Gene description for SULF2
Gene name sulfatase 2
Gene symbol SULF2
Other names/aliases HSULF-2
Species Homo sapiens
 Database cross references - SULF2
ExoCarta ExoCarta_55959
Vesiclepedia VP_55959
Entrez Gene 55959
HGNC 20392
MIM 610013
UniProt Q8IWU5  
 SULF2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Keratinocytes 19530224    
Keratinocytes 19530224    
 Gene ontology annotations for SULF2
Molecular Function
    arylsulfatase activity GO:0004065 IDA
    arylsulfatase activity GO:0004065 IMP
    calcium ion binding GO:0005509 IEA
    glycosaminoglycan binding GO:0005539 IBA
    N-acetylglucosamine-6-sulfatase activity GO:0008449 IBA
    N-acetylglucosamine-6-sulfatase activity GO:0008449 IDA
    N-acetylglucosamine-6-sulfatase activity GO:0008449 IMP
Biological Process
    kidney development GO:0001822 ISS
    chondrocyte development GO:0002063 ISS
    glomerular filtration GO:0003094 ISS
    response to wounding GO:0009611 IEA
    positive regulation of vascular endothelial growth factor production GO:0010575 IBA
    positive regulation of vascular endothelial growth factor production GO:0010575 ISS
    esophagus smooth muscle contraction GO:0014846 ISS
    positive regulation of Wnt signaling pathway GO:0030177 IBA
    positive regulation of Wnt signaling pathway GO:0030177 IDA
    heparan sulfate proteoglycan metabolic process GO:0030201 IBA
    heparan sulfate proteoglycan metabolic process GO:0030201 IDA
    heparan sulfate proteoglycan metabolic process GO:0030201 NAS
    glomerular basement membrane development GO:0032836 IBA
    glomerular basement membrane development GO:0032836 ISS
    glial cell-derived neurotrophic factor receptor signaling pathway GO:0035860 ISS
    negative regulation of fibroblast growth factor receptor signaling pathway GO:0040037 IBA
    negative regulation of fibroblast growth factor receptor signaling pathway GO:0040037 ISS
    embryonic skeletal system development GO:0048706 ISS
    cartilage development GO:0051216 ISS
    bone development GO:0060348 ISS
    innervation GO:0060384 ISS
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
    liver regeneration GO:0097421 IEA
    positive regulation of endothelin production GO:1904472 IEA
    regulation of hepatocyte proliferation GO:2000345 IEA
Subcellular Localization
    extracellular region GO:0005576 IMP
    extracellular space GO:0005615 NAS
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    Golgi stack GO:0005795 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISS
    cell surface GO:0009986 IBA
    cell surface GO:0009986 IDA
 Experiment description of studies that identified SULF2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 189
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 190
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SULF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CENPB 1059
Affinity Capture-MS Homo sapiens
2 CCL3  
Affinity Capture-MS Homo sapiens
3 ZC3H18  
Affinity Capture-MS Homo sapiens
4 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CEACAM8 1088
Affinity Capture-MS Homo sapiens
6 LCN6  
Affinity Capture-MS Homo sapiens
7 NOS1AP  
Affinity Capture-MS Homo sapiens
8 TNRC18  
Affinity Capture-MS Homo sapiens
9 AKAP11 11215
Affinity Capture-MS Homo sapiens
10 APC  
Affinity Capture-MS Homo sapiens
11 NFKBIL1  
Affinity Capture-MS Homo sapiens
12 BRD2  
Affinity Capture-MS Homo sapiens
13 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
14 APP 351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 MRPS18C  
Affinity Capture-MS Homo sapiens
16 NEK7 140609
Affinity Capture-MS Homo sapiens
17 DDA1  
Affinity Capture-MS Homo sapiens
18 UBE2O 63893
Affinity Capture-MS Homo sapiens
19 DEFA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 GNS 2799
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 KLHDC10 23008
Affinity Capture-MS Homo sapiens
22 MRPS5 64969
Affinity Capture-MS Homo sapiens
23 SRRM2 23524
Affinity Capture-MS Homo sapiens
24 BAGE2  
Affinity Capture-MS Homo sapiens
25 BOD1L1 259282
Affinity Capture-MS Homo sapiens
26 BRD4 23476
Affinity Capture-MS Homo sapiens
27 MRPS7 51081
Affinity Capture-MS Homo sapiens
28 MECP2 4204
Affinity Capture-MS Homo sapiens
29 SETD1A 9739
Affinity Capture-MS Homo sapiens
30 SULF1 23213
Affinity Capture-MS Homo sapiens
31 GPR156  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 NOTCH2 4853
Affinity Capture-MS Homo sapiens
33 CXXC1  
Affinity Capture-MS Homo sapiens
34 ACIN1 22985
Affinity Capture-MS Homo sapiens
35 CCDC85C  
Affinity Capture-MS Homo sapiens
36 VPRBP 9730
Affinity Capture-MS Homo sapiens
37 C1orf54  
Affinity Capture-MS Homo sapiens
38 PDP2  
Affinity Capture-MS Homo sapiens
39 PNMA2  
Affinity Capture-MS Homo sapiens
40 UBTF 7343
Affinity Capture-MS Homo sapiens
41 SUMF1 285362
Affinity Capture-MS Homo sapiens
42 CCDC22 28952
Affinity Capture-MS Homo sapiens
43 FBLN5 10516
Affinity Capture-MS Homo sapiens
44 SHCBP1 79801
Affinity Capture-MS Homo sapiens
45 SPANXN2  
Affinity Capture-MS Homo sapiens
46 IGFL3  
Affinity Capture-MS Homo sapiens
47 PRKAR1B  
Affinity Capture-MS Homo sapiens
48 LRP1B 53353
Affinity Capture-MS Homo sapiens
49 COMMD3 23412
Affinity Capture-MS Homo sapiens
50 C1QTNF7  
Affinity Capture-MS Homo sapiens
51 TXNDC11 51061
Affinity Capture-MS Homo sapiens
52 CHD9 80205
Affinity Capture-MS Homo sapiens
53 UBR2 23304
Affinity Capture-MS Homo sapiens
54 C7orf34  
Affinity Capture-MS Homo sapiens
55 DKK3 27122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 GPIHBP1  
Affinity Capture-MS Homo sapiens
57 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 LRP4 4038
Affinity Capture-MS Homo sapiens
59 PON2 5445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 PDGFRA 5156
Affinity Capture-MS Homo sapiens
61 PRG2  
Affinity Capture-MS Homo sapiens
62 COMMD2 51122
Affinity Capture-MS Homo sapiens
63 PPP1R15B  
Affinity Capture-MS Homo sapiens
64 SPON1 10418
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 LRRC4C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 FBXO6 26270
Affinity Capture-MS Homo sapiens
67 NAF1  
Affinity Capture-MS Homo sapiens
68 BRMS1L  
Affinity Capture-MS Homo sapiens
69 SDC2 6383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 INS 3630
Affinity Capture-MS Homo sapiens
71 UBR1 197131
Affinity Capture-MS Homo sapiens
72 RARRES1 5918
Affinity Capture-MS Homo sapiens
73 MYC  
Dosage Lethality Homo sapiens
74 FBXO2 26232
Affinity Capture-MS Homo sapiens
75 SCGB2A2  
Affinity Capture-MS Homo sapiens
76 CCDC85B  
Affinity Capture-MS Homo sapiens
77 ANKS3  
Affinity Capture-MS Homo sapiens
78 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 EDDM3B  
Affinity Capture-MS Homo sapiens
80 TSPYL4 23270
Affinity Capture-MS Homo sapiens
81 SIN3B  
Affinity Capture-MS Homo sapiens
82 BACH2 60468
Affinity Capture-MS Homo sapiens
83 KLHL15  
Affinity Capture-MS Homo sapiens
84 CCL3L1  
Affinity Capture-MS Homo sapiens
85 NAAA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 EPB41L5 57669
Affinity Capture-MS Homo sapiens
87 SIN3A  
Affinity Capture-MS Homo sapiens
88 HECTD3 79654
Affinity Capture-MS Homo sapiens
89 ZZEF1 23140
Affinity Capture-MS Homo sapiens
90 CDH23 64072
Affinity Capture-MS Homo sapiens
91 OS9 10956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 EDEM2  
Affinity Capture-MS Homo sapiens
93 PRKACB 5567
Affinity Capture-MS Homo sapiens
94 ZCCHC10  
Affinity Capture-MS Homo sapiens
95 LRP2 4036
Affinity Capture-MS Homo sapiens
96 SDC4 6385
Affinity Capture-MS Homo sapiens
97 BRD1 23774
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 MLLT3 4300
Affinity Capture-MS Homo sapiens
99 G2E3  
Affinity Capture-MS Homo sapiens
100 CWC22 57703
Affinity Capture-MS Homo sapiens
101 SPEN 23013
Affinity Capture-MS Homo sapiens
102 PTCH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 IL5RA  
Affinity Capture-MS Homo sapiens
104 DCAF5  
Affinity Capture-MS Homo sapiens
105 MRPS9 64965
Affinity Capture-MS Homo sapiens
106 TRPC4AP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 CASQ2 845
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SULF2 is involved
No pathways found





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