Gene description for MAP3K3
Gene name mitogen-activated protein kinase kinase kinase 3
Gene symbol MAP3K3
Other names/aliases MAPKKK3
MEKK3
Species Homo sapiens
 Database cross references - MAP3K3
ExoCarta ExoCarta_4215
Vesiclepedia VP_4215
Entrez Gene 4215
HGNC 6855
MIM 602539
UniProt Q99759  
 MAP3K3 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MAP3K3
Molecular Function
    protein kinase activity GO:0004672 IDA
    ATP binding GO:0005524 IEA
    MAP kinase kinase activity GO:0004708 IBA
    protein binding GO:0005515 IPI
    MAP kinase kinase kinase activity GO:0004709 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    positive regulation of p38MAPK cascade GO:1900745 IEA
    MAPK cascade GO:0000165 TAS
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IEP
    activation of MAPK activity GO:0000187 IBA
    intracellular signal transduction GO:0035556 IDA
    blood vessel development GO:0001568 IEA
    protein autophosphorylation GO:0046777 IDA
    activation of MAPKK activity GO:0000186 TAS
Subcellular Localization
    intracellular GO:0005622 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MAP3K3 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAP3K3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSPA4 3308
Affinity Capture-MS Homo sapiens
2 GAB1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 YWHAE 7531
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
4 TRAF7  
Affinity Capture-MS Homo sapiens
5 CDC37 11140
Affinity Capture-MS Homo sapiens
6 BRCA1 672
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
7 YWHAG 7532
Affinity Capture-MS Homo sapiens
8 MARK2 2011
Affinity Capture-MS Homo sapiens
9 ZBTB16  
Two-hybrid Homo sapiens
10 HSP90AA2P 3324
Affinity Capture-MS Homo sapiens
11 MAP2K5  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 PFDN2 5202
Affinity Capture-MS Homo sapiens
13 PDRG1  
Affinity Capture-MS Homo sapiens
14 RIPK1 8737
Invivo Homo sapiens
15 SPAG9 9043
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MAP3K3 is involved
PathwayEvidenceSource
Interleukin-1 signaling TAS Reactome





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