Gene description for MTOR
Gene name mechanistic target of rapamycin (serine/threonine kinase)
Gene symbol MTOR
Other names/aliases FRAP
FRAP1
FRAP2
RAFT1
RAPT1
Species Homo sapiens
 Database cross references - MTOR
ExoCarta ExoCarta_2475
Entrez Gene 2475
HGNC 3942
MIM 601231
UniProt P42345  
 MTOR identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for MTOR
Molecular Function
    protein serine/threonine kinase activity GO:0004674 TAS
    protein binding GO:0005515 IPI
    TFIIIC-class transcription factor binding GO:0001156 IDA
    ATP binding GO:0005524 IEA
    RNA polymerase III type 1 promoter DNA binding GO:0001030 IDA
    drug binding GO:0008144 IEA
    RNA polymerase III type 3 promoter DNA binding GO:0001032 IDA
    RNA polymerase III type 2 promoter DNA binding GO:0001031 IDA
    kinase activity GO:0016301 TAS
    ribosome binding GO:0043022 IEA
    protein dimerization activity GO:0046983 IBA
    phosphoprotein binding GO:0051219 IPI
    protein domain specific binding GO:0019904 IEA
Biological Process
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of lipid biosynthetic process GO:0046889 IMP
    regulation of fatty acid beta-oxidation GO:0031998 IEA
    cellular response to hypoxia GO:0071456 ISS
    protein autophosphorylation GO:0046777 IDA
    response to amino acid GO:0043200 IDA
    regulation of myelination GO:0031641 IEA
    cellular response to heat GO:0034605 TAS
    cell cycle arrest GO:0007050 TAS
    regulation of actin cytoskeleton organization GO:0032956 IMP
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    phosphorylation GO:0016310 IDA
    double-strand break repair via homologous recombination GO:0000724 IBA
    negative regulation of macroautophagy GO:0016242 IEA
    protein catabolic process GO:0030163 TAS
    regulation of osteoclast differentiation GO:0045670 ISS
    negative regulation of NFAT protein import into nucleus GO:0051534 IEA
    negative regulation of mitochondrion degradation GO:1903147 NAS
    regulation of glycogen biosynthetic process GO:0005979 IEA
    regulation of cellular response to heat GO:1900034 TAS
    cellular response to nutrient levels GO:0031669 ISS
    growth GO:0040007 NAS
    positive regulation of endothelial cell proliferation GO:0001938 IEA
    positive regulation of stress fiber assembly GO:0051496 IEA
    regulation of carbohydrate utilization GO:0043610 IEA
    protein phosphorylation GO:0006468 IMP
    insulin receptor signaling pathway GO:0008286 TAS
    positive regulation of translation GO:0045727 IDA
    positive regulation of protein phosphorylation GO:0001934 IDA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    positive regulation of protein kinase B signaling GO:0051897 IEA
    positive regulation of actin filament polymerization GO:0030838 IEA
    response to stress GO:0006950 IMP
    positive regulation of myotube differentiation GO:0010831 IEA
    peptidyl-serine phosphorylation GO:0018105 IMP
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    response to nutrient GO:0007584 NAS
    innate immune response GO:0045087 TAS
    germ cell development GO:0007281 IEA
    gene expression GO:0010467 TAS
    positive regulation of transcription from RNA polymerase III promoter GO:0045945 IMP
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    regulation of response to food GO:0032095 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    TOR signaling GO:0031929 IMP
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    peptidyl-threonine phosphorylation GO:0018107 IEA
    T cell costimulation GO:0031295 TAS
    positive regulation of lamellipodium assembly GO:0010592 IEA
    signal transduction GO:0007165 NAS
    negative regulation of cell size GO:0045792 IEA
    cell growth GO:0016049 TAS
    ruffle organization GO:0031529 IEA
    negative regulation of autophagy GO:0010507 ISS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    regulation of protein kinase activity GO:0045859 IEA
    phosphatidylinositol-mediated signaling GO:0048015 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    phosphatidylinositol 3-kinase complex GO:0005942 NAS
    Golgi membrane GO:0000139 IEA
    PML body GO:0016605 ISS
    lysosomal membrane GO:0005765 IDA
    TORC1 complex GO:0031931 IMP
    lysosome GO:0005764 IDA
    cytosol GO:0005829 TAS
    TORC2 complex GO:0031932 IDA
    dendrite GO:0030425 IEA
    neuronal cell body GO:0043025 IEA
    endomembrane system GO:0012505 IDA
    mitochondrial outer membrane GO:0005741 IEA
    endoplasmic reticulum membrane GO:0005789 IEA
    nucleoplasm GO:0005654 TAS
    membrane GO:0016020 IDA
 Experiment description of studies that identified MTOR in exosomes
1
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MTOR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PA2G4 5036
Reconstituted Complex Homo sapiens
2 TERT  
Affinity Capture-Western Homo sapiens
3 PDK1  
Phenotypic Enhancement Homo sapiens
4 GDI2P  
Affinity Capture-Western Homo sapiens
5 PRKAA1 5562
Protein-peptide Homo sapiens
6 EIF3F 8665
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
7 MAPKAP1 79109
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 Rps6kb1  
Affinity Capture-Western Mus musculus
9 PRKCD 5580
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 EIF3C 8663
Affinity Capture-Western Homo sapiens
11 PPP2R2A 5520
Reconstituted Complex Homo sapiens
12 PFC  
Reconstituted Complex Homo sapiens
13 ABL1 25
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 LRPAP1  
Affinity Capture-Western Homo sapiens
15 RPTOR 57521
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 RICTOR 253260
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 RPS6KB1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
18 FKBP1A 2280
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
19 RSN  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
20 RHEB 6009
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
21 STAT1 6772
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 Rheb  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
23 Stat3  
Affinity Capture-Western Rattus norvegicus
24 MTOR 2475
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 GPHN  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
26 RAP1A 5906
Affinity Capture-Western Homo sapiens
27 EIF3B 8662
Affinity Capture-Western Homo sapiens
28 STAT3 6774
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
29 MLST8 64223
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
30 AKT1 207
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
31 EIF4EBP1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
32 UBQLN1 29979
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
33 IRS1 3667
Affinity Capture-Western Homo sapiens
34 PLD2 5338
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here