Experiment description of studies that identified PRPH in exosomes

1 Experiment ID 267 MISEV standards ✘ Biophysical techniques ✔ TSG101|Alix Enriched markers ✔ CANX|HSP90B1 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25265333     Organism Homo sapiens Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J" Journal name J Proteome Res Publication year 2014 Sample Liver cancer cells Sample name Huh-7 - Transfected with HBx plasmids Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for PRPH

Protein Interactor ExoCarta ID Identification method PubMed Species 1 KRT76 51350 Cross-Linking-MS (XL-MS) Homo sapiens 2 KRT77 374454 Cross-Linking-MS (XL-MS) Homo sapiens 3 ARHGAP21 57584 Proximity Label-MS Homo sapiens 4 C1orf198 84886 Proximity Label-MS Homo sapiens 5 C1orf86   Proximity Label-MS Homo sapiens 6 ABLIM1 3983 Proximity Label-MS Homo sapiens 7 RASSF8 11228 Proximity Label-MS Homo sapiens 8 SPRTN   Affinity Capture-MS Homo sapiens 9 KRT6B 3854 Cross-Linking-MS (XL-MS) Homo sapiens 10 KRT1 3848 Cross-Linking-MS (XL-MS) Homo sapiens 11 FAM167A   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 IPMK   Proximity Label-MS Homo sapiens 13 LDHB 3945 Proximity Label-MS Homo sapiens 14 KIFC3 3801 Two-hybrid Homo sapiens 15 KRT6A 3853 Cross-Linking-MS (XL-MS) Homo sapiens 16 KRT17 3872 Cross-Linking-MS (XL-MS) Homo sapiens 17 CRK 1398 Proximity Label-MS Homo sapiens 18 GFAP 2670 Proximity Label-MS Homo sapiens 19 FCHO1   Two-hybrid Homo sapiens 20 IRAK1   Proximity Label-MS Homo sapiens 21 KIF20A 10112 Affinity Capture-MS Homo sapiens 22 CEP41   Proximity Label-MS Homo sapiens 23 NENF 29937 Co-fractionation Homo sapiens 24 TTC23   Two-hybrid Homo sapiens 25 KIF7 374654 Proximity Label-MS Homo sapiens 26 KIF1B 23095 Proximity Label-MS Homo sapiens 27 SNX6 58533 Proximity Label-MS Homo sapiens 28 TOMM22 56993 Proximity Label-MS Homo sapiens 29 ZMAT2   Two-hybrid Homo sapiens 30 CKB 1152 Proximity Label-MS Homo sapiens 31 INA 9118 Proximity Label-MS Homo sapiens 32 TNRC6A 27327 Proximity Label-MS Homo sapiens 33 SIRT2 22933 Proximity Label-MS Homo sapiens 34 ARHGAP32   Proximity Label-MS Homo sapiens 35 KIF23 9493 Affinity Capture-MS Homo sapiens 36 CFAP97   Proximity Label-MS Homo sapiens 37 LENG1   Two-hybrid Homo sapiens 38 HGS 9146 Proximity Label-MS Homo sapiens 39 CDH2 1000 Co-fractionation Homo sapiens 40 PPP1R18 170954 Two-hybrid Homo sapiens 41 FBXW7   Affinity Capture-MS Homo sapiens 42 PRC1 9055 Affinity Capture-MS Homo sapiens 43 MAGOHB 55110 Two-hybrid Homo sapiens 44 PSMD12 5718 Proximity Label-MS Homo sapiens 45 ETFA 2108 Proximity Label-MS Homo sapiens 46 PSMC2 5701 Proximity Label-MS Homo sapiens 47 KRT5 3852 Cross-Linking-MS (XL-MS) Homo sapiens 48 LPP 4026 Proximity Label-MS Homo sapiens 49 KRT72 140807 Cross-Linking-MS (XL-MS) Homo sapiens 50 DDX6 1656 Two-hybrid Homo sapiens 51 RIPK2   Proximity Label-MS Homo sapiens 52 MECP2 4204 Affinity Capture-MS Homo sapiens 53 TFCP2 7024 Affinity Capture-MS Homo sapiens 54 MYCN   Affinity Capture-MS Homo sapiens 55 SDCBP 6386 Proximity Label-MS Homo sapiens 56 SYNM 23336 Proximity Label-MS Homo sapiens 57 SOD1 6647 Affinity Capture-MS Homo sapiens 58 KIF14 9928 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 59 SORBS1 10580 Proximity Label-MS Homo sapiens 60 CCT2 10576 Proximity Label-MS Homo sapiens 61 PDLIM3 27295 Proximity Label-MS Homo sapiens 62 DDX19B 11269 Proximity Label-MS Homo sapiens 63 KRT8 3856 Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens Proximity Label-MS Homo sapiens 64 KIAA1217 56243 Proximity Label-MS Homo sapiens 65 TCP10L   Two-hybrid Homo sapiens 66 C19orf44   Proximity Label-MS Homo sapiens 67 NDUFS4 4724 Co-fractionation Homo sapiens 68 COX15 1355 Affinity Capture-MS Homo sapiens 69 TBK1 29110 Proximity Label-MS Homo sapiens 70 CAPZA1 829 Proximity Label-MS Homo sapiens 71 USP11 8237 Affinity Capture-MS Homo sapiens 72 ALDH1A2 8854 Proximity Label-MS Homo sapiens 73 DRG1 4733 Proximity Label-MS Homo sapiens 74 KRT6C 286887 Cross-Linking-MS (XL-MS) Homo sapiens 75 TCEA2   Two-hybrid Homo sapiens 76 LASP1 3927 Proximity Label-MS Homo sapiens 77 EXOC4 60412 Proximity Label-MS Homo sapiens 78 GOLGA2 2801 Proximity Label-MS Homo sapiens 79 PFKM 5213 Proximity Label-MS Homo sapiens 80 CHMP4B 128866 Affinity Capture-MS Homo sapiens 81 EXPH5   Proximity Label-MS Homo sapiens 82 YAP1 10413 Affinity Capture-MS Homo sapiens 83 CCDC85C   Proximity Label-MS Homo sapiens 84 SYNJ1 8867 Proximity Label-MS Homo sapiens 85 ARFGAP2 84364 Proximity Label-MS Homo sapiens 86 SYNC   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 87 TCHP   Proximity Label-MS Homo sapiens 88 KRT75 9119 Cross-Linking-MS (XL-MS) Homo sapiens Two-hybrid Homo sapiens 89 ZNF572   Two-hybrid Homo sapiens 90 TARS2 80222 Proximity Label-MS Homo sapiens 91 KRT14 3861 Cross-Linking-MS (XL-MS) Homo sapiens Two-hybrid Homo sapiens 92 BICD2 23299 Proximity Label-MS Homo sapiens 93 ENO1 2023 Proximity Label-MS Homo sapiens 94 KRT2 3849 Cross-Linking-MS (XL-MS) Homo sapiens 95 CT55   Two-hybrid Homo sapiens 96 C6orf132   Proximity Label-MS Homo sapiens 97 STUB1 10273 Proximity Label-MS Homo sapiens 98 BAG3 9531 Proximity Label-MS Homo sapiens 99 ANLN 54443 Affinity Capture-MS Homo sapiens 100 GAN 8139 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 101 TCP10   Affinity Capture-MS Homo sapiens 102 KRT3 3850 Cross-Linking-MS (XL-MS) Homo sapiens 103 FAM83B   Proximity Label-MS Homo sapiens 104 PPP2R2B 5521 Affinity Capture-MS Homo sapiens 105 CSTF2T   Proximity Label-MS Homo sapiens 106 TBKBP1   Proximity Label-MS Homo sapiens 107 SDHA 6389 Affinity Capture-MS Homo sapiens 108 ECT2 1894 Affinity Capture-MS Homo sapiens 109 OCRL 4952 Proximity Label-MS Homo sapiens 110 LCA5L   Two-hybrid Homo sapiens 111 CAMSAP3   Proximity Label-MS Homo sapiens 112 PRKAA2 5563 Two-hybrid Homo sapiens 113 TAB2   Proximity Label-MS Homo sapiens 114 ZC4H2 55906 Two-hybrid Homo sapiens 115 CUL3 8452 Affinity Capture-MS Homo sapiens 116 DES 1674 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 117 IFFO1   Proximity Label-MS Homo sapiens 118 USP54 159195 Proximity Label-MS Homo sapiens 119 ROCK2 9475 Proximity Label-MS Homo sapiens 120 HSD17B10 3028 Affinity Capture-MS Homo sapiens 121 VPS13C 54832 Proximity Label-MS Homo sapiens 122 KRT27 342574 Two-hybrid Homo sapiens 123 NXF1 10482 Two-hybrid Homo sapiens 124 SCYL2 55681 Proximity Label-MS Homo sapiens 125 IFFO2 126917 Proximity Label-MS Homo sapiens 126 KRT15 3866 Two-hybrid Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 127 SMOX   Affinity Capture-MS Homo sapiens 128 SMG7   Proximity Label-MS Homo sapiens 129 ZMYM6   Proximity Label-MS Homo sapiens 130 MYC   Affinity Capture-MS Homo sapiens 131 DLST 1743 Affinity Capture-MS Homo sapiens 132 HSPA1A 3303 Proximity Label-MS Homo sapiens 133 SH3D19 152503 Proximity Label-MS Homo sapiens 134 C1D   Affinity Capture-MS Homo sapiens 135 BICD1 636 Proximity Label-MS Homo sapiens 136 KIAA1671   Proximity Label-MS Homo sapiens 137 VDAC1 7416 Affinity Capture-MS Homo sapiens 138 KRT19 3880 Cross-Linking-MS (XL-MS) Homo sapiens Two-hybrid Homo sapiens Proximity Label-MS Homo sapiens 139 HINT1 3094 Proximity Label-MS Homo sapiens 140 KRT16 3868 Cross-Linking-MS (XL-MS) Homo sapiens Two-hybrid Homo sapiens 141 DSP 1832 Proximity Label-MS Homo sapiens 142 MPHOSPH9   Proximity Label-MS Homo sapiens 143 SACS 26278 Proximity Label-MS Homo sapiens 144 NES 10763 Proximity Label-MS Homo sapiens 145 KRT18 3875 Proximity Label-MS Homo sapiens 146 PDLIM7 9260 Proximity Label-MS Homo sapiens 147 TP53BP2   Proximity Label-MS Homo sapiens 148 ILF3 3609 Co-fractionation Homo sapiens 149 KRT84 3890 Cross-Linking-MS (XL-MS) Homo sapiens 150 PLEC 5339 Proximity Label-MS Homo sapiens 151 PDCD2L 84306 Proximity Label-MS Homo sapiens 152 ASNS 440 Proximity Label-MS Homo sapiens 153 DNAJA2 10294 Proximity Label-MS Homo sapiens 154 CALU 813 Co-fractionation Homo sapiens 155 KRT10 3858 Cross-Linking-MS (XL-MS) Homo sapiens 156 INCA1   Two-hybrid Homo sapiens 157 CDC37 11140 Two-hybrid Homo sapiens 158 SIPA1L3   Proximity Label-MS Homo sapiens 159 PDHA1 5160 Affinity Capture-MS Homo sapiens 160 KRT71 112802 Cross-Linking-MS (XL-MS) Homo sapiens 161 NUDT19 390916 Proximity Label-MS Homo sapiens 162 STK38L 23012 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 163 MTHFD1 4522 Co-fractionation Homo sapiens 164 CDK1 983 Proximity Label-MS Homo sapiens 165 PSMA1 5682 Two-hybrid Homo sapiens 166 EIF4H 7458 Proximity Label-MS Homo sapiens 167 STK38 11329 Proximity Label-MS Homo sapiens 168 AAK1 22848 Proximity Label-MS Homo sapiens 169 KRT24 192666 Two-hybrid Homo sapiens 170 PPP1R13B   Proximity Label-MS Homo sapiens 171 KRT20 54474 Two-hybrid Homo sapiens 172 FARSA 2193 Proximity Label-MS Homo sapiens 173 RTCB 51493 Proximity Label-MS Homo sapiens 174 KRT12 3859 Cross-Linking-MS (XL-MS) Homo sapiens 175 LIMD1 8994 Proximity Label-MS Homo sapiens 176 PLEKHG1   Proximity Label-MS Homo sapiens 177 HNRNPU 3192 Co-fractionation Homo sapiens 178 CKS2   Proximity Label-MS Homo sapiens 179 VIM 7431 Two-hybrid Homo sapiens Proximity Label-MS Homo sapiens 180 PRMT1 3276 Affinity Capture-MS Homo sapiens 181 IARS2 55699 Proximity Label-MS Homo sapiens 182 IFT74 80173 Proximity Label-MS Homo sapiens View the network

Pathways in which PRPH is involved

No pathways found