Gene description for UBR1
Gene name ubiquitin protein ligase E3 component n-recognin 1
Gene symbol UBR1
Other names/aliases JBS
Species Homo sapiens
 Database cross references - UBR1
ExoCarta ExoCarta_197131
Vesiclepedia VP_197131
Entrez Gene 197131
HGNC 16808
MIM 605981
UniProt Q8IWV7  
 UBR1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for UBR1
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
    L-leucine binding GO:0070728 IDA
Biological Process
    protein ubiquitination GO:0016567 IBA
    protein ubiquitination GO:0016567 IEA
    negative regulation of TOR signaling GO:0032007 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    cellular response to L-leucine GO:0071233 IDA
    ubiquitin-dependent protein catabolic process via the N-end rule pathway GO:0071596 IBA
    ubiquitin-dependent protein catabolic process via the N-end rule pathway GO:0071596 IDA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IBA
    proteasome complex GO:0000502 IEA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified UBR1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for UBR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLDIP3 84271
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CRH  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 UBE2A  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 SCML4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HOXA7  
Affinity Capture-MS Homo sapiens
6 ANXA3 306
Two-hybrid Homo sapiens
7 GADD45A  
Two-hybrid Homo sapiens
8 USP5 8078
Co-fractionation Homo sapiens
9 ARL15 54622
Two-hybrid Homo sapiens
10 CFC1  
Affinity Capture-MS Homo sapiens
11 GFAP 2670
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CRHBP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 CDC6  
Reconstituted Complex Homo sapiens
14 TAC3  
Two-hybrid Homo sapiens
15 LYPD6B 130576
Affinity Capture-MS Homo sapiens
16 UBC 7316
Affinity Capture-MS Homo sapiens
17 CSE1L 1434
Co-fractionation Homo sapiens
18 CPQ 10404
Affinity Capture-MS Homo sapiens
19 RASD1  
Affinity Capture-MS Homo sapiens
20 EGFLAM  
Affinity Capture-MS Homo sapiens
21 MYEOV2 150678
Affinity Capture-MS Homo sapiens
22 AMHR2  
Affinity Capture-MS Homo sapiens
23 ARL3 403
Two-hybrid Homo sapiens
24 BTLA  
Affinity Capture-MS Homo sapiens
25 SULF2 55959
Affinity Capture-MS Homo sapiens
26 MTG2  
Affinity Capture-MS Homo sapiens
27 TGFB1 7040
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 VARS 7407
Co-fractionation Homo sapiens
29 NUDT3 11165
Two-hybrid Homo sapiens
30 RECQL4  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
31 FTSJ2 29960
Affinity Capture-MS Homo sapiens
32 KRT31 3881
Affinity Capture-MS Homo sapiens
33 LAMTOR3 8649
Two-hybrid Homo sapiens
34 CROCC 9696
Affinity Capture-MS Homo sapiens
35 IGF2 3481
Affinity Capture-MS Homo sapiens
36 FAF1 11124
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
37 CCDC130  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 NSFL1C 55968
Affinity Capture-Western Homo sapiens
39 FAF2 23197
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
40 FEM1A  
Affinity Capture-MS Homo sapiens
41 GSK3B 2932
Two-hybrid Homo sapiens
42 SERPINB9 5272
Two-hybrid Homo sapiens
43 RGS4  
Protein-peptide Homo sapiens
44 RABGEF1 27342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 Mgp 17313
Affinity Capture-MS Mus musculus
46 SPATA24  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 LIPT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CTSO  
Affinity Capture-MS Homo sapiens
49 IBSP  
Affinity Capture-MS Homo sapiens
50 BAGE2  
Affinity Capture-MS Homo sapiens
51 CLCC1 23155
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 SPAG11A  
Affinity Capture-MS Homo sapiens
53 SIPA1L2  
Affinity Capture-MS Homo sapiens
54 NTRK1 4914
Affinity Capture-MS Homo sapiens
55 NXPH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 BMP7 655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 APBA1  
Affinity Capture-MS Homo sapiens
58 WNT10A  
Affinity Capture-MS Homo sapiens
59 VCL 7414
Co-fractionation Homo sapiens
60 CUL2 8453
Affinity Capture-MS Homo sapiens
61 UBE2B 7320
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
62 CBX7  
Affinity Capture-MS Homo sapiens
63 DNAJB11 51726
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
64 TIRAP  
Affinity Capture-MS Homo sapiens
65 CHRDL1 91851
Affinity Capture-MS Homo sapiens
66 ITM2B 9445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 C19orf66  
Affinity Capture-MS Homo sapiens
68 DLEU1  
Two-hybrid Homo sapiens
69 MRM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
71 KIAA1191  
Affinity Capture-MS Homo sapiens
72 SPSB4  
Affinity Capture-MS Homo sapiens
73 KRT33B 3884
Affinity Capture-MS Homo sapiens
74 XPO1 7514
Co-fractionation Homo sapiens
75 KRT86  
Affinity Capture-MS Homo sapiens
76 IL25  
Affinity Capture-MS Homo sapiens
77 UBXN7 26043
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
78 NDUFA4L2 56901
Two-hybrid Homo sapiens
79 UBE2Z 65264
Affinity Capture-Western Homo sapiens
80 LDLRAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 PF4V1 5197
Affinity Capture-MS Homo sapiens
82 GADD45GIP1  
Affinity Capture-MS Homo sapiens
83 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
84 FTH1 2495
Two-hybrid Homo sapiens
85 INSL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 INS 3630
Affinity Capture-MS Homo sapiens
87 DMTN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 NXF1 10482
Affinity Capture-RNA Homo sapiens
89 CDC42 998
Two-hybrid Homo sapiens
90 UBE2D2 7322
Reconstituted Complex Homo sapiens
91 FOS 2353
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
92 RPUSD4 84881
Affinity Capture-MS Homo sapiens
93 CNR2  
Affinity Capture-MS Homo sapiens
94 ARL4D  
Two-hybrid Homo sapiens
95 EPN3 55040
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 GNLY  
Affinity Capture-MS Homo sapiens
97 CD244 51744
Affinity Capture-MS Homo sapiens
98 PPP1R32  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 LAMA4 3910
Two-hybrid Homo sapiens
100 TP53I3  
Two-hybrid Homo sapiens
101 POGLUT1 56983
Affinity Capture-MS Homo sapiens
102 NPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 PDGFD 80310
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 TDGF1  
Affinity Capture-MS Homo sapiens
105 UBXN1 51035
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
106 MRPL18 29074
Affinity Capture-MS Homo sapiens
107 UBE2D1 7321
Co-purification Homo sapiens
108 PFAS 5198
Co-fractionation Homo sapiens
109 COX7A1  
Affinity Capture-MS Homo sapiens
110 ZRANB1 54764
Affinity Capture-MS Homo sapiens
111 FGL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 NES 10763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 NRP1 8829
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 SPCS3 60559
Affinity Capture-MS Homo sapiens
115 PSG1  
Affinity Capture-MS Homo sapiens
116 SPRY2 10253
Two-hybrid Homo sapiens
117 ZNF593  
Co-fractionation Homo sapiens
118 ATP6V1A 523
Co-fractionation Homo sapiens
119 SPINK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 CLEC2D  
Affinity Capture-MS Homo sapiens
121 EDEM1  
Affinity Capture-MS Homo sapiens
122 AGMAT  
Affinity Capture-MS Homo sapiens
123 PNLIPRP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
124 SIL1 64374
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 SHFM1 7979
Affinity Capture-MS Homo sapiens
126 LY6G5C  
Affinity Capture-MS Homo sapiens
127 UCHL3 7347
Co-fractionation Homo sapiens
128 DNPH1 10591
Co-fractionation Homo sapiens
129 ZNF383  
Affinity Capture-MS Homo sapiens
130 BTBD2  
Two-hybrid Homo sapiens
131 ADAMTS12 81792
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 BCL2L1 598
Two-hybrid Homo sapiens
133 PTPRS 5802
Two-hybrid Homo sapiens
134 CROCCP2  
Affinity Capture-MS Homo sapiens
135 VIM 7431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 PLIN2 123
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
137 PRSS23 11098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 GREM2 64388
Affinity Capture-MS Homo sapiens
139 PROSER2 254427
Affinity Capture-MS Homo sapiens
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