Gene description for SMARCC2
Gene name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
Gene symbol SMARCC2
Other names/aliases BAF170
CRACC2
Rsc8
Species Homo sapiens
 Database cross references - SMARCC2
ExoCarta ExoCarta_6601
Entrez Gene 6601
HGNC 11105
MIM 601734
UniProt Q8TAQ2  
 SMARCC2 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for SMARCC2
Molecular Function
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IDA
    RNA polymerase II distal enhancer sequence-specific DNA binding GO:0000980 IDA
    nucleosomal DNA binding GO:0031492 IDA
    transcription coactivator activity GO:0003713 NAS
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    regulation of transcription from RNA polymerase II promoter GO:0006357 NAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    chromatin organization GO:0006325 TAS
    transcription, DNA-templated GO:0006351 IEA
    ATP-dependent chromatin remodeling GO:0043044 IDA
    nucleosome disassembly GO:0006337 IDA
    chromatin remodeling GO:0006338 IDA
    regulation of transcription from RNA polymerase II promoter involved in forebrain neuron fate commitment GO:0021882 IEA
Subcellular Localization
    protein complex GO:0043234 IDA
    SWI/SNF complex GO:0016514 IDA
    transcriptional repressor complex GO:0017053 IPI
    nBAF complex GO:0071565 ISS
    nuclear chromatin GO:0000790 IDA
    npBAF complex GO:0071564 ISS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified SMARCC2 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SMARCC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GATA1  
Reconstituted Complex Homo sapiens
2 POLR2C 5432
Two-hybrid Homo sapiens
3 PEX14  
Two-hybrid Homo sapiens
4 SMARCB1 6598
Co-purification Homo sapiens
5 ITSN1 6453
Two-hybrid Homo sapiens
6 STAT2 6773
Affinity Capture-Western Homo sapiens
7 KLF1  
Reconstituted Complex Homo sapiens
8 BAZ1B 9031
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
9 SMARCD1 6602
Affinity Capture-Western Homo sapiens
10 SP1  
Reconstituted Complex Homo sapiens
11 BRCA1 672
Co-purification Homo sapiens
12 SMARCE1 6605
Affinity Capture-Western Homo sapiens
13 ARRB2  
Two-hybrid Homo sapiens
14 HSP90B1 7184
Affinity Capture-Western Homo sapiens
15 SMARCA2  
Co-purification Homo sapiens
16 SMARCA4 6597
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 NCOR1  
Co-purification Homo sapiens
18 PHYHIP 9796
Two-hybrid Homo sapiens
19 CDK8  
Co-fractionation Homo sapiens
20 ING1  
Affinity Capture-MS Homo sapiens
21 RELB  
Affinity Capture-MS Homo sapiens
22 ACTB 60
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SMARCC2 is involved
PathwayEvidenceSource
RMTs methylate histone arginines TAS Reactome





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