Gene description for SMARCE1
Gene name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Gene symbol SMARCE1
Other names/aliases BAF57
Species Homo sapiens
 Database cross references - SMARCE1
ExoCarta ExoCarta_6605
Entrez Gene 6605
HGNC 11109
MIM 603111
UniProt Q969G3  
 SMARCE1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for SMARCE1
Molecular Function
    chromatin binding GO:0003682 TAS
    protein N-terminus binding GO:0047485 IPI
    RNA binding GO:0003723 IEA
    RNA polymerase II distal enhancer sequence-specific DNA binding GO:0000980 IDA
    ligand-dependent nuclear receptor binding GO:0016922 IPI
    nucleosomal DNA binding GO:0031492 IDA
    protein binding GO:0005515 IPI
    N-acetyltransferase activity GO:0008080 IDA
    transcription coactivator activity GO:0003713 NAS
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IDA
Biological Process
    ATP-dependent chromatin remodeling GO:0043044 IDA
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    metabolic process GO:0008152 IDA
    nervous system development GO:0007399 IEA
    chromatin remodeling GO:0006338 IDA
    regulation of transcription from RNA polymerase II promoter GO:0006357 NAS
    nucleosome disassembly GO:0006337 IDA
Subcellular Localization
    nBAF complex GO:0071565 ISS
    transcriptional repressor complex GO:0017053 IPI
    nuclear chromosome GO:0000228 TAS
    nuclear chromatin GO:0000790 IDA
    SWI/SNF complex GO:0016514 IDA
    npBAF complex GO:0071564 ISS
    nucleus GO:0005634 IDA
    protein complex GO:0043234 IDA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified SMARCE1 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SMARCE1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GATA1  
Reconstituted Complex Homo sapiens
2 ESR1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 TFE3  
Reconstituted Complex Homo sapiens
4 ACTB 60
Affinity Capture-Western Homo sapiens
5 SMARCA2  
Co-purification Homo sapiens
6 NCOA1  
Reconstituted Complex Homo sapiens
7 ESR2  
Reconstituted Complex Homo sapiens
8 SMARCA4 6597
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 KLF1  
Reconstituted Complex Homo sapiens
10 NR3C1 2908
Affinity Capture-Western Homo sapiens
11 SMARCB1 6598
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
12 STAT2 6773
Affinity Capture-Western Homo sapiens
13 ACTL6A 86
Affinity Capture-Western Homo sapiens
14 Esr1  
Reconstituted Complex Mus musculus
15 SMARCC1 6599
Affinity Capture-Western Homo sapiens
16 BAZ1B 9031
Affinity Capture-MS Homo sapiens
17 RELB  
Affinity Capture-MS Homo sapiens
18 SMARCC2 6601
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SMARCE1 is involved
PathwayEvidenceSource
RMTs methylate histone arginines TAS Reactome





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