Gene description for Isy1
Gene name ISY1 splicing factor homolog (S. cerevisiae)
Gene symbol Isy1
Other names/aliases 5830446M03Rik
AI181014
AU020769
Species Mus musculus
 Database cross references - Isy1
ExoCarta ExoCarta_57905
Vesiclepedia VP_57905
Entrez Gene 57905
UniProt Q69ZQ2  
 Isy1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Isy1
Biological Process
    generation of catalytic spliceosome for second transesterification step GO:0000350 IBA
    generation of catalytic spliceosome for second transesterification step GO:0000350 IEA
    mRNA 3'-splice site recognition GO:0000389 IBA
    mRNA splicing, via spliceosome GO:0000398 ISS
Subcellular Localization
    Prp19 complex GO:0000974 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    U2-type catalytic step 1 spliceosome GO:0071006 ISO
    U2-type catalytic step 1 spliceosome GO:0071006 ISS
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
    post-mRNA release spliceosomal complex GO:0071014 IBA
    post-spliceosomal complex GO:0071020 IBA
 Experiment description of studies that identified Isy1 in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Isy1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DHX34 9704
Affinity Capture-MS Homo sapiens
2 CCDC12  
Affinity Capture-MS Homo sapiens
3 CACTIN  
Affinity Capture-MS Homo sapiens
4 ATP5L 10632
Affinity Capture-MS Homo sapiens
5 SDE2  
Affinity Capture-MS Homo sapiens
6 KEAP1 9817
Affinity Capture-MS Homo sapiens
7 DHX8 1659
Affinity Capture-MS Homo sapiens
8 PFDN6 10471
Affinity Capture-MS Homo sapiens
9 C1orf52  
Affinity Capture-MS Homo sapiens
10 CRNKL1 51340
Affinity Capture-MS Homo sapiens
11 SLU7  
Affinity Capture-MS Homo sapiens
12 Atg16l1  
Affinity Capture-MS Mus musculus
13 CHD9 80205
Affinity Capture-MS Homo sapiens
14 AQR 9716
Affinity Capture-MS Homo sapiens
15 ZNF830  
Affinity Capture-MS Homo sapiens
16 XAB2 56949
Affinity Capture-MS Homo sapiens
17 LACTB 114294
Affinity Capture-MS Homo sapiens
18 PPIE 10450
Affinity Capture-MS Homo sapiens
19 CDC40  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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