Gene description for PIP5K1A
Gene name phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
Gene symbol PIP5K1A
Other names/aliases -
Species Homo sapiens
 Database cross references - PIP5K1A
ExoCarta ExoCarta_8394
Entrez Gene 8394
HGNC 8994
MIM 603275
UniProt Q99755  
 PIP5K1A identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PIP5K1A
Molecular Function
    1-phosphatidylinositol-4-phosphate 5-kinase activity GO:0016308 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    kinase binding GO:0019900 IDA
Biological Process
    actin cytoskeleton reorganization GO:0031532 IMP
    activation of GTPase activity GO:0090630 IMP
    ruffle assembly GO:0097178 IMP
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    fibroblast migration GO:0010761 IEA
    cell migration GO:0016477 NAS
    phagocytosis GO:0006909 TAS
    phosphatidylinositol phosphorylation GO:0046854 TAS
    cell chemotaxis GO:0060326 IMP
    phospholipid metabolic process GO:0006644 TAS
    small molecule metabolic process GO:0044281 TAS
    protein targeting to plasma membrane GO:0072661 IMP
    keratinocyte differentiation GO:0030216 TAS
    glycerophospholipid metabolic process GO:0006650 TAS
    focal adhesion assembly GO:0048041 IMP
    phospholipid biosynthetic process GO:0008654 IDA
    signal transduction GO:0007165 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 IDA
    lamellipodium GO:0030027 IDA
    cytoplasm GO:0005737 IDA
    ruffle membrane GO:0032587 IDA
    plasma membrane GO:0005886 IDA
    focal adhesion GO:0005925 IDA
    mRNA cleavage and polyadenylation specificity factor complex GO:0005847 IDA
    nuclear speck GO:0016607 TAS
 Experiment description of studies that identified PIP5K1A in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PIP5K1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARF5 381
Affinity Capture-MS Homo sapiens
2 ARF6 382
Affinity Capture-MS Homo sapiens
3 PLD2 5338
Invivo Homo sapiens
Invitro Homo sapiens
4 PIP5KL1  
Affinity Capture-Western Homo sapiens
5 ARF1 375
Affinity Capture-MS Homo sapiens
6 PLD1 5337
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which PIP5K1A is involved
PathwayEvidenceSource
Synthesis of PIPs at the plasma membrane TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here