Gene description for RNF25
Gene name ring finger protein 25
Gene symbol RNF25
Other names/aliases AO7
Species Homo sapiens
 Database cross references - RNF25
ExoCarta ExoCarta_64320
Entrez Gene 64320
HGNC 14662
MIM 616014
UniProt Q96BH1  
 RNF25 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RNF25
Molecular Function
    zinc ion binding GO:0008270 IEA
    ligase activity GO:0016874 IEA
    ubiquitin-protein transferase activity GO:0004842 ISS
    NF-kappaB binding GO:0051059 IPI
Biological Process
    protein ubiquitination GO:0016567 ISS
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified RNF25 in exosomes
1
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RNF25
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RELA  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which RNF25 is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome





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