Gene description for MYO6
Gene name myosin VI
Gene symbol MYO6
Other names/aliases DFNA22
DFNB37
Species Homo sapiens
 Database cross references - MYO6
ExoCarta ExoCarta_4646
Entrez Gene 4646
HGNC 7605
MIM 600970
UniProt Q9UM54  
 MYO6 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Neuroblastoma cells 25944692    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for MYO6
Molecular Function
    motor activity GO:0003774 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    minus-end directed microfilament motor activity GO:0060001 NAS
    actin filament binding GO:0051015 ISS
    ADP binding GO:0043531 ISS
    actin binding GO:0003779 TAS
    calmodulin binding GO:0005516 TAS
Biological Process
    actin filament-based movement GO:0030048 NAS
    regulation of synaptic plasticity GO:0048167 IEA
    inner ear morphogenesis GO:0042472 IEA
    synaptic transmission GO:0007268 TAS
    DNA damage response, signal transduction by p53 class mediator GO:0030330 IDA
    endocytosis GO:0006897 ISS
    metabolic process GO:0008152 NAS
    regulation of secretion GO:0051046 IMP
    locomotory behavior GO:0007626 IEA
    sensory perception of sound GO:0007605 IEA
    glutamate secretion GO:0014047 IEA
    synapse assembly GO:0007416 IEA
    intracellular protein transport GO:0006886 ISS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    protein targeting GO:0006605 IEA
    membrane organization GO:0061024 TAS
    auditory receptor cell differentiation GO:0042491 IEA
    dendrite development GO:0016358 IEA
    response to drug GO:0042493 IEA
    cellular response to electrical stimulus GO:0071257 IEA
Subcellular Localization
    membrane GO:0016020 IDA
    filamentous actin GO:0031941 ISS
    nucleus GO:0005634 IDA
    perinuclear region of cytoplasm GO:0048471 ISS
    clathrin-coated endocytic vesicle GO:0045334 IDA
    unconventional myosin complex GO:0016461 TAS
    axon GO:0030424 IEA
    nuclear membrane GO:0031965 IDA
    coated pit GO:0005905 IEA
    ruffle GO:0001726 IDA
    cytoplasmic membrane-bounded vesicle GO:0016023 IDA
    extracellular exosome GO:0070062 IDA
    DNA-directed RNA polymerase II, holoenzyme GO:0016591 IDA
    endocytic vesicle GO:0030139 ISS
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 TAS
    apical part of cell GO:0045177 IEA
    ruffle membrane GO:0032587 IEA
    neuronal cell body GO:0043025 IEA
    Golgi apparatus GO:0005794 IDA
    cell cortex GO:0005938 ISS
    clathrin-coated vesicle membrane GO:0030665 IEA
    lysosomal membrane GO:0005765 TAS
    nucleoplasm GO:0005654 IDA
    plasma membrane GO:0005886 TAS
    microvillus GO:0005902 IEA
 Experiment description of studies that identified MYO6 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MYO6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DLG1 1739
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 DAB2 1601
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
3 CALM2 805
Two-hybrid Homo sapiens
4 GIPC1 10755
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 DAB1 1600
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which MYO6 is involved
PathwayEvidenceSource
Gap junction degradation TAS Reactome
Trafficking of AMPA receptors TAS Reactome





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