innate Immune System TAS Reactome
Innate Immune System IEA Reactome
IRAK4 deficiency (TLR2/4) TAS Reactome
Leishmania infection TAS Reactome
Leishmania phagocytosis TAS Reactome
MyD88 deficiency (TLR2/4) TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Parasite infection TAS Reactome
Parasitic Infection Pathways TAS Reactome
Potential therapeutics for SARS TAS Reactome
Regulation of actin dynamics for phagocytic cup formation TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate WASPs and WAVEs TAS Reactome
SARS-CoV Infections TAS Reactome
Signal Transduction TAS Reactome
Signaling by GPCR TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by the B Cell Receptor (BCR) TAS Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
Viral Infection Pathways TAS Reactome





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