Gene description for LCN2
Gene name lipocalin 2
Gene symbol LCN2
Other names/aliases 24p3
MSFI
NGAL
Species Homo sapiens
 Database cross references - LCN2
ExoCarta ExoCarta_3934
Vesiclepedia VP_3934
Entrez Gene 3934
HGNC 6526
MIM 600181
UniProt P80188  
 LCN2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
Urine 19056867    
 Gene ontology annotations for LCN2
Molecular Function
    iron ion binding GO:0005506 IDA
    iron ion binding GO:0005506 ISS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    iron ion sequestering activity GO:0140315 IDA
    enterobactin binding GO:1903981 IDA
Biological Process
    apoptotic process GO:0006915 IEA
    siderophore transport GO:0015891 ISS
    defense response to bacterium GO:0042742 IDA
    innate immune response GO:0045087 ISS
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
Subcellular Localization
    extracellular region GO:0005576 HDA
    extracellular region GO:0005576 ISS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    specific granule lumen GO:0035580 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified LCN2 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
3
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for LCN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPRF 5792
Affinity Capture-MS Homo sapiens
2 MMP2 4313
Affinity Capture-Western Homo sapiens
3 FCN1 2219
Affinity Capture-MS Homo sapiens
4 P4HB 5034
Two-hybrid Homo sapiens
5 DPPA3  
Affinity Capture-MS Homo sapiens
6 LAIR2  
Two-hybrid Homo sapiens
7 NFAM1  
Affinity Capture-MS Homo sapiens
8 TRIAP1  
Two-hybrid Homo sapiens
9 NRSN1  
Affinity Capture-MS Homo sapiens
10 SCNM1  
Two-hybrid Homo sapiens
11 MBD4  
Affinity Capture-MS Homo sapiens
12 UBE3A 7337
Reconstituted Complex Homo sapiens
13 C4orf26  
Two-hybrid Homo sapiens
14 EBF2  
Affinity Capture-MS Homo sapiens
15 TTC23  
Two-hybrid Homo sapiens
16 LNPEP 4012
Two-hybrid Homo sapiens
17 RPS2P32  
Affinity Capture-MS Homo sapiens
18 ST7  
Affinity Capture-MS Homo sapiens
19 FNDC5  
Affinity Capture-MS Homo sapiens
20 TOR2A 27433
Affinity Capture-MS Homo sapiens
21 PTRH1  
Two-hybrid Homo sapiens
22 USP36  
Affinity Capture-MS Homo sapiens
23 CTDSP2 10106
Two-hybrid Homo sapiens
24 L2HGDH  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 C20orf85  
Two-hybrid Homo sapiens
26 ZDHHC6  
Affinity Capture-MS Homo sapiens
27 PELI1 57162
Two-hybrid Homo sapiens
28 POLL  
Two-hybrid Homo sapiens
29 LY96  
Two-hybrid Homo sapiens
30 EGFL8  
Affinity Capture-MS Homo sapiens
31 NUDT3 11165
Affinity Capture-MS Homo sapiens
32 UGT1A10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 FMNL2 114793
Affinity Capture-MS Homo sapiens
34 UBQLN1 29979
Two-hybrid Homo sapiens
35 SFXN4  
Affinity Capture-MS Homo sapiens
36 THAP10  
Affinity Capture-MS Homo sapiens
37 TCEB3B  
Affinity Capture-MS Homo sapiens
38 PHF11 51131
Affinity Capture-MS Homo sapiens
39 UBE2F  
Two-hybrid Homo sapiens
40 SNX27 81609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 WDR5 11091
Co-localization Homo sapiens
42 CCNC  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
43 C18orf21  
Affinity Capture-MS Homo sapiens
44 CAMLG 819
Two-hybrid Homo sapiens
45 DDX19B 11269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 DDX31  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 VEZF1  
Two-hybrid Homo sapiens
48 C1orf86  
Two-hybrid Homo sapiens
49 BEX2  
Two-hybrid Homo sapiens
50 ST6GALNAC6 30815
Affinity Capture-MS Homo sapiens
51 LRRC10  
Affinity Capture-MS Homo sapiens
52 CERS3  
Affinity Capture-MS Homo sapiens
53 RNF115  
Affinity Capture-MS Homo sapiens
54 ASCL1  
Affinity Capture-MS Homo sapiens
55 MAGED4B  
Two-hybrid Homo sapiens
56 GID8 54994
Affinity Capture-MS Homo sapiens
57 SFMBT1 51460
Affinity Capture-MS Homo sapiens
58 ITLN2  
Affinity Capture-MS Homo sapiens
59 SARAF  
Affinity Capture-MS Homo sapiens
60 UBQLN2 29978
Two-hybrid Homo sapiens
61 TRAPPC2L 51693
Two-hybrid Homo sapiens
62 IQUB  
Affinity Capture-MS Homo sapiens
63 SSUH2  
Affinity Capture-MS Homo sapiens
64 ASB10  
Two-hybrid Homo sapiens
65 RNF25 64320
Affinity Capture-MS Homo sapiens
66 HCN1  
Affinity Capture-MS Homo sapiens
67 APTX  
Affinity Capture-MS Homo sapiens
68 UPK1A 11045
Affinity Capture-MS Homo sapiens
69 GJB5  
Affinity Capture-MS Homo sapiens
70 DKK2  
Affinity Capture-MS Homo sapiens
71 ZBED1  
Two-hybrid Homo sapiens
72 FSTL1 11167
Affinity Capture-MS Homo sapiens
73 ALDH7A1 501
Two-hybrid Homo sapiens
74 RHBDD1 84236
Affinity Capture-MS Homo sapiens
75 TRH  
Two-hybrid Homo sapiens
76 RAMP2  
Two-hybrid Homo sapiens
77 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
78 KMT2A  
Co-localization Homo sapiens
79 AGPAT1 10554
Affinity Capture-MS Homo sapiens
80 ZFPL1 7542
Affinity Capture-MS Homo sapiens
81 NT5C1A  
Affinity Capture-MS Homo sapiens
82 FAM60A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 TMEM176B 28959
Affinity Capture-MS Homo sapiens
85 NPTN 27020
Affinity Capture-MS Homo sapiens
86 PRKAA2 5563
Two-hybrid Homo sapiens
87 PCP4  
Affinity Capture-MS Homo sapiens
88 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
89 NPPA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
91 ZIC1  
Affinity Capture-MS Homo sapiens
92 SGTB  
Two-hybrid Homo sapiens
93 FAM25A  
Two-hybrid Homo sapiens
94 FAM25G  
Two-hybrid Homo sapiens
95 TRIM32 22954
Two-hybrid Homo sapiens
96 UBASH3A 53347
Two-hybrid Homo sapiens
97 HDDC2 51020
Two-hybrid Homo sapiens
98 ALKBH4  
Two-hybrid Homo sapiens
99 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 MSRB3 253827
Two-hybrid Homo sapiens
101 ASPH 444
Two-hybrid Homo sapiens
102 SGTA 6449
Two-hybrid Homo sapiens
103 CLMP 79827
Two-hybrid Homo sapiens
104 MMP9 4318
Affinity Capture-Western Homo sapiens
105 SLC37A4 2542
Affinity Capture-MS Homo sapiens
106 STX17 55014
Affinity Capture-MS Homo sapiens
107 RIPPLY3  
Affinity Capture-MS Homo sapiens
108 UBE2U  
Affinity Capture-MS Homo sapiens
109 OR2A4 79541
Affinity Capture-MS Homo sapiens
110 BMF  
Two-hybrid Homo sapiens
111 SLC25A6 293
Affinity Capture-MS Homo sapiens
112 SEC61G 23480
Two-hybrid Homo sapiens
113 TBC1D21 161514
Two-hybrid Homo sapiens
114 PDIA4 9601
Two-hybrid Homo sapiens
115 SH2D3A  
Affinity Capture-MS Homo sapiens
116 PIN1 5300
Two-hybrid Homo sapiens
117 EHHADH 1962
Two-hybrid Homo sapiens
118 EMC4 51234
Affinity Capture-MS Homo sapiens
119 CDSN 1041
Two-hybrid Homo sapiens
120 TIMM10  
Affinity Capture-MS Homo sapiens
121 C1orf35  
Affinity Capture-MS Homo sapiens
122 TREX2  
Affinity Capture-MS Homo sapiens
123 PTDSS1 9791
Affinity Capture-MS Homo sapiens
124 FAM25C  
Two-hybrid Homo sapiens
125 KLHL11  
Affinity Capture-MS Homo sapiens
126 ERCC3  
Affinity Capture-MS Homo sapiens
127 CHIC2 26511
Two-hybrid Homo sapiens
128 KIR3DS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 NEIL2  
Two-hybrid Homo sapiens
130 KLK10 5655
Affinity Capture-MS Homo sapiens
131 POU4F2  
Two-hybrid Homo sapiens
132 TEFM  
Affinity Capture-MS Homo sapiens
133 TSPAN6 7105
Affinity Capture-MS Homo sapiens
134 PINK1  
Affinity Capture-MS Homo sapiens
135 FOXN3 1112
Affinity Capture-MS Homo sapiens
136 ITGA9 3680
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 OAZ1 4946
Affinity Capture-MS Homo sapiens
138 LCN2 3934
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
139 INSC  
Affinity Capture-MS Homo sapiens
140 GABPA 2551
Affinity Capture-MS Homo sapiens
141 CCR1  
Affinity Capture-MS Homo sapiens
142 CYP3A43  
Affinity Capture-MS Homo sapiens
143 GNG8  
Affinity Capture-MS Homo sapiens
144 CSGALNACT1 55790
Affinity Capture-MS Homo sapiens
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