Gene description for RUFY1
Gene name RUN and FYVE domain containing 1
Gene symbol RUFY1
Other names/aliases RABIP4
ZFYVE12
Species Homo sapiens
 Database cross references - RUFY1
ExoCarta ExoCarta_80230
Vesiclepedia VP_80230
Entrez Gene 80230
HGNC 19760
MIM 610327
UniProt Q96T51  
 RUFY1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RUFY1
Molecular Function
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IEA
    SH3 domain binding GO:0017124 IPI
    protein-macromolecule adaptor activity GO:0030674 IDA
    SH2 domain binding GO:0042169 IPI
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    endocytosis GO:0006897 IEA
    small GTPase-mediated signal transduction GO:0007264 IEA
    protein transport GO:0015031 IBA
    regulation of endocytosis GO:0030100 IBA
    regulation of endocytosis GO:0030100 IPI
    endosomal vesicle fusion GO:0034058 IDA
    early endosome to Golgi transport GO:0034498 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    endosome GO:0005768 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    nuclear speck GO:0016607 IDA
    early endosome membrane GO:0031901 IDA
    early endosome membrane GO:0031901 TAS
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified RUFY1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RUFY1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGOLN2 10618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 KRT37 8688
Affinity Capture-MS Homo sapiens
3 SYCE1  
Affinity Capture-MS Homo sapiens
4 DYNC1I2 1781
Proximity Label-MS Homo sapiens
5 GIT2 9815
Two-hybrid Homo sapiens
6 CCDC183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 LAMP2 3920
Proximity Label-MS Homo sapiens
8 DIEXF  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 PMF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 VPS26A 9559
Co-fractionation Homo sapiens
11 CAPZB 832
Co-fractionation Homo sapiens
12 SRA1 10011
Affinity Capture-MS Homo sapiens
13 TUBB 203068
Affinity Capture-MS Homo sapiens
14 COPB2 9276
Co-fractionation Homo sapiens
15 RAB3IP  
Two-hybrid Homo sapiens
16 TELO2 9894
Co-fractionation Homo sapiens
17 COL4A3BP 10087
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TIMM23 100287932
Affinity Capture-MS Homo sapiens
19 GRIK4  
Co-fractionation Homo sapiens
20 KLC4 89953
Co-fractionation Homo sapiens
21 COMT 1312
Affinity Capture-MS Homo sapiens
22 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 RAB39B 116442
Two-hybrid Homo sapiens
24 RAB35 11021
Proximity Label-MS Homo sapiens
25 PKN2 5586
Affinity Capture-MS Homo sapiens
26 LAMTOR1 55004
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ESR1  
Affinity Capture-MS Homo sapiens
28 FAS 355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PFN1 5216
Proximity Label-MS Homo sapiens
30 HSPBP1 23640
Affinity Capture-MS Homo sapiens
31 VSIG1  
Affinity Capture-MS Homo sapiens
32 AP3M1 26985
Reconstituted Complex Homo sapiens
33 CD70 970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RAB14 51552
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
35 RUFY1 80230
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
36 PARK2  
Affinity Capture-MS Homo sapiens
37 HCK 3055
Affinity Capture-MS Homo sapiens
38 TXNDC17 84817
Two-hybrid Homo sapiens
39 RABEP1 9135
Co-fractionation Homo sapiens
40 COPG1 22820
Co-fractionation Homo sapiens
41 FAM9C  
Affinity Capture-MS Homo sapiens
42 TUBA1A 7846
Affinity Capture-MS Homo sapiens
43 RAB4A 5867
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
44 NTRK1 4914
Affinity Capture-MS Homo sapiens
45 SYNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 PIAS2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
47 PEX14 5195
Proximity Label-MS Homo sapiens
48 PHACTR2  
Co-fractionation Homo sapiens
49 BMX  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
50 TTI1 9675
Co-fractionation Homo sapiens
51 RNF40 9810
Co-fractionation Homo sapiens
52 ST8SIA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 RAB5A 5868
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
54 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 GYPA  
Affinity Capture-MS Homo sapiens
56 CUL3 8452
Affinity Capture-MS Homo sapiens
57 KXD1 79036
Affinity Capture-MS Homo sapiens
58 FNTA 2339
Co-fractionation Homo sapiens
59 METTL21B  
Affinity Capture-MS Homo sapiens
60 FOSL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 SEC24A 10802
Co-fractionation Homo sapiens
62 SNX8 29886
Affinity Capture-MS Homo sapiens
63 RAB9A 9367
Proximity Label-MS Homo sapiens
64 GOLGA1  
Proximity Label-MS Homo sapiens
65 ACOX3 8310
Co-fractionation Homo sapiens
66 HSPA1A 3303
Affinity Capture-MS Homo sapiens
67 SART3 9733
Co-fractionation Homo sapiens
68 LRRC40 55631
Affinity Capture-MS Homo sapiens
69 GRK6 2870
Affinity Capture-MS Homo sapiens
70 AP3S1 1176
Reconstituted Complex Homo sapiens
71 RALBP1 10928
Affinity Capture-MS Homo sapiens
72 PRMT3 10196
Co-fractionation Homo sapiens
73 FASN 2194
Positive Genetic Homo sapiens
74 ANKFY1 51479
Proximity Label-MS Homo sapiens
75 COPZ1 22818
Co-fractionation Homo sapiens
76 CCT8 10694
Co-fractionation Homo sapiens
77 RUFY3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 WDFY2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 KIAA0226L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 GORASP1 64689
Proximity Label-MS Homo sapiens
81 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
82 NLN 57486
Affinity Capture-MS Homo sapiens
83 RUFY2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 ITSN2 50618
Two-hybrid Homo sapiens
85 RAB2A 5862
Proximity Label-MS Homo sapiens
86 LAGE3  
Affinity Capture-MS Homo sapiens
87 TRIM23 373
Two-hybrid Homo sapiens
88 RAB11A 8766
Proximity Label-MS Homo sapiens
89 AP3B2 8120
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
90 RAB5C 5878
Proximity Label-MS Homo sapiens
91 NFIC 4782
Co-fractionation Homo sapiens
92 AP3D1 8943
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
93 TRAV20  
Affinity Capture-MS Homo sapiens
94 CAV1 857
Proximity Label-MS Homo sapiens
95 ASPA  
Affinity Capture-MS Homo sapiens
96 PSME4 23198
Co-fractionation Homo sapiens
View the network image/svg+xml



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