Experiment description of studies that identified GTF2I in exosomes

1 Experiment ID 234 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH EV Cytosolic markers ✘ EV Membrane markers ✔ HSP90B1 EV Negative markers ✔ qNano EV Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-C3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 237 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH EV Cytosolic markers ✘ EV Membrane markers ✔ HSP90B1 EV Negative markers ✔ qNano EV Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

3 Experiment ID 211 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ cytochrome c|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 212 ISEV standards ✔ CEM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ EpCAM|TFRC EV Membrane markers ✔ Cytochrome C|GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 217 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ CD81|CD9|CD63 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for GTF2I

Protein Interactor ExoCarta ID Identification method PubMed Species 1 STAT1 6772 Invivo Homo sapiens Invitro Homo sapiens 2 SRF   Invivo Homo sapiens Invivo Homo sapiens Invitro Homo sapiens Invitro Homo sapiens 3 YY1 7528 Invivo Homo sapiens Invitro Homo sapiens 4 LSM8   Two-hybrid Homo sapiens 5 STAT3 6774 Invivo Homo sapiens Invitro Homo sapiens 6 USF1   Invivo Homo sapiens Invitro Homo sapiens Reconstituted Complex Homo sapiens 7 BTK 695 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 8 ZBTB17   Invivo Homo sapiens Invitro Homo sapiens Two-hybrid Homo sapiens 9 MAPK3 5595 Invivo Homo sapiens Invitro Homo sapiens 10 HDAC3 8841 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 11 KDM1A 23028 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 12 HDAC2 3066 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 13 PRRX1   Invitro Homo sapiens Invivo Homo sapiens 14 PTP4A3   Affinity Capture-MS Homo sapiens 15 HDAC1 3065 Affinity Capture-Western Homo sapiens 16 PRKG1   Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 17 ATF6   Invitro Homo sapiens Invivo Homo sapiens 18 PIAS2   Invivo Homo sapiens Invitro Homo sapiens Two-hybrid Homo sapiens 19 MYC   Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 20 PHF21A   Affinity Capture-Western Homo sapiens View the network

Pathways in which GTF2I is involved

No pathways found