Gene description for BTK
Gene name Bruton agammaglobulinemia tyrosine kinase
Gene symbol BTK
Other names/aliases AGMX1
AT
ATK
BPK
IMD1
PSCTK1
XLA
Species Homo sapiens
 Database cross references - BTK
ExoCarta ExoCarta_695
Vesiclepedia VP_695
Entrez Gene 695
HGNC 1133
MIM 300300
UniProt Q06187  
 BTK identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for BTK
Molecular Function
    receptor binding GO:0005102 IBA
    identical protein binding GO:0042802 IPI
    ATP binding GO:0005524 TAS
    metal ion binding GO:0046872 IEA
    protein tyrosine kinase activity GO:0004713 TAS
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    protein binding GO:0005515 IPI
    non-membrane spanning protein tyrosine kinase activity GO:0004715 TAS
Biological Process
    B cell activation GO:0042113 TAS
    peptidyl-tyrosine autophosphorylation GO:0038083 IBA
    mesoderm development GO:0007498 TAS
    adaptive immune response GO:0002250 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    cell maturation GO:0048469 IEA
    regulation of cell proliferation GO:0042127 IBA
    positive regulation of B cell differentiation GO:0045579 TAS
    cellular response to molecule of fungal origin GO:0071226 IEA
    T cell receptor signaling pathway GO:0050852 IBA
    toll-like receptor signaling pathway GO:0002224 TAS
    innate immune response GO:0045087 TAS
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    B cell receptor signaling pathway GO:0050853 TAS
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    regulation of B cell apoptotic process GO:0002902 TAS
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    calcium-mediated signaling GO:0019722 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    cellular response to reactive oxygen species GO:0034614 IEA
    cell differentiation GO:0030154 IBA
    apoptotic signaling pathway GO:0097190 TAS
    intracellular signal transduction GO:0035556 TAS
    protein phosphorylation GO:0006468 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    histamine secretion by mast cell GO:0002553 IEA
    transcription, DNA-templated GO:0006351 IEA
    I-kappaB kinase/NF-kappaB signaling GO:0007249 IEA
    regulation of B cell cytokine production GO:0002721 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IEA
    membrane raft GO:0045121 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 TAS
    nucleus GO:0005634 TAS
    cytoplasmic vesicle GO:0031410 IEA
    plasma membrane GO:0005886 TAS
    mast cell granule GO:0042629 IEA
 Experiment description of studies that identified BTK in exosomes
1
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for BTK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BTK 695
Invitro Homo sapiens
Invitro Homo sapiens
2 ARHGAP17 55114
Reconstituted Complex Homo sapiens
3 CBLB 868
Affinity Capture-Western Homo sapiens
4 GNA12 2768
Invitro Homo sapiens
Invivo Homo sapiens
5 FAS 355
Invivo Homo sapiens
6 WASF2 10163
Invitro Homo sapiens
7 GTF2I 2969
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
8 VAV1 7409
Affinity Capture-Western Homo sapiens
9 GNAQ 2776
Invivo Homo sapiens
Invitro Homo sapiens
10 PRKD1 5587
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 HCK 3055
Reconstituted Complex Homo sapiens
12 RPS6KB2  
Biochemical Activity Homo sapiens
13 LYN 4067
Reconstituted Complex Homo sapiens
14 CBL 867
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
15 WAS 7454
Reconstituted Complex Homo sapiens
16 RPS6KB1 6198
Biochemical Activity Homo sapiens
17 SH3BP5 9467
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
18 PLCG2 5336
Affinity Capture-Western Homo sapiens
19 ARID3A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 JAK1 3716
Affinity Capture-Western Homo sapiens
21 IBTK 25998
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 FYN 2534
Reconstituted Complex Homo sapiens
23 KHDRBS1 10657
Affinity Capture-Western Homo sapiens
24 BLNK  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
25 PRKCB 5579
Reconstituted Complex Homo sapiens
26 EWSR1 2130
Affinity Capture-Western Homo sapiens
27 CAV1 857
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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