Gene description for KLC4
Gene name kinesin light chain 4
Gene symbol KLC4
Other names/aliases KNSL8
bA387M24.3
Species Homo sapiens
 Database cross references - KLC4
ExoCarta ExoCarta_89953
Vesiclepedia VP_89953
Entrez Gene 89953
HGNC 21624
UniProt Q9NSK0  
 KLC4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Thymus 23844026    
 Gene ontology annotations for KLC4
Molecular Function
    protein binding GO:0005515 IPI
    kinesin binding GO:0019894 IBA
Biological Process
    microtubule-based movement GO:0007018 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    kinesin complex GO:0005871 IEA
    microtubule GO:0005874 IEA
 Experiment description of studies that identified KLC4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KLC4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nedd4 25489
Biochemical Activity Rattus norvegicus
2 HOOK1  
Proximity Label-MS Homo sapiens
3 NECAB2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 KLC2 64837
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 COIL  
Two-hybrid Homo sapiens
6 PKP2 5318
Two-hybrid Homo sapiens
7 NAA40  
Affinity Capture-MS Homo sapiens
8 YWHAE 7531
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
9 NUP62 23636
Affinity Capture-MS Homo sapiens
10 SYNE4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
11 KPNA2 3838
Two-hybrid Homo sapiens
12 CBY1  
Affinity Capture-MS Homo sapiens
13 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
14 APEX1 328
Affinity Capture-RNA Homo sapiens
15 DIAPH1 1729
Co-fractionation Homo sapiens
16 KATNAL1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 CEP57L1  
Two-hybrid Homo sapiens
18 COPB2 9276
Co-fractionation Homo sapiens
19 KIF5B 3799
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 MEF2A  
Affinity Capture-MS Homo sapiens
21 PRC1 9055
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 KRT31 3881
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
24 Klc2  
Affinity Capture-MS Mus musculus
25 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 DDX6 1656
Two-hybrid Homo sapiens
27 KPTN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 MYCN  
Affinity Capture-MS Homo sapiens
29 Klc3  
Affinity Capture-MS Mus musculus
30 RBM14 10432
Two-hybrid Homo sapiens
31 LAMTOR1 55004
Proximity Label-MS Homo sapiens
32 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 C15orf59  
Affinity Capture-MS Homo sapiens
34 KRT8 3856
Proximity Label-MS Homo sapiens
35 KRT34  
Two-hybrid Homo sapiens
36 S100A2 6273
Affinity Capture-MS Homo sapiens
37 KRT39  
Affinity Capture-MS Homo sapiens
38 RUFY1 80230
Co-fractionation Homo sapiens
39 KLC1 3831
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 CLSTN1 22883
Affinity Capture-MS Homo sapiens
41 TCEA2  
Affinity Capture-MS Homo sapiens
42 PRPS2 5634
Affinity Capture-MS Homo sapiens
43 KIF5A 3798
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
44 SERTAD1  
Two-hybrid Homo sapiens
45 KRT16 3868
Two-hybrid Homo sapiens
46 KLC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 NTRK1 4914
Affinity Capture-MS Homo sapiens
48 MEOX2  
Two-hybrid Homo sapiens
49 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ENTHD1  
Affinity Capture-MS Homo sapiens
51 PLXNA3 55558
Affinity Capture-MS Homo sapiens
52 MYCL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 ABI3 51225
Two-hybrid Homo sapiens
54 CEP57  
Two-hybrid Homo sapiens
55 S100P 6286
Affinity Capture-MS Homo sapiens
56 KRT33B 3884
Two-hybrid Homo sapiens
57 AKAP9 10142
Two-hybrid Homo sapiens
58 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 HOOK3 84376
Proximity Label-MS Homo sapiens
60 NUP43 348995
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 OSBPL5 114879
Affinity Capture-MS Homo sapiens
62 AZIN1 51582
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 LRRC16A 55604
Co-fractionation Homo sapiens
64 CDR2  
Affinity Capture-MS Homo sapiens
65 KRT15 3866
Two-hybrid Homo sapiens
66 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 CHRNA9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 TADA2A  
Two-hybrid Homo sapiens
69 NUCB1 4924
Co-fractionation Homo sapiens
70 MTOR 2475
Affinity Capture-MS Homo sapiens
71 USHBP1  
Two-hybrid Homo sapiens
72 PCM1 5108
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
73 KRT19 3880
Proximity Label-MS Homo sapiens
74 MCM3AP  
Affinity Capture-MS Homo sapiens
75 KIF2B 84643
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 PARP16 54956
Affinity Capture-MS Homo sapiens
77 HVCN1 84329
Affinity Capture-MS Homo sapiens
78 TRIP6 7205
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 TRAK2 66008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
81 KIF14 9928
Affinity Capture-MS Homo sapiens
82 TRAK1 22906
Affinity Capture-MS Homo sapiens
83 SLC16A9  
Two-hybrid Homo sapiens
84 CASK 8573
Two-hybrid Homo sapiens
85 CLIP2 7461
Affinity Capture-MS Homo sapiens
86 Kif5b 16573
Affinity Capture-MS Mus musculus
87 PUF60 22827
Affinity Capture-MS Homo sapiens
88 EPB41L2 2037
Affinity Capture-MS Homo sapiens
89 KIF5C 3800
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
90 IARS2 55699
Affinity Capture-MS Homo sapiens
91 BRK1 55845
Affinity Capture-MS Homo sapiens
92 ANAPC2 29882
Proximity Label-MS Homo sapiens
93 NLN 57486
Affinity Capture-MS Homo sapiens
94 EZH2  
Affinity Capture-MS Homo sapiens
95 CDK9 1025
Affinity Capture-MS Homo sapiens
96 RAB11A 8766
Proximity Label-MS Homo sapiens
97 NDEL1 81565
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
98 UMPS 7372
Affinity Capture-MS Homo sapiens
99 PDE4DIP 9659
Two-hybrid Homo sapiens
100 KRT13 3860
Two-hybrid Homo sapiens
101 CUL4A 8451
Affinity Capture-MS Homo sapiens
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