Gene description for KRT76
Gene name keratin 76, type II
Gene symbol KRT76
Other names/aliases HUMCYT2A
KRT2B
KRT2P
Species Homo sapiens
 Database cross references - KRT76
ExoCarta ExoCarta_51350
Vesiclepedia VP_51350
Entrez Gene 51350
HGNC 24430
UniProt Q01546  
 KRT76 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for KRT76
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of skin epidermis GO:0030280 IBA
Biological Process
    cytoskeleton organization GO:0007010 NAS
    keratinization GO:0031424 IBA
    pigmentation GO:0043473 IEA
    intermediate filament organization GO:0045109 IBA
    sebaceous gland development GO:0048733 IEA
Subcellular Localization
    nucleus GO:0005634 HDA
    cytosol GO:0005829 TAS
    intermediate filament GO:0005882 NAS
    keratin filament GO:0045095 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KRT76 in exosomes
1
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KRT76
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 INSIG1  
Affinity Capture-MS Homo sapiens
2 KRT84 3890
Cross-Linking-MS (XL-MS) Homo sapiens
3 KRT37 8688
Two-hybrid Homo sapiens
4 TFCP2 7024
Affinity Capture-MS Homo sapiens
5 KRT27 342574
Two-hybrid Homo sapiens
6 KRT16 3868
Cross-Linking-MS (XL-MS) Homo sapiens
Two-hybrid Homo sapiens
7 LMO4  
Two-hybrid Homo sapiens
8 KRT36 8689
Two-hybrid Homo sapiens
9 KRT25 147183
Two-hybrid Homo sapiens
10 EXOC8 149371
Two-hybrid Homo sapiens
11 FRS3 10817
Two-hybrid Homo sapiens
12 C1orf216  
Two-hybrid Homo sapiens
13 KIFC3 3801
Two-hybrid Homo sapiens
14 INSIG2  
Affinity Capture-MS Homo sapiens
15 KRT86  
Two-hybrid Homo sapiens
16 KRT6A 3853
Cross-Linking-MS (XL-MS) Homo sapiens
17 LCA5L  
Two-hybrid Homo sapiens
18 CCDC57 284001
Two-hybrid Homo sapiens
19 COPS6 10980
Affinity Capture-MS Homo sapiens
20 CRK 1398
Affinity Capture-MS Homo sapiens
21 KRT10 3858
Cross-Linking-MS (XL-MS) Homo sapiens
22 TRIM27  
Two-hybrid Homo sapiens
23 WASH1 100287171
Two-hybrid Homo sapiens
24 CDC37 11140
Two-hybrid Homo sapiens
25 CUL3 8452
Affinity Capture-MS Homo sapiens
26 DES 1674
Cross-Linking-MS (XL-MS) Homo sapiens
27 KRT39  
Two-hybrid Homo sapiens
28 PRPH 5630
Cross-Linking-MS (XL-MS) Homo sapiens
29 CAND1 55832
Affinity Capture-MS Homo sapiens
30 RNF208  
Affinity Capture-MS Homo sapiens
31 TRIM54  
Two-hybrid Homo sapiens
32 DPPA3  
Two-hybrid Homo sapiens
33 CUL4B 8450
Affinity Capture-MS Homo sapiens
34 NFKBID  
Two-hybrid Homo sapiens
35 KRT15 3866
Cross-Linking-MS (XL-MS) Homo sapiens
36 ZNF655  
Two-hybrid Homo sapiens
37 KRT17 3872
Cross-Linking-MS (XL-MS) Homo sapiens
38 KRT19 3880
Cross-Linking-MS (XL-MS) Homo sapiens
39 KRT20 54474
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
40 KRT38 8687
Two-hybrid Homo sapiens
41 POLR1C 9533
Two-hybrid Homo sapiens
42 TRIM23 373
Two-hybrid Homo sapiens
43 KRT12 3859
Cross-Linking-MS (XL-MS) Homo sapiens
44 C1orf109  
Two-hybrid Homo sapiens
45 KRT75 9119
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
46 NEDD8 4738
Affinity Capture-MS Homo sapiens
47 IQUB  
Two-hybrid Homo sapiens
48 TNS2 23371
Two-hybrid Homo sapiens
49 COPS5 10987
Affinity Capture-MS Homo sapiens
50 KRT40  
Two-hybrid Homo sapiens
51 HGS 9146
Two-hybrid Homo sapiens
52 FOXR2  
Two-hybrid Homo sapiens
53 CUL2 8453
Affinity Capture-MS Homo sapiens
54 VIM 7431
Cross-Linking-MS (XL-MS) Homo sapiens
55 MTUS2 23281
Two-hybrid Homo sapiens
56 KRT5 3852
Cross-Linking-MS (XL-MS) Homo sapiens
57 YAP1 10413
Affinity Capture-MS Homo sapiens
58 KRT7 3855
Cross-Linking-MS (XL-MS) Homo sapiens
59 TSGA10  
Two-hybrid Homo sapiens
60 CUL4A 8451
Affinity Capture-MS Homo sapiens
61 KRT14 3861
Cross-Linking-MS (XL-MS) Homo sapiens
62 C6orf165  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which KRT76 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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