Gene description for CRNKL1
Gene name crooked neck pre-mRNA splicing factor 1
Gene symbol CRNKL1
Other names/aliases CLF
CRN
Clf1
HCRN
MSTP021
SYF3
Species Homo sapiens
 Database cross references - CRNKL1
ExoCarta ExoCarta_51340
Vesiclepedia VP_51340
Entrez Gene 51340
HGNC 15762
MIM 610952
UniProt Q9BZJ0  
 CRNKL1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for CRNKL1
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IDA
    protein binding GO:0005515 IPI
Biological Process
    spliceosomal complex assembly GO:0000245 IBA
    spliceosomal complex assembly GO:0000245 IDA
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IC
    mRNA splicing, via spliceosome GO:0000398 IDA
Subcellular Localization
    Prp19 complex GO:0000974 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    spliceosomal complex GO:0005681 IDA
    nuclear speck GO:0016607 IEA
    U2-type catalytic step 2 spliceosome GO:0071007 IBA
    U2-type catalytic step 2 spliceosome GO:0071007 IDA
    catalytic step 2 spliceosome GO:0071013 IDA
    post-mRNA release spliceosomal complex GO:0071014 IBA
 Experiment description of studies that identified CRNKL1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CRNKL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNRPB2 6629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 NUPL2 11097
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 RALYL 138046
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HNRNPC 3183
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
6 SNRPG 6637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ANK2 287
Affinity Capture-MS Homo sapiens
8 SNRPE 6635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 COIL  
Proximity Label-MS Homo sapiens
10 FBXO7 25793
Affinity Capture-MS Homo sapiens
11 PPIE 10450
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
12 ACTC1 70
Proximity Label-MS Homo sapiens
13 CTNNBL1 56259
Affinity Capture-MS Homo sapiens
14 DHX8 1659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
15 Ccdc12  
Affinity Capture-MS Mus musculus
16 HNRNPCL1 343069
Affinity Capture-MS Homo sapiens
17 DVL3 1857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 AAR2 25980
Affinity Capture-MS Homo sapiens
19 CCDC94  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 SYF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SNRPF 6636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SNRPD2 6633
Co-fractionation Homo sapiens
23 ILF2 3608
Affinity Capture-MS Homo sapiens
24 HNRNPUL2 221092
Affinity Capture-MS Homo sapiens
25 USB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 AQR 9716
Co-fractionation Homo sapiens
27 CAPZB 832
Affinity Capture-MS Homo sapiens
28 SNRNP70 6625
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
29 RNPS1 10921
Affinity Capture-MS Homo sapiens
30 Snw1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
31 ISY1 57461
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
32 EIF4A3 9775
Affinity Capture-MS Homo sapiens
33 WDR83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 Isy1 57905
Affinity Capture-MS Mus musculus
35 NPM1 4869
Affinity Capture-MS Homo sapiens
36 SNRPC 6631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 OBSL1 23363
Affinity Capture-MS Homo sapiens
38 EED  
Affinity Capture-MS Homo sapiens
39 Prpf8 192159
Affinity Capture-MS Mus musculus
40 PRCC  
Affinity Capture-MS Homo sapiens
41 MECP2 4204
Affinity Capture-MS Homo sapiens
42 MYCN  
Affinity Capture-MS Homo sapiens
43 NCSTN 23385
Affinity Capture-MS Homo sapiens
44 LIN28A  
Affinity Capture-MS Homo sapiens
45 Bcas2  
Affinity Capture-MS Mus musculus
46 MIB1 57534
Proximity Label-MS Homo sapiens
47 PRPF8 10594
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
48 DDX21 9188
Affinity Capture-MS Homo sapiens
49 PARK2  
Affinity Capture-MS Homo sapiens
50 PPIL1 51645
Affinity Capture-MS Homo sapiens
51 SUPT5H 6829
Affinity Capture-MS Homo sapiens
52 SRSF3 6428
Affinity Capture-MS Homo sapiens
53 PNN 5411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 PSMD14 10213
Affinity Capture-MS Homo sapiens
55 SRSF6 6431
Affinity Capture-MS Homo sapiens
56 SF3B1 23451
Affinity Capture-MS Homo sapiens
57 SNRNP200 23020
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
58 RPL5 6125
Affinity Capture-MS Homo sapiens
59 NTRK1 4914
Affinity Capture-MS Homo sapiens
60 U2AF2 11338
Co-fractionation Homo sapiens
61 KIAA1143  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 SNRPN 6638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 MAGOH 4116
Affinity Capture-MS Homo sapiens
64 CCDC12  
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
65 ELAVL2 1993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 RBM39 9584
Affinity Capture-MS Homo sapiens
67 PRPF4B 8899
Affinity Capture-MS Homo sapiens
68 C11orf57  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SLC2A4  
Affinity Capture-MS Homo sapiens
70 LMNB1 4001
Affinity Capture-MS Homo sapiens
71 ZCCHC10  
Affinity Capture-MS Homo sapiens
72 SIRT7  
Affinity Capture-MS Homo sapiens
73 SNRPB 6628
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 PLRG1 5356
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 CD6 923
Affinity Capture-MS Homo sapiens
76 XAB2 56949
Co-fractionation Homo sapiens
77 DNAJC17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 HNRNPA1 3178
Co-fractionation Homo sapiens
79 TSSC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 ZC3H11A  
Affinity Capture-MS Homo sapiens
81 SUZ12  
Affinity Capture-MS Homo sapiens
82 SART1 9092
Co-fractionation Homo sapiens
83 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
84 SF3A1 10291
Affinity Capture-MS Homo sapiens
85 EFTUD2 9343
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
86 LZTS2 84445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 PRPF19 27339
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 PSPC1 55269
Affinity Capture-MS Homo sapiens
89 RPA3 6119
Proximity Label-MS Homo sapiens
90 ZC3H18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 DLST 1743
Affinity Capture-MS Homo sapiens
92 TOP1 7150
Affinity Capture-MS Homo sapiens
93 RNF2  
Affinity Capture-MS Homo sapiens
94 BMI1  
Affinity Capture-MS Homo sapiens
95 ISY1-RAB43 100534599
Co-fractionation Homo sapiens
96 RPS16 6217
Affinity Capture-MS Homo sapiens
97 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
98 EAPP  
Affinity Capture-MS Homo sapiens
99 BCAS2 10286
Affinity Capture-MS Homo sapiens
100 CPSF6 11052
Affinity Capture-MS Homo sapiens
101 Cdc5l 71702
Affinity Capture-MS Mus musculus
102 CDC40  
Co-fractionation Homo sapiens
103 SSRP1 6749
Affinity Capture-MS Homo sapiens
104 MEN1 4221
Affinity Capture-MS Homo sapiens
105 HNRNPA2B1 3181
Co-fractionation Homo sapiens
106 SNRPA 6626
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
107 CCDC130  
Affinity Capture-MS Homo sapiens
108 BID  
Two-hybrid Homo sapiens
109 HSPB1 3315
Two-hybrid Homo sapiens
110 CHD4 1108
Affinity Capture-MS Homo sapiens
111 PUF60 22827
Affinity Capture-MS Homo sapiens
112 SNRNP40 9410
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
113 SNIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 SRSF7 6432
Affinity Capture-MS Homo sapiens
115 PINK1  
Affinity Capture-MS Homo sapiens
116 CIT 11113
Affinity Capture-MS Homo sapiens
117 SNW1 22938
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
118 KRAS 3845
Negative Genetic Homo sapiens
119 SMARCD3  
Co-fractionation Homo sapiens
120 SNRPA1 6627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 PRMT1 3276
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here