Gene description for HTATSF1
Gene name HIV-1 Tat specific factor 1
Gene symbol HTATSF1
Other names/aliases TAT-SF1
TATSF1
dJ196E23.2
Species Homo sapiens
 Database cross references - HTATSF1
ExoCarta ExoCarta_27336
Vesiclepedia VP_27336
Entrez Gene 27336
HGNC 5276
MIM 300346
UniProt O43719  
 HTATSF1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for HTATSF1
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    protein binding GO:0005515 IPI
    chromatin-protein adaptor activity GO:0140463 IDA
    poly-ADP-D-ribose modification-dependent protein binding GO:0160004 IDA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IDA
    mRNA splicing, via spliceosome GO:0000398 NAS
    double-strand break repair via homologous recombination GO:0000724 IDA
    chromatin organization GO:0006325 IEA
    U2-type prespliceosome assembly GO:1903241 IDA
    U2-type prespliceosome assembly GO:1903241 NAS
    protein localization to site of double-strand break GO:1990166 IDA
Subcellular Localization
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    spliceosomal complex GO:0005681 NAS
    U2-type spliceosomal complex GO:0005684 IBA
    U2-type spliceosomal complex GO:0005684 IDA
    U2 snRNP GO:0005686 IBA
    U2 snRNP GO:0005686 NAS
    site of double-strand break GO:0035861 IDA
 Experiment description of studies that identified HTATSF1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HTATSF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 SNRPG 6637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SNRPE 6635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 COIL  
Proximity Label-MS Homo sapiens
5 RIN3  
Affinity Capture-MS Homo sapiens
6 RNF25 64320
Affinity Capture-MS Homo sapiens
7 ACTC1 70
Proximity Label-MS Homo sapiens
8 ESF1 51575
Co-fractionation Homo sapiens
9 CSNK2A1 1457
Biochemical Activity Homo sapiens
10 APBB1  
Reconstituted Complex Homo sapiens
11 SNRPF 6636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SNRPD2 6633
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
13 SF3B2 10992
Affinity Capture-MS Homo sapiens
14 KIAA1429 25962
Affinity Capture-MS Homo sapiens
15 PGK1 5230
Co-fractionation Homo sapiens
16 GTF2F2 2963
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 NOLC1 9221
Co-fractionation Homo sapiens
18 SF3B1 23451
Affinity Capture-MS Homo sapiens
19 KATNAL2 83473
Co-fractionation Homo sapiens
20 ZNF512B  
Two-hybrid Homo sapiens
21 SF3A2 8175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
22 MED17  
Affinity Capture-MS Homo sapiens
23 TCEANC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SNX27 81609
Affinity Capture-MS Homo sapiens
25 MLLT1 4298
Co-fractionation Homo sapiens
26 SLU7  
Affinity Capture-MS Homo sapiens
27 TCOF1 6949
Co-fractionation Homo sapiens
28 SRP9 6726
Affinity Capture-MS Homo sapiens
29 WBP4  
Reconstituted Complex Homo sapiens
30 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
31 SUPT5H 6829
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
32 RBM7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CBX3 11335
Proximity Label-MS Homo sapiens
34 RPL5 6125
Co-fractionation Homo sapiens
35 SNRPB 6628
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 IWS1  
Co-fractionation Homo sapiens
37 PBXIP1 57326
Affinity Capture-MS Homo sapiens
38 NTRK1 4914
Affinity Capture-MS Homo sapiens
39 SNRPN 6638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SF3B6 51639
Affinity Capture-MS Homo sapiens
41 SRSF4 6429
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
42 PELP1 27043
Co-fractionation Homo sapiens
43 SEC61B 10952
Affinity Capture-MS Homo sapiens
44 CCNT1  
Reconstituted Complex Homo sapiens
45 SNRPB2 6629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 MAT2A 4144
Cross-Linking-MS (XL-MS) Homo sapiens
47 BCLAF1 9774
Co-fractionation Homo sapiens
48 SFPQ 6421
Co-fractionation Homo sapiens
49 FAM129B 64855
Co-fractionation Homo sapiens
50 ATL3 25923
Co-fractionation Homo sapiens
51 POP1 10940
Co-fractionation Homo sapiens
52 DNAJC2 27000
Affinity Capture-MS Homo sapiens
53 CD2BP2 10421
Affinity Capture-MS Homo sapiens
54 POLR2E 5434
Co-fractionation Homo sapiens
55 CUL3 8452
Affinity Capture-MS Homo sapiens
56 DNAJC17  
Affinity Capture-MS Homo sapiens
57 FLYWCH1  
Affinity Capture-MS Homo sapiens
58 NAF1  
Co-fractionation Homo sapiens
59 POLR2A 5430
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
60 RRP15  
Co-fractionation Homo sapiens
61 SF3A1 10291
Affinity Capture-MS Homo sapiens
62 AGO4  
Affinity Capture-MS Homo sapiens
63 RPA3 6119
Proximity Label-MS Homo sapiens
64 EGFR 1956
Affinity Capture-MS Homo sapiens
65 PHF5A 84844
Affinity Capture-MS Homo sapiens
66 Junb  
Affinity Capture-MS Mus musculus
67 FYTTD1  
Affinity Capture-MS Homo sapiens
68 PYHIN1  
Affinity Capture-MS Homo sapiens
69 PIP5K1A 8394
Affinity Capture-MS Homo sapiens
70 FEZ1  
Two-hybrid Homo sapiens
71 ARRB2 409
Affinity Capture-MS Homo sapiens
72 SRRM1 10250
Co-fractionation Homo sapiens
73 TP53 7157
Affinity Capture-MS Homo sapiens
74 SNRPA1 6627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 NGEF  
Affinity Capture-MS Homo sapiens
76 PTBP1 5725
Co-fractionation Homo sapiens
77 Sf3a1 67465
Affinity Capture-MS Mus musculus
78 DYNC1H1 1778
Co-fractionation Homo sapiens
79 NUP50 10762
Proximity Label-MS Homo sapiens
80 HDGFRP2 84717
Co-fractionation Homo sapiens
81 NCL 4691
Co-fractionation Homo sapiens
82 CDK9 1025
Co-fractionation Homo sapiens
83 PINK1  
Affinity Capture-MS Homo sapiens
84 METTL3  
Affinity Capture-MS Homo sapiens
85 STK11 6794
Affinity Capture-MS Homo sapiens
86 SERBP1 26135
Affinity Capture-MS Homo sapiens
87 PAF1 54623
Co-fractionation Homo sapiens
88 AP3D1 8943
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
89 NOTCH1 4851
Affinity Capture-MS Homo sapiens
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