Gene description for GTF2I
Gene name general transcription factor IIi
Gene symbol GTF2I
Other names/aliases BAP135
BTKAP1
DIWS
GTFII-I
IB291
SPIN
TFII-I
WBS
WBSCR6
Species Homo sapiens
 Database cross references - GTF2I
ExoCarta ExoCarta_2969
Entrez Gene 2969
HGNC 4659
MIM 601679
UniProt P78347  
 GTF2I identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for GTF2I
Molecular Function
    sequence-specific DNA binding transcription factor activity GO:0003700 NAS
    protein binding GO:0005515 IPI
    DNA binding GO:0003677 IEA
    mitogen-activated protein kinase binding GO:0051019 IEA
Biological Process
    signal transduction GO:0007165 TAS
    negative regulation of angiogenesis GO:0016525 IDA
    negative regulation of cytosolic calcium ion concentration GO:0051481 IEA
    transcription from RNA polymerase II promoter GO:0006366 TAS
    regulation of transcription, DNA-templated GO:0006355 IEA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    transition between slow and fast fiber GO:0014886 IEA
Subcellular Localization
    neuronal cell body GO:0043025 IEA
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IEA
    nucleus GO:0005634 IDA
    cell projection GO:0042995 IEA
    membrane GO:0016020 IDA
 Experiment description of studies that identified GTF2I in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GTF2I
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STAT1 6772
Invivo Homo sapiens
Invitro Homo sapiens
2 SRF  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
3 YY1 7528
Invivo Homo sapiens
Invitro Homo sapiens
4 LSM8  
Two-hybrid Homo sapiens
5 STAT3 6774
Invivo Homo sapiens
Invitro Homo sapiens
6 USF1  
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
7 BTK 695
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 ZBTB17  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
9 MAPK3 5595
Invivo Homo sapiens
Invitro Homo sapiens
10 HDAC3 8841
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 KDM1A 23028
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 HDAC2 3066
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 PRRX1  
Invitro Homo sapiens
Invivo Homo sapiens
14 PTP4A3  
Affinity Capture-MS Homo sapiens
15 HDAC1 3065
Affinity Capture-Western Homo sapiens
16 PRKG1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 ATF6  
Invitro Homo sapiens
Invivo Homo sapiens
18 PIAS2  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
19 MYC  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 PHF21A  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which GTF2I is involved
No pathways found





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