Gene description for INS
Gene name insulin
Gene symbol INS
Other names/aliases IDDM
Species Homo sapiens
 Database cross references - INS
ExoCarta ExoCarta_3630
Entrez Gene 3630
HGNC 6081
MIM 176730
UniProt P01308  
 INS identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for INS
Molecular Function
    protein binding GO:0005515 IPI
    insulin-like growth factor receptor binding GO:0005159 IPI
    hormone activity GO:0005179 NAS
    identical protein binding GO:0042802 IPI
    protease binding GO:0002020 IPI
    insulin receptor binding GO:0005158 IPI
Biological Process
    negative regulation of NAD(P)H oxidase activity GO:0033861 IDA
    positive regulation of glycogen biosynthetic process GO:0045725 IDA
    acute-phase response GO:0006953 IDA
    regulation of transmembrane transporter activity GO:0022898 IDA
    small molecule metabolic process GO:0044281 TAS
    wound healing GO:0042060 IDA
    regulation of protein localization GO:0032880 IDA
    positive regulation of MAPK cascade GO:0043410 IDA
    insulin receptor signaling pathway GO:0008286 TAS
    negative regulation of feeding behavior GO:2000252 IDA
    alpha-beta T cell activation GO:0046631 IDA
    positive regulation of protein localization to nucleus GO:1900182 IDA
    positive regulation of vasodilation GO:0045909 NAS
    negative regulation of glycogen catabolic process GO:0045818 IMP
    positive regulation of protein autophosphorylation GO:0031954 ISS
    positive regulation of brown fat cell differentiation GO:0090336 TAS
    glucose homeostasis GO:0042593 IMP
    cell-cell signaling GO:0007267 IC
    negative regulation of respiratory burst involved in inflammatory response GO:0060266 IDA
    positive regulation of insulin receptor signaling pathway GO:0046628 IDA
    negative regulation of protein secretion GO:0050709 IDA
    positive regulation of mitotic nuclear division GO:0045840 IDA
    negative regulation of lipid catabolic process GO:0050995 NAS
    activation of protein kinase B activity GO:0032148 IDA
    positive regulation of peptide hormone secretion GO:0090277 TAS
    fatty acid homeostasis GO:0055089 IMP
    negative regulation of gluconeogenesis GO:0045721 NAS
    positive regulation of cell growth GO:0030307 NAS
    positive regulation of cell proliferation GO:0008284 IDA
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 IDA
    negative regulation of proteolysis GO:0045861 IMP
    negative regulation of protein catabolic process GO:0042177 IDA
    positive regulation of lipid biosynthetic process GO:0046889 NAS
    positive regulation of glucose import GO:0046326 IDA
    positive regulation of nitric oxide biosynthetic process GO:0045429 NAS
    negative regulation of vasodilation GO:0045908 NAS
    positive regulation of nitric-oxide synthase activity GO:0051000 NAS
    energy reserve metabolic process GO:0006112 TAS
    positive regulation of cell differentiation GO:0045597 NAS
    MAPK cascade GO:0000165 IDA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IDA
    negative regulation of acute inflammatory response GO:0002674 IDA
    endocrine pancreas development GO:0031018 TAS
    positive regulation of DNA replication GO:0045740 IDA
    regulation of insulin secretion GO:0050796 TAS
    negative regulation of protein oligomerization GO:0032460 IDA
    negative regulation of fatty acid metabolic process GO:0045922 IMP
    regulation of protein secretion GO:0050708 IDA
    positive regulation of cellular protein metabolic process GO:0032270 IMP
    positive regulation of cell migration GO:0030335 ISS
    regulation of transcription, DNA-templated GO:0006355 NAS
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 NAS
    glucose metabolic process GO:0006006 IEA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    cellular protein metabolic process GO:0044267 TAS
    G-protein coupled receptor signaling pathway GO:0007186 IDA
    positive regulation of protein kinase B signaling GO:0051897 IDA
    positive regulation of cytokine secretion GO:0050715 IDA
    positive regulation of respiratory burst GO:0060267 IDA
    positive regulation of glycolytic process GO:0045821 IMP
    glucose transport GO:0015758 IDA
    regulation of cellular amino acid metabolic process GO:0006521 IMP
Subcellular Localization
    Golgi lumen GO:0005796 TAS
    extracellular region GO:0005576 TAS
    endosome lumen GO:0031904 TAS
    extracellular space GO:0005615 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    secretory granule lumen GO:0034774 TAS
 Experiment description of studies that identified INS in exosomes
Experiment ID 235
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for INS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 INS 3630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 IGFBP7 3490
Invitro Homo sapiens
3 SYTL4 94121
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 MLL  
Biochemical Activity Homo sapiens
5 IGF1R 3480
Invivo Homo sapiens
6 TXNDC17 84817
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
7 HLA-DQB1  
Affinity Capture-MS Homo sapiens
8 LRP2 4036
Invitro Homo sapiens
9 NOV  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
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