Gene description for KRT15
Gene name keratin 15, type I
Gene symbol KRT15
Other names/aliases CK15
K15
K1CO
Species Homo sapiens
 Database cross references - KRT15
ExoCarta ExoCarta_3866
Vesiclepedia VP_3866
Entrez Gene 3866
HGNC 6421
MIM 148030
UniProt P19012  
 KRT15 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Saliva 19199708    
Squamous carcinoma cells 20124223    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for KRT15
Molecular Function
    structural constituent of cytoskeleton GO:0005200 TAS
    protein binding GO:0005515 IPI
    scaffold protein binding GO:0097110 IPI
Biological Process
    epidermis development GO:0008544 TAS
    epithelial cell differentiation GO:0030855 IBA
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    nucleus GO:0005634 HDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IBA
    intermediate filament GO:0005882 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KRT15 in exosomes
1
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
4
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
6
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
7
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
8
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KRT15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MORN3  
Two-hybrid Homo sapiens
2 KRT77 374454
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 KRT4 3851
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 KRT73 319101
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 KRT76 51350
Cross-Linking-MS (XL-MS) Homo sapiens
6 KRT6B 3854
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Two-hybrid Homo sapiens
7 KRT1 3848
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 KIFC3 3801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 KRT6A 3853
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Two-hybrid Homo sapiens
10 KLC3  
Two-hybrid Homo sapiens
11 CRK 1398
Affinity Capture-MS Homo sapiens
12 APC  
Two-hybrid Homo sapiens
13 GFAP 2670
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 TSG101 7251
Two-hybrid Homo sapiens
15 SMARCE1 6605
Two-hybrid Homo sapiens
16 ENKD1  
Two-hybrid Homo sapiens
17 GAS8  
Two-hybrid Homo sapiens
18 TTC23  
Two-hybrid Homo sapiens
19 CCNB1IP1  
Two-hybrid Homo sapiens
20 RNF208  
Affinity Capture-MS Homo sapiens
21 HAPLN2  
Two-hybrid Homo sapiens
22 HOXB6 3216
Two-hybrid Homo sapiens
23 TELO2 9894
Two-hybrid Homo sapiens
24 LMO2  
Two-hybrid Homo sapiens
25 KRT71 112802
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
26 TIMM8A 1678
Two-hybrid Homo sapiens
27 ABLIM1 3983
Two-hybrid Homo sapiens
28 ZNF638 27332
Two-hybrid Homo sapiens
29 PPP1R18 170954
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 HGS 9146
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 ZGPAT  
Two-hybrid Homo sapiens
32 OIP5  
Two-hybrid Homo sapiens
33 KRT5 3852
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Two-hybrid Homo sapiens
34 KRT78 196374
Two-hybrid Homo sapiens
35 ATP5O 539
Two-hybrid Homo sapiens
36 HMG20B  
Two-hybrid Homo sapiens
37 KRT72 140807
Two-hybrid Homo sapiens
38 KRT83  
Two-hybrid Homo sapiens
39 ZNF576  
Two-hybrid Homo sapiens
40 TUBGCP4 27229
Two-hybrid Homo sapiens
41 USP2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
42 TFCP2 7024
Affinity Capture-MS Homo sapiens
43 CREB5 9586
Two-hybrid Homo sapiens
44 MGC50722  
Two-hybrid Homo sapiens
45 CCNC  
Two-hybrid Homo sapiens
46 AMOT  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
47 NIF3L1 60491
Two-hybrid Homo sapiens
48 LMO4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
49 C1orf216  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
50 KANSL1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
51 KRT8 3856
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
52 KIAA1217 56243
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
53 SPG21 51324
Two-hybrid Homo sapiens
54 BEX2  
Two-hybrid Homo sapiens
55 TRIML2  
Two-hybrid Homo sapiens
56 IKBIP 121457
Two-hybrid Homo sapiens
57 PRPH 5630
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
58 MOS 4342
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
59 FAM110A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
60 NUDT18  
Two-hybrid Homo sapiens
61 KLC4 89953
Two-hybrid Homo sapiens
62 TRIM42  
Two-hybrid Homo sapiens
63 AES 166
Two-hybrid Homo sapiens
64 ZNF417  
Two-hybrid Homo sapiens
65 FAM107A  
Two-hybrid Homo sapiens
66 MCM10 55388
Two-hybrid Homo sapiens
67 KRT80 144501
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
68 DTNB 1838
Two-hybrid Homo sapiens
69 NOC4L 79050
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
70 KRT75 9119
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
71 PKN1 5585
Two-hybrid Homo sapiens
72 CCDC101  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
73 KRT2 3849
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
74 MBD3 53615
Two-hybrid Homo sapiens
75 YAP1 10413
Affinity Capture-MS Homo sapiens
76 PRPF31 26121
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
77 CEP57  
Two-hybrid Homo sapiens
78 TXNDC11 51061
Two-hybrid Homo sapiens
79 SSX2IP  
Two-hybrid Homo sapiens
80 EFHC1  
Two-hybrid Homo sapiens
81 KRT85  
Two-hybrid Homo sapiens
82 TXLNA 200081
Two-hybrid Homo sapiens
83 ABI3 51225
Two-hybrid Homo sapiens
84 DGCR6L  
Two-hybrid Homo sapiens
85 COX5B 1329
Two-hybrid Homo sapiens
86 CCDC185  
Two-hybrid Homo sapiens
87 SMARCD1 6602
Two-hybrid Homo sapiens
88 KRT86  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
89 CCDC120  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
90 TCHP  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
91 TEKT4  
Two-hybrid Homo sapiens
92 CALCOCO1 57658
Two-hybrid Homo sapiens
93 TXLNB  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
94 SIRPA 140885
Two-hybrid Homo sapiens
95 DES 1674
Cross-Linking-MS (XL-MS) Homo sapiens
Two-hybrid Homo sapiens
96 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
97 KRT82 3888
Two-hybrid Homo sapiens
98 C10orf10  
Two-hybrid Homo sapiens
99 RAD51D  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
100 KRT81  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
101 RPP25  
Two-hybrid Homo sapiens
102 NEFL 4747
Two-hybrid Homo sapiens
103 RIBC1  
Two-hybrid Homo sapiens
104 GLYCTK  
Two-hybrid Homo sapiens
105 ARC  
Two-hybrid Homo sapiens
106 RHPN1  
Two-hybrid Homo sapiens
107 PSMC5 5705
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
108 RIBC2  
Two-hybrid Homo sapiens
109 KRT84 3890
Cross-Linking-MS (XL-MS) Homo sapiens
110 USHBP1  
Two-hybrid Homo sapiens
111 PCM1 5108
Two-hybrid Homo sapiens
112 RCOR3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
113 ZC2HC1C  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
114 KRT19 3880
Cross-Linking-MS (XL-MS) Homo sapiens
Two-hybrid Homo sapiens
115 PSMB1 5689
Two-hybrid Homo sapiens
116 WAC  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
117 ALS2CR11  
Two-hybrid Homo sapiens
118 FAM103A1  
Two-hybrid Homo sapiens
119 ANKRD36BP1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
120 KRT18 3875
Two-hybrid Homo sapiens
121 MAD2L1BP  
Two-hybrid Homo sapiens
122 NUP54 53371
Two-hybrid Homo sapiens
123 GRB2 2885
Affinity Capture-MS Homo sapiens
124 HAUS1  
Two-hybrid Homo sapiens
125 EXOC8 149371
Two-hybrid Homo sapiens
126 TBC1D21 161514
Two-hybrid Homo sapiens
127 CCHCR1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
128 CDK18 5129
Two-hybrid Homo sapiens
129 CYTH4  
Two-hybrid Homo sapiens
130 EPC1  
Two-hybrid Homo sapiens
131 SNAPIN 23557
Two-hybrid Homo sapiens
132 ARFIP2 23647
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
133 MKI67  
Affinity Capture-MS Homo sapiens
134 PSMA1 5682
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
135 KRT79 338785
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
136 CARD9 64170
Two-hybrid Homo sapiens
137 KRT6C 286887
Two-hybrid Homo sapiens
138 ABI2 10152
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
139 KRT20 54474
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
140 KRT74 121391
Two-hybrid Homo sapiens
141 AMOTL2 51421
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
142 LDOC1  
Two-hybrid Homo sapiens
143 XRCC6 2547
Proximity Label-MS Homo sapiens
144 PIK3R2 5296
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
145 TMSB4X 7114
Two-hybrid Homo sapiens
146 CCDC87  
Two-hybrid Homo sapiens
147 CCDC67  
Two-hybrid Homo sapiens
148 VIM 7431
Cross-Linking-MS (XL-MS) Homo sapiens
149 ZFYVE26 23503
Two-hybrid Homo sapiens
150 KIAA0408  
Two-hybrid Homo sapiens
151 KRT7 3855
Cross-Linking-MS (XL-MS) Homo sapiens
152 LNX1  
Two-hybrid Homo sapiens
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