Gene description for GID8
Gene name GID complex subunit 8
Gene symbol GID8
Other names/aliases C20orf11
TWA1
Species Homo sapiens
 Database cross references - GID8
ExoCarta ExoCarta_54994
Vesiclepedia VP_54994
Entrez Gene 54994
HGNC 15857
MIM 611625
UniProt Q9NWU2  
 GID8 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for GID8
Molecular Function
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IEA
Biological Process
    positive regulation of cell population proliferation GO:0008284 IMP
    Wnt signaling pathway GO:0016055 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IBA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IMP
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cell junction GO:0030054 IDA
 Experiment description of studies that identified GID8 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GID8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFRP2  
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 ARHGAP39  
Affinity Capture-MS Homo sapiens
5 SKI 6497
Affinity Capture-MS Homo sapiens
6 RACGAP1 29127
Affinity Capture-MS Homo sapiens
7 ZMYND19  
Affinity Capture-MS Homo sapiens
8 GID4  
Affinity Capture-MS Homo sapiens
9 CALM1 801
Affinity Capture-MS Homo sapiens
10 HBP1  
Affinity Capture-MS Homo sapiens
11 SLURP1  
Affinity Capture-MS Homo sapiens
12 WDR26 80232
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
13 SNRPF 6636
Affinity Capture-MS Homo sapiens
14 WBP5  
Affinity Capture-MS Homo sapiens
15 POTEF 728378
Affinity Capture-MS Homo sapiens
16 KIAA1429 25962
Affinity Capture-MS Homo sapiens
17 RNPS1 10921
Affinity Capture-MS Homo sapiens
18 RANBP10 57610
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 LECT2  
Affinity Capture-MS Homo sapiens
20 MRPS24 64951
Affinity Capture-MS Homo sapiens
21 GCG  
Affinity Capture-MS Homo sapiens
22 ADCYAP1  
Affinity Capture-MS Homo sapiens
23 FTSJ2 29960
Affinity Capture-MS Homo sapiens
24 MKLN1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PGRMC2 10424
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 ATRX 546
Affinity Capture-MS Homo sapiens
27 SLX4  
Affinity Capture-MS Homo sapiens
28 HTRA2 27429
Affinity Capture-MS Homo sapiens
29 YPEL5 51646
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MRPL4 51073
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 VWA8 23078
Affinity Capture-MS Homo sapiens
32 HSPD1 3329
Co-fractionation Homo sapiens
33 KCTD6  
Affinity Capture-MS Homo sapiens
34 RBMX 27316
Affinity Capture-MS Homo sapiens
35 EPB41L3 23136
Affinity Capture-MS Homo sapiens
36 ARMC8 25852
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
37 GDF15 9518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 CHRDL2 25884
Affinity Capture-MS Homo sapiens
39 Ypel5  
Affinity Capture-MS Mus musculus
40 GID8 54994
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
41 FYN 2534
Affinity Capture-MS Homo sapiens
42 TIGD5  
Affinity Capture-MS Homo sapiens
43 EMILIN3  
Affinity Capture-MS Homo sapiens
44 OPA1 4976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 PNMA2  
Affinity Capture-MS Homo sapiens
46 SPRR2D  
Affinity Capture-MS Homo sapiens
47 TDGF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 WNT10A  
Affinity Capture-MS Homo sapiens
49 ADAMTS1 9510
Affinity Capture-MS Homo sapiens
50 INSL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 DAXX  
Affinity Capture-MS Homo sapiens
52 RMND5A 64795
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
53 GPS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 LY6H  
Affinity Capture-MS Homo sapiens
55 PRG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 MMP7 4316
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 IVL 3713
Affinity Capture-MS Homo sapiens
59 CBFA2T3  
Affinity Capture-MS Homo sapiens
60 PREX1  
Affinity Capture-MS Homo sapiens
61 PRG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 PIGT 51604
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 JPH4 84502
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 LILRB4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CCDC120  
Affinity Capture-MS Homo sapiens
66 CST8  
Affinity Capture-MS Homo sapiens
67 PRKCZ 5590
Affinity Capture-MS Homo sapiens
68 CUL3 8452
Affinity Capture-MS Homo sapiens
69 DUSP26 78986
Affinity Capture-MS Homo sapiens
70 COQ4  
Affinity Capture-MS Homo sapiens
71 SPON1 10418
Affinity Capture-MS Homo sapiens
72 NATD1  
Affinity Capture-MS Homo sapiens
73 RANBP9 10048
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 SOAT1 6646
Affinity Capture-MS Homo sapiens
75 MEX3B 84206
Affinity Capture-MS Homo sapiens
76 Arhgap33  
Affinity Capture-MS Mus musculus
77 GNLY  
Affinity Capture-MS Homo sapiens
78 TBL1X 6907
Co-fractionation Homo sapiens
79 BMP1 649
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 ASH1L 55870
Affinity Capture-MS Homo sapiens
81 MRPL18 29074
Affinity Capture-MS Homo sapiens
82 HUWE1 10075
Affinity Capture-MS Homo sapiens
83 PRICKLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 DDX50 79009
Affinity Capture-MS Homo sapiens
85 Mkln1  
Affinity Capture-MS Mus musculus
86 EFHC2  
Affinity Capture-MS Homo sapiens
87 MRPS2 51116
Affinity Capture-MS Homo sapiens
88 PGRMC1 10857
Affinity Capture-MS Homo sapiens
89 NMB  
Affinity Capture-MS Homo sapiens
90 RMND5B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 AGMAT  
Affinity Capture-MS Homo sapiens
92 Arhgap30  
Affinity Capture-MS Mus musculus
93 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
94 NISCH 11188
Affinity Capture-MS Homo sapiens
95 DDX58 23586
Affinity Capture-RNA Homo sapiens
96 RAB8A 4218
Affinity Capture-MS Homo sapiens
97 MOV10 4343
Affinity Capture-RNA Homo sapiens
98 GM2A 2760
Affinity Capture-MS Homo sapiens
99 TRIM28 10155
Affinity Capture-MS Homo sapiens
100 BRD1 23774
Affinity Capture-MS Homo sapiens
101 SPINK7  
Affinity Capture-MS Homo sapiens
102 LCN2 3934
Affinity Capture-MS Homo sapiens
103 PAFAH1B1 5048
Affinity Capture-MS Homo sapiens
104 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 MAEA 10296
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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