Gene description for SYNJ1
Gene name synaptojanin 1
Gene symbol SYNJ1
Other names/aliases INPP5G
PARK20
Species Homo sapiens
 Database cross references - SYNJ1
ExoCarta ExoCarta_8867
Vesiclepedia VP_8867
Entrez Gene 8867
HGNC 11503
MIM 604297
UniProt O43426  
 SYNJ1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SYNJ1
Molecular Function
    phosphatidylinositol phosphate 5-phosphatase activity GO:0034595 ISS
    inositol-polyphosphate 5-phosphatase activity GO:0004445 TAS
    nucleotide binding GO:0000166 IEA
    RNA binding GO:0003723 IEA
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 ISS
Biological Process
    synaptic vesicle uncoating GO:0016191 ISS
    small molecule metabolic process GO:0044281 TAS
    positive regulation of synaptic vesicle uncoating GO:1903390 TAS
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    synaptic vesicle endocytosis GO:0048488 ISS
    neurotransmitter transport GO:0006836 ISS
    synaptic vesicle transport GO:0048489 ISS
    phospholipid metabolic process GO:0006644 TAS
    inositol phosphate metabolic process GO:0043647 TAS
    synaptic vesicle priming GO:0016082 ISS
    phosphatidylinositol metabolic process GO:0046488 ISS
    phosphatidylinositol dephosphorylation GO:0046856 ISS
    inositol phosphate dephosphorylation GO:0046855 ISS
    phosphate-containing compound metabolic process GO:0006796 TAS
    regulation of synaptic vesicle membrane organization GO:1901632 TAS
Subcellular Localization
    membrane coat GO:0030117 ISS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified SYNJ1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SYNJ1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AMPH 273
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
3 PACSIN2 11252
Reconstituted Complex Homo sapiens
4 EPS15 2060
Invitro Homo sapiens
5 BIN1 274
Invitro Homo sapiens
6 PACSIN1  
Reconstituted Complex Homo sapiens
7 SH3GL2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 PACSIN3 29763
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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