Gene description for CHD9
Gene name chromodomain helicase DNA binding protein 9
Gene symbol CHD9
Other names/aliases AD013
CHD-9
CReMM
KISH2
PRIC320
Species Homo sapiens
 Database cross references - CHD9
ExoCarta ExoCarta_80205
Vesiclepedia VP_80205
Entrez Gene 80205
HGNC 25701
UniProt Q3L8U1  
 CHD9 identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
 Gene ontology annotations for CHD9
Molecular Function
    DNA binding GO:0003677 IEA
    DNA clamp loader activity GO:0003689 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 IEA
    double-stranded DNA helicase activity GO:0036121 IEA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    DNA duplex unwinding GO:0032508 IEA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IDA
 Experiment description of studies that identified CHD9 in exosomes
1
Experiment ID 275
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
2
Experiment ID 274
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for CHD9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 ITGB1 3688
Cross-Linking-MS (XL-MS) Homo sapiens
3 PRICKLE3  
Affinity Capture-MS Homo sapiens
4 MECP2 4204
Affinity Capture-MS Homo sapiens
5 FGF11  
Affinity Capture-MS Homo sapiens
6 Dclre1b  
Affinity Capture-MS Mus musculus
7 MAD2L1 4085
Affinity Capture-MS Homo sapiens
8 CAMKV 79012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 COIL  
Proximity Label-MS Homo sapiens
10 EEF1G 1937
Cross-Linking-MS (XL-MS) Homo sapiens
11 PRKCD 5580
Affinity Capture-MS Homo sapiens
12 PDAP1 11333
Cross-Linking-MS (XL-MS) Homo sapiens
13 MDK 4192
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 EIF1AX 1964
Cross-Linking-MS (XL-MS) Homo sapiens
15 EPB41L3 23136
Affinity Capture-MS Homo sapiens
16 FGF12  
Affinity Capture-MS Homo sapiens
17 FGG 2266
Co-fractionation Homo sapiens
18 EPB41L5 57669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 PLCD3 113026
Affinity Capture-MS Homo sapiens
20 EDEM1  
Affinity Capture-MS Homo sapiens
21 PPIE 10450
Affinity Capture-MS Homo sapiens
22 C1orf35  
Affinity Capture-MS Homo sapiens
23 ANKRD36B  
Affinity Capture-MS Homo sapiens
24 Crnkl1  
Affinity Capture-MS Mus musculus
25 Cep152  
Affinity Capture-MS Mus musculus
26 APEX1 328
Affinity Capture-RNA Homo sapiens
27 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 C11orf30  
Affinity Capture-MS Homo sapiens
29 MYC  
Affinity Capture-MS Homo sapiens
30 VPS26B 112936
Affinity Capture-MS Homo sapiens
31 Kif4  
Affinity Capture-MS Mus musculus
32 KIF23 9493
Affinity Capture-MS Homo sapiens
33 NFKBIL1  
Affinity Capture-MS Homo sapiens
34 Isy1 57905
Affinity Capture-MS Mus musculus
35 BRD4 23476
Affinity Capture-MS Homo sapiens
36 TULP3 7289
Affinity Capture-MS Homo sapiens
37 SULF2 55959
Affinity Capture-MS Homo sapiens
38 HNRNPU 3192
Cross-Linking-MS (XL-MS) Homo sapiens
39 Cdca5  
Affinity Capture-MS Mus musculus
40 ADAMTS1 9510
Affinity Capture-MS Homo sapiens
41 PHF12  
Affinity Capture-MS Homo sapiens
42 SYT2  
Affinity Capture-MS Homo sapiens
43 2510003E04Rik  
Affinity Capture-MS Mus musculus
44 Wipi2 74781
Affinity Capture-MS Mus musculus
45 BRD3 8019
Affinity Capture-MS Homo sapiens
46 CEBPA  
Proximity Label-MS Homo sapiens
47 DAXX  
Affinity Capture-MS Homo sapiens
48 C10orf2 56652
Affinity Capture-MS Homo sapiens
49 CT45A5  
Affinity Capture-MS Homo sapiens
50 NKAP 79576
Affinity Capture-MS Homo sapiens
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