Gene description for LMNB1
Gene name lamin B1
Gene symbol LMNB1
Other names/aliases ADLD
LMN
LMN2
LMNB
Species Homo sapiens
 Database cross references - LMNB1
ExoCarta ExoCarta_4001
Entrez Gene 4001
HGNC 6637
MIM 150340
UniProt P20700  
 LMNB1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for LMNB1
Molecular Function
    structural molecule activity GO:0005198 IEA
    phospholipase binding GO:0043274 IEA
Biological Process
    apoptotic process GO:0006915 TAS
    programmed cell death GO:0012501 TAS
    cellular component disassembly involved in execution phase of apoptosis GO:0006921 TAS
Subcellular Localization
    lamin filament GO:0005638 IEA
    nuclear envelope GO:0005635 TAS
    nuclear membrane GO:0031965 IDA
    nuclear inner membrane GO:0005637 IEA
    nucleoplasm GO:0005654 TAS
    membrane GO:0016020 IDA
 Experiment description of studies that identified LMNB1 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LMNB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDK1 983
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
2 TMPO 7112
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 LBR 3930
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
4 PRPH 5630
Affinity Capture-MS Homo sapiens
5 PRKCD 5580
Invivo Homo sapiens
6 PRKCB 5579
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
7 EMD  
Affinity Capture-Western Homo sapiens
8 LMNA 4000
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
9 PRKCA 5578
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
10 CASP3 836
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
11 CASP6 839
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
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