Gene description for MAP3K3
Gene name mitogen-activated protein kinase kinase kinase 3
Gene symbol MAP3K3
Other names/aliases MAPKKK3
MEKK3
Species Homo sapiens
 Database cross references - MAP3K3
ExoCarta ExoCarta_4215
Vesiclepedia VP_4215
Entrez Gene 4215
HGNC 6855
MIM 602539
UniProt Q99759  
 MAP3K3 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MAP3K3
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    JUN kinase kinase kinase activity GO:0004706 IEA
    MAP kinase kinase kinase activity GO:0004709 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    MAPK cascade GO:0000165 TAS
    blood vessel development GO:0001568 IEA
    intracellular signal transduction GO:0035556 IDA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IEP
    protein autophosphorylation GO:0046777 IDA
    positive regulation of p38MAPK cascade GO:1900745 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MAP3K3 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAP3K3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 TRAF7  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
4 BIRC2  
Biochemical Activity Homo sapiens
5 CHUK 1147
Co-localization Homo sapiens
6 Anapc5 59008
Affinity Capture-MS Mus musculus
7 MAP2K7 5609
Biochemical Activity Homo sapiens
8 XIAP  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
9 CCM2 83605
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 NEDD4 4734
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
11 FLNA 2316
Co-localization Homo sapiens
12 MAP2K4 6416
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
13 XPO1 7514
Affinity Capture-MS Homo sapiens
14 MBP 4155
Biochemical Activity Homo sapiens
15 KIFC3 3801
Affinity Capture-MS Homo sapiens
16 GTF2I 2969
Two-hybrid Homo sapiens
17 YWHAE 7531
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
18 CDC37 11140
Affinity Capture-MS Homo sapiens
19 BRCA1 672
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
20 TRAF6 7189
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
21 STRN3 29966
Affinity Capture-Western Homo sapiens
22 SMURF1 57154
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
23 SQSTM1 8878
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
24 MAP3K2 10746
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
25 MAP2K5  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
26 YWHAB 7529
Affinity Capture-MS Homo sapiens
27 ZBTB16  
Two-hybrid Homo sapiens
28 FUS 2521
Affinity Capture-MS Homo sapiens
29 HSP90AA2P 3324
Affinity Capture-MS Homo sapiens
30 MAP3K7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
31 LATS1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
32 GAB1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
33 YWHAQ 10971
Affinity Capture-MS Homo sapiens
34 COPS5 10987
Two-hybrid Homo sapiens
35 PDCD10 11235
Affinity Capture-Western Homo sapiens
36 PRKACA 5566
Co-localization Homo sapiens
37 MAP2K3 5606
Biochemical Activity Homo sapiens
38 MARK2 2011
Affinity Capture-MS Homo sapiens
39 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 YAP1 10413
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
41 PFDN2 5202
Affinity Capture-MS Homo sapiens
42 Nedd4 25489
Biochemical Activity Rattus norvegicus
43 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
44 MTOR 2475
Affinity Capture-Western Homo sapiens
45 IP6K3  
Affinity Capture-MS Homo sapiens
46 SPAG9 9043
Affinity Capture-Western Homo sapiens
47 PPP2CA 5515
Affinity Capture-Western Homo sapiens
48 HSPA4 3308
Affinity Capture-MS Homo sapiens
49 RIPK1 8737
Affinity Capture-Western Homo sapiens
50 GLRX3 10539
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
51 CUL7 9820
Affinity Capture-MS Homo sapiens
52 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 NBR1 4077
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
54 MAP3K3 4215
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
55 WWTR1 25937
Affinity Capture-Western Homo sapiens
56 LATS2 26524
Affinity Capture-Western Homo sapiens
57 VWF 7450
Two-hybrid Homo sapiens
58 TOM1L1 10040
Two-hybrid Homo sapiens
59 CBY1  
Affinity Capture-MS Homo sapiens
60 PDRG1  
Affinity Capture-MS Homo sapiens
61 NEDD4L 23327
Biochemical Activity Homo sapiens
62 Cep85  
Affinity Capture-MS Mus musculus
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