Gene description for NRP1
Gene name neuropilin 1
Gene symbol NRP1
Other names/aliases BDCA4
CD304
NP1
NRP
VEGF165R
Species Homo sapiens
 Database cross references - NRP1
ExoCarta ExoCarta_8829
Entrez Gene 8829
HGNC 8004
MIM 602069
UniProt O14786  
 NRP1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocellular carcinoma cells 26054723    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Tabecular meshwork cells 21362503    
 Gene ontology annotations for NRP1
Molecular Function
    vascular endothelial growth factor-activated receptor activity GO:0005021 NAS
    cytokine binding GO:0019955 NAS
    vascular endothelial growth factor binding GO:0038085 IPI
    growth factor binding GO:0019838 TAS
    heparin binding GO:0008201 IEA
    metal ion binding GO:0046872 IEA
    semaphorin receptor activity GO:0017154 NAS
    protein binding GO:0005515 IPI
    coreceptor activity GO:0015026 TAS
Biological Process
    angiogenesis GO:0001525 ISS
    coronary artery morphogenesis GO:0060982 IEA
    axonogenesis involved in innervation GO:0060385 ISS
    patterning of blood vessels GO:0001569 ISS
    vestibulocochlear nerve structural organization GO:0021649 IEA
    facioacoustic ganglion development GO:1903375 IEA
    platelet-derived growth factor receptor signaling pathway GO:0048008 IMP
    semaphorin-plexin signaling pathway involved in neuron projection guidance GO:1902285 ISS
    renal artery morphogenesis GO:0061441 IEA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    cellular response to hepatocyte growth factor stimulus GO:0035729 IMP
    facial nerve structural organization GO:0021612 IEA
    sympathetic ganglion development GO:0061549 ISS
    regulation of vesicle-mediated transport GO:0060627 TAS
    positive regulation of axon extension involved in axon guidance GO:0048842 ISS
    nerve development GO:0021675 ISS
    toxin transport GO:1901998 IEA
    commissural neuron axon guidance GO:0071679 ISS
    organ morphogenesis GO:0009887 TAS
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISS
    axonal fasciculation GO:0007413 IEA
    response to wounding GO:0009611 IEA
    regulation of retinal ganglion cell axon guidance GO:0090259 ISS
    gonadotrophin-releasing hormone neuronal migration to the hypothalamus GO:0021828 IEA
    positive chemotaxis GO:0050918 ISS
    ganglion morphogenesis GO:0061552 IEA
    endothelial cell chemotaxis GO:0035767 IMP
    endothelial tip cell fate specification GO:0097102 ISS
    positive regulation of retinal ganglion cell axon guidance GO:1902336 ISS
    otic placode formation GO:0043049 IEA
    cell-cell signaling GO:0007267 TAS
    cell migration involved in sprouting angiogenesis GO:0002042 ISS
    sprouting angiogenesis GO:0002040 ISS
    positive regulation of endothelial cell migration GO:0010595 TAS
    ventral trunk neural crest cell migration GO:0036486 IEA
    branchiomotor neuron axon guidance GO:0021785 IEA
    protein localization to early endosome GO:1902946 ISS
    hepatocyte growth factor receptor signaling pathway GO:0048012 IMP
    VEGF-activated neuropilin signaling pathway GO:0038190 ISS
    neuron migration GO:0001764 ISS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    positive regulation of cytokine activity GO:0060301 TAS
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IEA
    trigeminal ganglion development GO:0061551 IEA
    artery morphogenesis GO:0048844 ISS
    trigeminal nerve structural organization GO:0021637 IEA
    sympathetic neuron projection extension GO:0097490 ISS
    semaphorin-plexin signaling pathway GO:0071526 NAS
    signal transduction GO:0007165 TAS
    semaphorin-plexin signaling pathway involved in axon guidance GO:1902287 IEA
    positive regulation of smooth muscle cell migration GO:0014911 TAS
    axon guidance GO:0007411 TAS
    angiogenesis involved in coronary vascular morphogenesis GO:0060978 ISS
    axon extension involved in axon guidance GO:0048846 ISS
    VEGF-activated neuropilin signaling pathway involved in axon guidance GO:1902378 IEA
    dendrite development GO:0016358 IEA
    neural crest cell migration involved in autonomic nervous system development GO:1901166 ISS
    negative regulation of axon extension involved in axon guidance GO:0048843 IEA
    positive regulation of endothelial cell proliferation GO:0001938 TAS
    retina vasculature morphogenesis in camera-type eye GO:0061299 ISS
    sympathetic neuron projection guidance GO:0097491 ISS
    dichotomous subdivision of terminal units involved in salivary gland branching GO:0060666 IEA
    sensory neuron axon guidance GO:0097374 IEA
    retinal ganglion cell axon guidance GO:0031290 ISS
    cellular response to vascular endothelial growth factor stimulus GO:0035924 ISS
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IMP
Subcellular Localization
    growth cone GO:0030426 IEA
    integral component of membrane GO:0016021 IEA
    plasma membrane GO:0005886 TAS
    extracellular space GO:0005615 IDA
    neuronal cell body GO:0043025 IEA
    semaphorin receptor complex GO:0002116 NAS
    focal adhesion GO:0005925 IDA
    sorting endosome GO:0097443 ISS
    cytoplasmic vesicle GO:0031410 TAS
    cytosol GO:0005829 IDA
    neurofilament GO:0005883 IEA
    cell surface GO:0009986 IEA
    early endosome GO:0005769 ISS
    axon GO:0030424 ISS
    receptor complex GO:0043235 TAS
 Experiment description of studies that identified NRP1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
5
Experiment ID 259
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 260
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 126
ISEV standards
EV Biophysical techniques
GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
8
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
9
Experiment ID 275
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
10
Experiment ID 274
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
11
Experiment ID 118
ISEV standards
EV Biophysical techniques
SDCBP|GAPDH
EV Cytosolic markers
CD9|CD81|FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21362503    
Organism Homo sapiens
Experiment description Protein profile of exosomes from trabecular meshwork cells.
Authors Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL.
Journal name JOP
Publication year 2011
Sample Tabecular meshwork cells
Sample name Tabecular meshwork cells
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.07-1.2 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for NRP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEMA3B  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
2 GIPC1 10755
Invivo Homo sapiens
Invitro Homo sapiens
3 VEGF  
Reconstituted Complex Homo sapiens
4 FIGF  
Invitro Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
5 PGF  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
6 SEMA3A  
Invivo Homo sapiens
7 VEGFB  
Invivo Homo sapiens
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