Gene description for ITSN2
Gene name intersectin 2
Gene symbol ITSN2
Other names/aliases PRO2015
SH3D1B
SH3P18
SWA
SWAP
Species Homo sapiens
 Database cross references - ITSN2
ExoCarta ExoCarta_50618
Vesiclepedia VP_50618
Entrez Gene 50618
HGNC 6184
MIM 604464
UniProt Q9NZM3  
 ITSN2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for ITSN2
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IEA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    molecular adaptor activity GO:0060090 IBA
Biological Process
    endosomal transport GO:0016197 IBA
    cell differentiation GO:0030154 IEA
    clathrin-dependent synaptic vesicle endocytosis GO:0150007 IBA
    positive regulation of dendrite extension GO:1903861 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    presynaptic membrane GO:0042734 IBA
    extracellular exosome GO:0070062 HDA
    intracellular vesicle GO:0097708 IBA
 Experiment description of studies that identified ITSN2 in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
5
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ITSN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP2S1 1175
Affinity Capture-MS Homo sapiens
2 AP2B1 163
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 WIPF2 147179
Two-hybrid Homo sapiens
4 RBM22 55696
Affinity Capture-MS Homo sapiens
5 ATG9A 79065
Proximity Label-MS Homo sapiens
6 YTHDF1 54915
Two-hybrid Homo sapiens
7 KCTD10 83892
Two-hybrid Homo sapiens
8 SH3GL1 6455
Two-hybrid Homo sapiens
9 BTF3 689
Affinity Capture-MS Homo sapiens
10 PTN  
Two-hybrid Homo sapiens
11 FBXL19  
Affinity Capture-MS Homo sapiens
12 EPS15 2060
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
13 SNRNP40 9410
Affinity Capture-MS Homo sapiens
14 TAB1 10454
Affinity Capture-MS Homo sapiens
15 UBC 7316
Reconstituted Complex Homo sapiens
16 SRFBP1  
Affinity Capture-MS Homo sapiens
17 HOOK2  
Two-hybrid Homo sapiens
18 CLTB 1212
Proximity Label-MS Homo sapiens
19 MAP4K3 8491
Two-hybrid Homo sapiens
20 EPS8 2059
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
21 ANKRD17 26057
Two-hybrid Homo sapiens
22 PPP1CC 5501
Affinity Capture-MS Homo sapiens
23 AGT 183
Two-hybrid Homo sapiens
24 WAS 7454
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
25 RNPS1 10921
Affinity Capture-MS Homo sapiens
26 RNF20 56254
Two-hybrid Homo sapiens
27 SEMA6A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 NR2C2  
Two-hybrid Homo sapiens
29 TM9SF4 9777
Affinity Capture-MS Homo sapiens
30 ERC1 23085
Two-hybrid Homo sapiens
31 KHDRBS1 10657
Protein-peptide Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
32 RABEP1 9135
Two-hybrid Homo sapiens
33 EPS15L1 58513
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 WASL 8976
Two-hybrid Homo sapiens
35 ATG16L1 55054
Affinity Capture-MS Homo sapiens
36 GAREML  
Two-hybrid Homo sapiens
37 CBLB 868
Affinity Capture-MS Homo sapiens
38 GPNMB 10457
Two-hybrid Homo sapiens
39 GOLGA8A 23015
Two-hybrid Homo sapiens
40 WIPF1 7456
Two-hybrid Homo sapiens
41 RBMX 27316
Two-hybrid Homo sapiens
42 DLGAP1  
Two-hybrid Homo sapiens
43 RNF41  
Affinity Capture-MS Homo sapiens
44 FASLG 356
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
45 RIN3  
Affinity Capture-MS Homo sapiens
46 NCKIPSD 51517
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 WASF2 10163
Two-hybrid Homo sapiens
48 RUFY1 80230
Two-hybrid Homo sapiens
49 CCDC88C 440193
Two-hybrid Homo sapiens
50 NBR1 4077
Two-hybrid Homo sapiens
51 SMEK1 55671
Affinity Capture-MS Homo sapiens
52 PPIL4  
Affinity Capture-MS Homo sapiens
53 CBL 867
Two-hybrid Homo sapiens
54 GOLGA2 2801
Two-hybrid Homo sapiens
55 DIABLO 56616
Proximity Label-MS Homo sapiens
56 NOL7  
Affinity Capture-MS Homo sapiens
57 AP1B1 162
Affinity Capture-MS Homo sapiens
58 TACC1 6867
Two-hybrid Homo sapiens
59 PABPN1 8106
Affinity Capture-MS Homo sapiens
60 ITPKA  
Two-hybrid Homo sapiens
61 DNM2 1785
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
62 GAREM  
Affinity Capture-MS Homo sapiens
63 SNAP29 9342
Two-hybrid Homo sapiens
64 SNRPB2 6629
Affinity Capture-MS Homo sapiens
65 TMF1 7110
Two-hybrid Homo sapiens
66 DST 667
Two-hybrid Homo sapiens
67 LSM8 51691
Two-hybrid Homo sapiens
68 PSEN1 5663
Two-hybrid Homo sapiens
69 SYNJ2 8871
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
70 AHDC1  
Two-hybrid Homo sapiens
71 Pten  
Affinity Capture-MS Mus musculus
72 MRPL9 65005
Affinity Capture-MS Homo sapiens
73 GOLT1B 51026
Affinity Capture-MS Homo sapiens
74 TRIO 7204
Two-hybrid Homo sapiens
75 NR3C1 2908
Proximity Label-MS Homo sapiens
76 YLPM1 56252
Two-hybrid Homo sapiens
77 LTBP4 8425
Two-hybrid Homo sapiens
78 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
79 LARP6 55323
Two-hybrid Homo sapiens
80 PIK3C2B 5287
Two-hybrid Homo sapiens
81 PSMD7 5713
Affinity Capture-MS Homo sapiens
82 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 AMPH 273
Two-hybrid Homo sapiens
84 MYO6 4646
Proximity Label-MS Homo sapiens
85 SOS2 6655
Two-hybrid Homo sapiens
86 FCHSD2  
Two-hybrid Homo sapiens
87 BICD1 636
Proximity Label-MS Homo sapiens
88 SGIP1  
Affinity Capture-MS Homo sapiens
89 STX4 6810
Two-hybrid Homo sapiens
90 GOLGB1 2804
Two-hybrid Homo sapiens
91 FNBP4  
Two-hybrid Homo sapiens
92 KIAA1549  
Two-hybrid Homo sapiens
93 ITSN1 6453
PCA Homo sapiens
94 ROCK1 6093
Two-hybrid Homo sapiens
95 CPSF6 11052
Two-hybrid Homo sapiens
96 PDCD6IP 10015
Two-hybrid Homo sapiens
97 Fcho2  
Affinity Capture-MS Mus musculus
98 Itsn2  
Affinity Capture-MS Mus musculus
99 SOS1 6654
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
100 TBL3 10607
Two-hybrid Homo sapiens
101 HNRNPK 3190
Two-hybrid Homo sapiens
102 CHIC2 26511
Two-hybrid Homo sapiens
103 MBNL1 4154
Two-hybrid Homo sapiens
104 ITSN2 50618
PCA Homo sapiens
PCA Homo sapiens
105 KDM1A 23028
Two-hybrid Homo sapiens
106 PIK3AP1  
Two-hybrid Homo sapiens
107 EZH2  
Affinity Capture-MS Homo sapiens
108 REPS1 85021
Two-hybrid Homo sapiens
109 PCCB 5096
Co-fractionation Homo sapiens
110 CLTA 1211
Proximity Label-MS Homo sapiens
111 KXD1 79036
Two-hybrid Homo sapiens
112 BCCIP 56647
Two-hybrid Homo sapiens
113 EPHA2 1969
Proximity Label-MS Homo sapiens
114 SRGAP2 23380
Affinity Capture-MS Homo sapiens
115 PDE4DIP 9659
Two-hybrid Homo sapiens
116 SH3KBP1 30011
Two-hybrid Homo sapiens
117 SYN1  
Two-hybrid Homo sapiens
118 CCR1  
Affinity Capture-MS Homo sapiens
119 NUP50 10762
Affinity Capture-MS Homo sapiens
120 FCHSD1 89848
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
121 LUC7L3 51747
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here