Gene description for SYNJ1
Gene name synaptojanin 1
Gene symbol SYNJ1
Other names/aliases INPP5G
PARK20
Species Homo sapiens
 Database cross references - SYNJ1
ExoCarta ExoCarta_8867
Vesiclepedia VP_8867
Entrez Gene 8867
HGNC 11503
MIM 604297
UniProt O43426  
 SYNJ1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SYNJ1
Molecular Function
    RNA binding GO:0003723 IEA
    phosphatidylinositol-3-phosphate phosphatase activity GO:0004438 IDA
    phosphatidylinositol-3-phosphate phosphatase activity GO:0004438 TAS
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 IBA
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 IDA
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 IMP
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 ISS
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 TAS
    protein binding GO:0005515 IPI
    phosphatidylinositol phosphate 5-phosphatase activity GO:0034595 ISS
    phosphatidylinositol phosphate 4-phosphatase activity GO:0034596 TAS
    phosphatidylinositol-4-phosphate phosphatase activity GO:0043812 IDA
    phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity GO:0043813 TAS
    phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity GO:0052629 TAS
    inositol-1,4,5-trisphosphate 5-phosphatase activity GO:0052658 IBA
    inositol-1,4,5-trisphosphate 5-phosphatase activity GO:0052658 TAS
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    neurotransmitter transport GO:0006836 ISS
    learning GO:0007612 IMP
    synaptic vesicle priming GO:0016082 ISS
    synaptic vesicle uncoating GO:0016191 ISS
    phosphatidylinositol-3-phosphate biosynthetic process GO:0036092 IEA
    inositol phosphate metabolic process GO:0043647 TAS
    phosphatidylinositol metabolic process GO:0046488 ISS
    phosphatidylinositol dephosphorylation GO:0046856 IDA
    phosphatidylinositol dephosphorylation GO:0046856 IMP
    phosphatidylinositol dephosphorylation GO:0046856 ISS
    synaptic vesicle endocytosis GO:0048488 IBA
    synaptic vesicle endocytosis GO:0048488 IGI
    synaptic vesicle endocytosis GO:0048488 ISS
    synaptic vesicle transport GO:0048489 ISS
    membrane organization GO:0061024 TAS
    positive regulation of endosome organization GO:1904980 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 ISS
    vesicle membrane GO:0012506 ISS
    membrane GO:0016020 IBA
    membrane coat GO:0030117 ISS
    clathrin coat of coated pit GO:0030132 ISS
    terminal bouton GO:0043195 ISS
    perinuclear region of cytoplasm GO:0048471 IBA
    perinuclear region of cytoplasm GO:0048471 ISS
    synaptic membrane GO:0097060 ISS
    presynapse GO:0098793 IBA
    presynapse GO:0098793 IDA
 Experiment description of studies that identified SYNJ1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SYNJ1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GPM6A 2823
Affinity Capture-MS Homo sapiens
2 GTSE1 51512
Affinity Capture-MS Homo sapiens
3 SNX27 81609
Affinity Capture-MS Homo sapiens
4 AP2B1 163
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ANKFY1 51479
Proximity Label-MS Homo sapiens
6 HOXA2  
Affinity Capture-MS Homo sapiens
7 ITSN1 6453
Affinity Capture-Western Homo sapiens
8 ALKBH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 PACSIN1  
Reconstituted Complex Homo sapiens
10 LAMTOR1 55004
Proximity Label-MS Homo sapiens
11 ATG9A 79065
Proximity Label-MS Homo sapiens
12 RBM47 54502
Affinity Capture-MS Homo sapiens
13 SH3GL1 6455
Protein-peptide Homo sapiens
14 PFN1 5216
Proximity Label-MS Homo sapiens
15 KRT8 3856
Proximity Label-MS Homo sapiens
16 ADAP1  
Co-fractionation Homo sapiens
17 EZR 7430
Proximity Label-MS Homo sapiens
18 ACTC1 70
Proximity Label-MS Homo sapiens
19 PACSIN2 11252
Reconstituted Complex Homo sapiens
20 LCK 3932
Proximity Label-MS Homo sapiens
21 DYNC1I1  
Proximity Label-MS Homo sapiens
22 SQSTM1 8878
Proximity Label-MS Homo sapiens
23 PRPH 5630
Proximity Label-MS Homo sapiens
24 PACSIN3 29763
Reconstituted Complex Homo sapiens
25 CLTB 1212
Proximity Label-MS Homo sapiens
26 MAGEA9  
Affinity Capture-MS Homo sapiens
27 SNX9 51429
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
29 AMPH 273
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
30 C10orf88  
Affinity Capture-MS Homo sapiens
31 LRRK2 120892
Biochemical Activity Homo sapiens
32 CDH1 999
Proximity Label-MS Homo sapiens
33 MYO6 4646
Proximity Label-MS Homo sapiens
34 IMPDH2 3615
Proximity Label-MS Homo sapiens
35 DYNC1LI1 51143
Proximity Label-MS Homo sapiens
36 DYNC1LI2 1783
Proximity Label-MS Homo sapiens
37 CLTA 1211
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
38 AP2A1 160
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
39 AP1B1 162
Affinity Capture-MS Homo sapiens
40 MKRN2 23609
Affinity Capture-RNA Homo sapiens
41 SORBS3 10174
Affinity Capture-MS Homo sapiens
42 BICD2 23299
Proximity Label-MS Homo sapiens
43 BICD1 636
Proximity Label-MS Homo sapiens
44 KRAS 3845
Proximity Label-MS Homo sapiens
45 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-MS Homo sapiens
46 CLTC 1213
Reconstituted Complex Homo sapiens
47 SH3GL2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
48 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 STX6 10228
Proximity Label-MS Homo sapiens
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